A genetic comparison of pig, cow and trout isolates of Lactococcus garvieae by PFGE analysis
Investigation article published in Letters in Applied Microbiology
December 1st, 2011
Aims:Genetic comparison of Lactococcus garvieae isolated from mammals and fish. Methods and Results: One hundred and ninety seven L. garvieae isolates obtained from trout (n = 153), cow (n = 7) and pigs (n = 37) were genetically characterized by determining their pulsed-field gel electrophoresis (PFGE) profiles after macrorestriction with Bsp120I. Overall, L. garvieae isolates from pigs, cow and trout exhibited distinct PFGE patterns, with a low genetic relationship between them. Isolates from trout generated 2 pulsotypes [Genetic diversity (GD) 0.01], showing that the fish isolates were more genetically homogenous than the others. The L. garvieae isolates from cows displayed 5 (GD 0.71) different pulsotypes while the swine isolates displayed 13 different pulsotypes (GD 0.35). Twenty-one of the 37 swine strains (56.8%) were grouped in a single cluster, which included two closely related (93% similarity) pulsotypes. These pulsotypes exhibited a high frequency of isolation from different organs of the animals, and they were also broadly distributed among herds, suggesting a wide distribution across the swine population. This suggests that L. garvieae might be able to colonize different organs of the swine cardio-respiratory system. Conclusions: Results indicate that most Lactococcus garvieae isolates from pigs and trout exhibited a distinct genetic background
Tejedor JL., Vela AI., Gibello A., Casamayor A., Dominguez L. and Fernandez-Garayzabal JF..
 | Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
| Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
 | Dibaq Diproteg. Grupo Dibaq. |
