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A genetic comparison of pig, cow and trout isolates of Lactococcus garvieae by PFGE analysis

Investigation article published in Letters in Applied Microbiology

December 1st, 2011

Aims:Genetic comparison of Lactococcus garvieae isolated from mammals and fish. Methods and Results:  One hundred and ninety seven L. garvieae isolates obtained from trout (n = 153), cow (n = 7) and pigs (n = 37) were genetically characterized by determining their pulsed-field gel electrophoresis (PFGE) profiles after macrorestriction with Bsp120I. Overall, L. garvieae isolates from pigs, cow and trout exhibited distinct PFGE patterns, with a low genetic relationship between them. Isolates from trout generated 2 pulsotypes [Genetic diversity (GD) 0.01], showing that the fish isolates were more genetically homogenous than the others. The L. garvieae isolates from cows displayed 5 (GD 0.71) different pulsotypes while the swine isolates displayed 13 different pulsotypes (GD 0.35). Twenty-one of the 37 swine strains (56.8%) were grouped in a single cluster, which included two closely related (93% similarity) pulsotypes. These pulsotypes exhibited a high frequency of isolation from different organs of the animals, and they were also broadly distributed among herds, suggesting a wide distribution across the swine population. This suggests that L. garvieae might be able to colonize different organs of the swine cardio-respiratory system. Conclusions:  Results indicate that most Lactococcus garvieae isolates from pigs and trout exhibited a distinct genetic background




Tejedor JL., Vela AI., Gibello A., Casamayor A., Dominguez L. and Fernandez-Garayzabal JF..




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A genetic comparison of pig, cow and trout isolates of Lactococcus garvieae by PFGE analysis

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A genetic comparison of pig, cow and trout isolates of Lactococcus garvieae by PFGE analysis


Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Grupo DibaqDibaq Diproteg. Grupo Dibaq.







Letters in Applied Microbiology
FACTOR YEAR Q
1.622 2011

NLMID: 8510094

PMID: 21933202

ISSN: 0266-8254



TITLE: A genetic comparison of pig, cow and trout isolates of Lactococcus garvieae by PFGE analysis


JOURNAL: Lett. Appl. Microbiol.


NUMERACIÓN: 53(6):614-9


AÑO: 2011


PUBLISHER: Published for the Society for Applied Bacteriology by Blackwell Scientific Publications


AUTHORS: Tejedor JL., Vela AI., Gibello A., Casamayor A., Dominguez L. and Fernandez-Garayzabal JF..


VISAVET PARTICIPANTS


2nd
Ana Isabel Vela Alonso
4th
Almudena Casamayor Sanz
5th
Lucas Domínguez Rodríguez
Last
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/ 10.1111/j.1472-765X.2011.03153.x


CITE THIS PUBLICATION:

Tejedor JL., Vela AI., Gibello A., Casamayor A., Dominguez L. and Fernandez-Garayzabal JF. A genetic comparison of pig, cow and trout isolates of Lactococcus garvieae by PFGE analysis. Letters in Applied Microbiology. 53(6):614-9. 2011. (A). ISSN: 0266-8254. DOI: 10.1111/j.1472-765X.2011.03153.x


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