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A new test to detect antibodies against Mycobacterium tuberculosis complex in red deer serum

The Veterinary Journal publish this investigation article

February 1st, 2019

Red deer (Cervus elaphus) farming is a growing economic activity worldwide. However, the capacity of this species to act as reservoir of animal tuberculosis (TB) poses a threat to other wildlife and to livestock. Diagnostic assay accuracy in this species is therefore highly relevant for prevention and control measures. Our aim was to evaluate the diagnostic performance of the protein complex P22, obtained from Mycobacterium bovis derived purified protein derivative (bPPD), as a candidate antigen for the detection of antibodies against Mycobacterium tuberculosis complex (MTC). We assessed the performance of this new antigen in indirect enzyme-linked immunosorbent assays (ELISA) in TB-positive and TB-negative red deer, in comparison with a bPPD-based ELISA.
The P22 ELISA achieved a higher specificity (Sp) and similar sensitivity (Se) in comparison with the bPPD ELISA at all the cut-off points considered. The P22 ELISA yielded optimal Sp (99.02%; 95% confidence intervals [CI95%]: 96.5–99.8) and appropriate Se (70.1%; CI95%: 63.6–76) at the selected cut-off point of 100%. These results suggest that P22 can be used as an alternative antigen in the immunodiagnosis of animal TB through the use of an ELISA-type detection of antibodies against MTC in red deer, thus contributing to the diagnosis of animal TB in this species as a measure for further disease prevention and control programs




Thomas J., Infantes-Lorenzo JA., Moreno I., Romero B., Garrido F., Juste R., Dominguez M., Dominguez L., Gortazar C. and Risalde MA.




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A new test to detect antibodies against Mycobacterium tuberculosis complex in red deer serum

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A new test to detect antibodies against Mycobacterium tuberculosis complex in red deer serum

Participants:

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Indian Council of Agricultural ResearchIndian Council of Agricultural Research (ICAR).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Gobierno VascoNeiker-Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario. Gobierno Vasco.

Gobierno del Principado de AsturiasServicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA). Gobierno del Principado de Asturias.

Universidad de CórdobaDepartamento de Anatomía y Anatomía Patológica Comparadas. Facultad de Veterinaria. Universidad de Córdoba (UCO).

Instituto Maimonides de Investigación Biomédica de CordobaUnidad de Enfermedades Infecciosas. Hospital Universitario Reina Sofía de Córdoba. Instituto Maimonides de Investigación Biomédica de Cordoba.







The Veterinary Journal
FACTOR YEAR Q
1.773 2017

NLMID: 9706281

PMID: 30825903

ISSN: 1090-0233



TITLE: A new test to detect antibodies against Mycobacterium tuberculosis complex in red deer serum


JOURNAL: Vet. J.


NUMERACIÓN: 15(1):70


AÑO: 2019


PUBLISHER: Elsevier SCI LTD


AUTHORS: Thomas J., Infantes-Lorenzo JA., Moreno I., Romero B., Garrido F., Juste R., Dominguez M., Dominguez L., Gortazar C. and Risalde MA.


VISAVET PARTICIPANTS


2nd
Jose Antonio Infantes Lorenzo
4th
Beatriz Romero Martínez
8th
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1016/j.tvjl.2018.12.021


CITE THIS PUBLICATION:

Thomas J., Infantes-Lorenzo JA., Moreno I., Romero B., Garrido F., Juste R., Dominguez M., Dominguez L., Gortazar C. and Risalde MA. A new test to detect antibodies against Mycobacterium tuberculosis complex in red deer serum. The Veterinary Journal. 15(1):70. 2019. (A). ISSN: 1090-0233. DOI: 10.1016/j.tvjl.2018.12.021


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