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Analysis of the gyrA gene of clinical Yersinia ruckeri isolates with reduced susceptibility to quinolones

Applied and Environmental Microbiology publish this investigation article

January 1st, 2004

Antimicrobial susceptibility of seven clinical strains of Yersinia ruckeri representative of those isolated between 1994 and 2002 from a fish farm with endemic enteric redmouth disease was studied. All isolates displayed indistinguishable pulsed-field gel electrophoresis restriction patterns, indicating that they represented a single strain. However, considering both inhibition zone diameters (IZD) and MICs, the isolates recovered in 2001-2002 formed a separate cluster with lower levels of susceptibility to all the quinolones tested, especially nalidixic acid (NA) and oxolinic acid (OA), compared with the isolates recovered between 1994 and 1998. Analysis of the PCR product of the quinolone resistance-determining region of the gyrA gene from clinical isolates of Y. ruckeri with reduced susceptibility to OA and NA revealed a single amino acid substitution, Ser-83 to Arg-83 (Escherichia coli numbering). Identical substitution was observed in induced OA-resistant mutant strains, which displayed IZD and MICs of quinolones similar to those of the clinical isolates of Y. ruckeri with reduced susceptibility to these antimicrobial agents. These data indicate in that for Y. ruckeri, the substitution of Ser by Arg at position 83 of the gyrA gene is associated with reduced susceptibility to quinolones




Gibello A., Porrero MC., Blanco MM., Vela AI., Liebana P., Moreno MA., Fernandez-Garayzabal JF. and Dominguez L..




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Analysis of the gyrA gene of clinical Yersinia ruckeri isolates with reduced susceptibility to quinolones

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Analysis of the gyrA gene of clinical Yersinia ruckeri isolates with reduced susceptibility to quinolones



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Applied and Environmental Microbiology
FACTOR YEAR Q
3.810 2004

NLMID: 7605801

PMID: 14711693

ISSN: 0099-2240



TITLE: Analysis of the gyrA gene of clinical Yersinia ruckeri isolates with reduced susceptibility to quinolones


JOURNAL: Appl Environ Microbiol


NUMERACIÓN: 70(1):599-602


AÑO: 2004


PUBLISHER: American Society for Microbiology


AUTHORS: Gibello A., Porrero MC., Blanco MM., Vela AI., Liebana P., Moreno MA., Fernandez-Garayzabal JF. and Dominguez L..


4th
Ana Isabel Vela Alonso
7th
José Francisco Fernández-Garayzabal Fernández
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1128/AEM.70.1.599-602.2004


CITE THIS PUBLICATION:

Gibello A., Porrero MC., Blanco MM., Vela AI., Liebana P., Moreno MA., Fernandez-Garayzabal JF. and Dominguez L. Analysis of the gyrA gene of clinical Yersinia ruckeri isolates with reduced susceptibility to quinolones. Applied and Environmental Microbiology. 70(1):599-602. 2004. (A). ISSN: 0099-2240. DOI: 10.1128/AEM.70.1.599-602.2004


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