Poster communication in 4th Med-Vet-Net Annual Scientific Meeting 2015 (Global Challenges in Zoonoses: Combating Emerging Threats using a One-Health Approach)

October 8^th, 2015

Florez-Cuadrado D., Ugarte-Ruiz M., Quesada A., Palomo G., Dominguez L. and Porrero MC.

Thermophilic Campylobacter species, such as C. jejuni and C. coli, are the main cause of bacterial foodborne infection in humans all over the world. Diarrhea of 400-500 million people is estimated to be caused by Campylobacter each year. Even though treatment is not generally required, macrolides (particularly erythromycin) constitute the drug of choice. Thus, development of macrolide resistance poses a serious public health threat. Eighty eight erythromycin-resistant strains according to the EUCAST cut-off values (MIC>8 mg/L; broth microdilution) from different sources (poultry, swine, cattle and sewage) were selected. The aim of this study was to analyse a wider erythromycin-resistance range (from 16 to 1024 mg/L) and compare the antimicrobial resistance data (to erythromycin and others) among the Campylobacter strains from different sources. Our results show erythromycin-resistance levels between 32 and 1024 mg/L. The majority of Campylobacter strains with Ery-MIC=32 mg/L are from sewage (52%; 11/21). Strains with Ery-MIC between 64 and 512 mg/L are mostly from swine (56%: 34/61) and Campylobacter strains with highest levels of erythromycin-resistance (MIC≥1024 mg/L) are all from poultry (100%; 6/6). According to our results, Campylobacter strains with highest erythromycin-resistance levels are those from poultry and the lowest resistance levels are shown by Campylobacter strains from sewage. Additionally, the majority of the Campylobacter strains with higher erythromycin-resistance level (MIC≥512 mg/L) are also resistant to gentamicin (79%; 11/14)

	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Veterinaria. Universidad de Extremadura (UNEX).