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Cytokines gene expression detection by real time RT-PCR in sheep

Conference in VIII Congreso de Ciencias Veterinarias y Biomédicas: Facultad de Veterinaria

April 27th, 2009

Perez de Diego AC., Sanchez-Matamoros A. and Sanchez-Vizcaino JM.

Cytokines are protein molecules that cells from the immune system secrete in response to immune stimulation. There are different techniques to study their production and their action on individuals. This study consists of the development of a real time RT-PCR method for the detection of the expression of mRNA coding for six cytokines (TNF-α, IFNγ, IL-4, IL2, IL-10, IL-12). The preliminary results were obtained from the study of lymphocites stimulated with Con A and LPS, showing the detection of ARNm, IL-4, IL-2, IFNγ and TNF-α, IL-12, IL-10, respectively. Its subsequent application in sheep that were vaccinated against Chlamydophila




Participants:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).


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Cytokines gene expression detection by real time RT-PCR in sheep


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Publication: Cytokines gene expression detection by real time RT-PCR in sheep

Link to VIII Congreso de Ciencias Veterinarias y Biomédicas: Facultad de Veterinaria





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VIII Congreso de Ciencias Veterinarias y Biomédicas: Facultad de Veterinaria


VIII Congreso de Ciencias Veterinarias y Biomédicas: Facultad de Veterinaria
April 27th-29th, 2009
Madrid
Spain

TITLE: Detecciónes de la expresión de citoquinas mediante RT-PCR en tiempo real en ganado ovino


TYPE: Oral communication


AUTHORS: Perez de Diego AC., Sanchez-Matamoros A. and Sanchez-Vizcaino JM.


VISAVET PARTICIPANTS


José Manuel Sánchez-Vizcaíno Rodríguez

DATE: April 27th, 2009


CITE THIS COMMUNICATION:

Perez de Diego AC., Sanchez-Matamoros A. and Sanchez-Vizcaino JM. Cytokines gene expression detection by real time RT-PCR in sheep. VIII Congreso de Ciencias Veterinarias y Biomédicas: Facultad de Veterinaria, Facultad de Veterinaria. Universidad Complutense, Madrid, Spain, April 27th, 2009. (Oral communication)


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