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Detection of Leishmania infantum in samples of wild Leporidae by Direct Fluorescent Antibody (DFA) assay

Poster presented in Med-Vet-Net Association 5th International Scientific Conference (OneHealth: Zoonoses - Emerging Threats)

June 27th, 2017

Ortega-García MV., Moreno I., Garcia N., Dominguez M. and Salguero FJ.

Leishmania infantum is a protozoan parasite and one of the causative agent of leishmaniosis, a zoonosis and a neglected tropical disease (NTD). Canines are the main domestic animal reservoir, but recently, Leporidae have been described as competent reservoirs, being the source of infection during an important outbreak in Madrid (Spain) that has involved 697 human cases from 2009 to 2016, and it is still active. The detection of leishmaniosis in wild animals becomes a great challenge during surveillance programs in One Health initiatives. Therefore, the study of microscopic lesions and the detection of L. infantum by immunofluorescence staining in different tissues could add further insight onto the disease in wild Leporidae and would permit the precise localization of L. infantum in tissues for an eventual and more accurate DNA extraction process.




Participants:

Ministerio de DefensaCampus La Marañosa (ITM). Instituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa.

Instituto de Salud Carlos IIICentro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

University of SurreySchool of Veterinary Medicine. Faculty of Health and Medical Sciences. University of Surrey.


Link to Med-Vet-Net Association 5th International Scientific Conference (OneHealth: Zoonoses - Emerging Threats)





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Med-Vet-Net Association 5th International Scientific Conference (OneHealth: Zoonoses - Emerging Threats)


Med-Vet-Net Association 5th International Scientific Conference (OneHealth: Zoonoses - Emerging Threats)
Jun 27th-29th, 2017
Guildford
United Kingdom

TITLE: Detection of Leishmania infantum in samples of wild Leporidae by Direct Fluorescent Antibody (DFA) assay


TYPE: Poster communication


AUTHORS: Ortega-García MV., Moreno I., Garcia N., Dominguez M. and Salguero FJ.


VISAVET PARTICIPANTS


First
Mª Victoria Ortega García
3rd
Nerea García Benzaquén

DATE: June 27th, 2017


CITE THIS COMMUNICATION:

Ortega-García MV., Moreno I., Garcia N., Dominguez M. and Salguero FJ. Detection of Leishmania infantum in samples of wild Leporidae by Direct Fluorescent Antibody (DFA) assay. Med-Vet-Net Association 5th International Scientific Conference (OneHealth: Zoonoses - Emerging Threats), Med-Vet-Net Association, Guildford, United Kingdom, June 27th, 2017. (Poster communication)


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