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Diagnostic performance of PCR and ELISA on blood and milk samples and serological survey for small ruminant lentiviruses in central Spain

Investigation published in The Veterinary Record

January 4th, 2011

The diagnostic performance of an ELISA for the detection of antibodies to the small ruminant lentiviruses (SRLVs) maedi-visna virus and caprine arthritis-encephalitis virus in milk and corresponding blood samples was evaluated in 50 sheep. The agreement between ELISA results in blood and milk was 90 per cent, and the κ value was 0.79. In addition, a serological survey in the central zone of Spain was performed using milk samples from 413 animals (250 sheep and 163 goats) from 12 flocks/herds. All flocks/herds had some animals that were positive for SRLV. Among the animals, 60.0 per cent of the sheep and 8.0 per cent of the goats tested were seropositive. Each sample was also tested using a PCR technique, which increased the percentage of positive animals detected. Using a combination of ELISA and PCR gave a total of 72.2 per cent of sheep and 28.8 per cent of goats positive for SRLV




Barquero N., Arjona A., Domenech A., Toural C., Las Heras A., Fernandez-Garayzabal JF., Ruiz-Santa Quiteria JA. and Gomez-Lucia E.




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Diagnostic performance of PCR and ELISA on blood and milk samples and serological survey for small ruminant lentiviruses in central Spain

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Diagnostic performance of PCR and ELISA on blood and milk samples and serological survey for small ruminant lentiviruses in central Spain



Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







The Veterinary Record
FACTOR YEAR Q
1.248 2011

NLMID: 31164

PMID: 21257533

ISSN: 0042-4900



TITLE: Diagnostic performance of PCR and ELISA on blood and milk samples and serological survey for small ruminant lentiviruses in central Spain


JOURNAL: Vet Rec


NUMERACIÓN: 168(1):20


AÑO: 2011


PUBLISHER: British Veterinary Association


AUTHORS: Barquero N., Arjona A., Domenech A., Toural C., Las Heras A., Fernandez-Garayzabal JF., Ruiz-Santa Quiteria JA. and Gomez-Lucia E.


José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1136/vr.c4951


CITE THIS PUBLICATION:

Barquero N., Arjona A., Domenech A., Toural C., Las Heras A., Fernandez-Garayzabal JF., Ruiz-Santa Quiteria JA. and Gomez-Lucia E. Diagnostic performance of PCR and ELISA on blood and milk samples and serological survey for small ruminant lentiviruses in central Spain. The Veterinary Record. 168(1):20. 2011. (A). ISSN: 0042-4900. DOI: 10.1136/vr.c4951


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