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Diarrheagenic Escherichia coli Pathotypes From Children Younger Than 5 Years in Kano State, Nigeria

Frontiers in Public Health publish this investigation article

November 27th, 2019

Diarrheagenic Escherichia coli (DEC) is one of the leading causes of gastrointestinal disorders worldwide and an important public health challenge. DEC infection is often underdiagnosed during routine microbiological analysis, especially in resource constrained settings; the use of molecular tests could however help to determine the distribution of DEC and its clinical significance. Here, a study to assess the prevalence of DEC in clinical samples from patients <5 years attending three hospitals in Kano state, Nigeria, was carried out. Samples from 400 patients and 50 controls were collected and screened for E. coli. Compatible colonies from 248 individuals (215 patients and 33 controls) were characterized using biochemical test, a set of real-time PCRs for detection of nine virulence factors (VF: eae, bfpA, elt, est, stx1, stx2, ehxA, aggR, and invA) associated with five DEC pathotypes (EPEC, ETEC, EHEC, EAEC, and EIEC) and antimicrobial susceptibility tests. One or more VFs typical of specific pathotypes were detected in 73.8% (183/248) of the isolates, with those associated with EAEC (36.3%), ETEC (17.3%), and EPEC (6.0%) being the most common, although proportion of specific pathotypes differed between hospitals. est was the only VF detected in a significantly higher proportion in cases compared to controls (P = 0.034). Up to 86.9% of DEC were resistant to at least one class of antibiotics, with trimethoprim-sulfamethoxazole being the least effective drug (77.6% resistance). Our results demonstrate the widespread circulation of different DEC pathotypes that were highly resistant to trimethoprim-sulfamethoxazole among children in Kano state, and highlight the need of characterizing the causative agents in cases of gastrointestinal disorders




Saka, K.H., Dabo NT., Muhammad B., Garcia-Soto S., Ugarte-Ruiz M. and Alvarez J..




See this article
Diarrheagenic Escherichia coli Pathotypes From Children Younger Than 5 Years in Kano State, Nigeria




Participants:

Nigerian Stored Products Research InstituteNigerian Stored Products Research Institute (NSPRI).

Bayero University KanoDepartment of Microbiology. Bayero University Kano (BUK).

Bayero University KanoDepartment of Biological Sciences. Bayero University Kano (BUK).

Universidad ComplutenseCentro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Frontiers in Public Health
FACTOR YEAR Q
2.031 2018

NLMID: 101616579

ISSN: 2296-2565



TITLE: Diarrheagenic Escherichia coli Pathotypes From Children Younger Than 5 Years in Kano State, Nigeria


JOURNAL: Front Public Health


NUMERACIÓN: 7:348


AÑO: 2019


PUBLISHER: Lausanne : Frontiers Editorial Office


AUTHORS: Saka, K.H., Dabo NT., Muhammad B., Garcia-Soto S., Ugarte-Ruiz M. and Alvarez J..


VISAVET PARTICIPANTS


5th
María Ugarte Ruiz
Last
Julio Álvarez Sánchez

DOI: https://doi.org/ 10.3389/fpubh.2019.00348


CITE THIS PUBLICATION:

Saka, K.H., Dabo NT., Muhammad B., Garcia-Soto S., Ugarte-Ruiz M. and Alvarez J. Diarrheagenic Escherichia coli Pathotypes From Children Younger Than 5 Years in Kano State, Nigeria. Frontiers in Public Health. 7:348. 2019. (A). ISSN: 2296-2565. DOI: 10.3389/fpubh.2019.00348