Home \ Research \ Scientific publications \


Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis

Investigation published in Veterinary Research

July 27th, 2018

Animal tuberculosis (TB) remains a major problem in some countries despite the existence of control programmes focused mainly on cattle. In this species, aerogenous transmission is accepted as the most frequent infection route, affecting mainly the respiratory system. Under the hypothesis that the oral route could be playing a more relevant role in transmission, diagnosis and disease persistence than previously thought, this study was performed to assess the course of TB infection in cattle and its effects on diagnosis depending on the route of entry of Mycobacterium bovis. Two groups of five calves each were either endotracheally (EC) or orally (OC) challenged. Necropsies were carried out 12 weeks after challenge except for three OC calves slaughtered 8 weeks later. All animals reacted to the tuberculin skin test and the entire EC group was positive to the interferon-gamma release assay (IGRA) 2 weeks after challenge and thereafter. The first positive IGRA results for OC calves (3/5) were recorded 4 weeks after challenge. Group comparison revealed significant differences in lesion and positive culture location and scoring. TB-compatible gross lesions and positive cultures were more frequently found in the thorax (p < 0.001) and lung (p < 0.05) of EC animals, whereas OC animals presented lesions (p = 0.23) and positive cultures (p < 0.05) mainly located in the abdomen. These results indicate that the infection route seems to be a determining factor for both the distribution and the time needed for the development of visible lesions. Our study suggests that confirmation of TB infection in some skin reactor animals can be problematic if current post-mortem examination and diagnostics are not improved




Serrano M., Sevilla AI., Fuertes M., Geijo M., Risalde MA., Ruiz-Fons F., Gortazar C., Juste RA., Dominguez L., Elguezabal N. and Garrido JM.




See this article
Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis

See it on NLM PubMed
Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis



Participants:

Gobierno VascoAnimal Health Department. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Gobierno Vasco.

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidad de CórdobaDepartamento de Anatomía y Anatomía Patológica Comparadas. Facultad de Veterinaria. Universidad de Córdoba (UCO).

Gobierno del Principado de AsturiasServicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA). Gobierno del Principado de Asturias.

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







FACTOR YEAR Q
3.117 2018

NLMID: 9309551

PMID: 30053884

ISSN: 0928-4249



TITLE: Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis


JOURNAL: Vet Res


NUMERACIÓN: 49(1):74


AÑO: 2018


PUBLISHER: BMC


AUTHORS: Serrano M., Sevilla AI., Fuertes M., Geijo M., Risalde MA., Ruiz-Fons F., Gortazar C., Juste RA., Dominguez L., Elguezabal N. and Garrido JM.


VISAVET PARTICIPANTS


Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1186/s13567-018-0566-2


CITE THIS PUBLICATION:

Serrano M., Sevilla AI., Fuertes M., Geijo M., Risalde MA., Ruiz-Fons F., Gortazar C., Juste RA., Dominguez L., Elguezabal N. and Garrido JM. Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis. Veterinary Research. 49(1):74. 2018. (A). ISSN: 0928-4249. DOI: 10.1186/s13567-018-0566-2


UNITS: