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Evaluation of the P22 ELISA for the detection of Mycobacterium bovis-specific antibodies in oral fluid samples obtained from goats

Investigation article published in Frontiers in veterinary science

August 11st, 2021

The ante-mortem diagnosis of tuberculosis (TB) in ruminants is based mainly on the intradermal tuberculin test and the IFN-γ assay. Antibody (Ab)-based tests have emerged as potential tools for the detection of TB infected animals using serum, plasma or even milk samples. Oral fluids have also been evaluated as alternative samples with which to detect specific Abs against M. bovis in pigs or wild boars, but not in ruminants. The objective of this study was, therefore, to evaluate the performance of an in house-ELISA for TB diagnosis (P22 ELISA) in goats as an experimental model for the diagnosis of TB using oral fluid samples.
Oral fluid samples from 64 goats from a TB-infected herd (n=197) and all the animals from a TB-free herd (n=113) were analysed using the P22 ELISA. The estimated sensitivity (Se) and specificity (Sp) were 34.4% (95% CI 22.4-45.6) and 100% (95% CI 97.4-100), respectively. The optimal cut-off point was set at 100% according to the ROC analysis. Those animals with a higher level of Abs in their oral fluid attained a higher lesion score (p=0.018). In fact, when taking into account only the setting of the animals with severe lesions (n=16), the ELISA showed a Se of 75% (95% CI 53.7-96.2).
Results of the present study suggest that the P22 ELISA is highly specific but has a limited value detecting infected animals in oral fluid samples. Nevertheless, its performance is significantly higher in the presence of severe lesions




Ortega-Martin J., Infantes-Lorenzo JA., Bezos J., Roy A., de Juan L., Romero B., Moreno I., Gómez-Buendía A., Agullo-Ros I., Dominguez L. and Dominguez M.




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Evaluation of the P22 ELISA for the detection of Mycobacterium bovis-specific antibodies in oral fluid samples obtained from goats

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Evaluation of the P22 ELISA for the detection of Mycobacterium bovis-specific antibodies in oral fluid samples obtained from goats



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Instituto de Salud Carlos IIICentro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad de CórdobaUniversidad de Córdoba (UCO).







Frontiers in veterinary science
FACTOR YEAR Q
2.245 2019

NLMID: 101666658

PMID: 34458351

ISSN: 2297-1769



TITLE: Evaluation of the P22 ELISA for the detection of Mycobacterium bovis-specific antibodies in oral fluid samples obtained from goats


JOURNAL: Front Vet Sci


NUMERACIÓN: 8:674636


AÑO: 2021


PUBLISHER: Lausanne : Frontiers Media S.A


AUTHORS: Ortega-Martin J., Infantes-Lorenzo JA., Bezos J., Roy A., de Juan L., Romero B., Moreno I., Gómez-Buendía A., Agullo-Ros I., Dominguez L. and Dominguez M.


VISAVET PARTICIPANTS


First
Javier Ortega Martín
3rd
Javier Bezos Garrido
5th
Lucía de Juan Ferré
6th
Beatriz Romero Martínez
8th
Alberto Gómez Buendía
10
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.3389/fvets.2021.674636


CITE THIS PUBLICATION:

Ortega-Martin J., Infantes-Lorenzo JA., Bezos J., Roy A., de Juan L., Romero B., Moreno I., Gómez-Buendía A., Agullo-Ros I., Dominguez L. and Dominguez M. Evaluation of the P22 ELISA for the detection of Mycobacterium bovis-specific antibodies in oral fluid samples obtained from goats. Frontiers in veterinary science. 8:674636. 2021. (A). ISSN: 2297-1769. DOI: 10.3389/fvets.2021.674636


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