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Evaluation of two cocktails containing ESAT-6, CFP-10 and Rv-3615c in the intradermal test and the interferon-gamma assay for diagnosis of bovine tuberculosis

Preventive Veterinary Medicine publish this investigation article

June 1st, 2012

The intradermal tuberculin tests and the interferon-gamma (IFN-γ) assay are the principal tests used worldwide for the ante-mortem diagnosis of bovine tuberculosis. The conventional reagent currently in use in these tests is purified protein derivative (PPD) tuberculin obtained from Mycobacterium bovis culture. The components of PPD are poorly characterized and difficult to standardize. To overcome this issue, antigens specific to the Mycobacterium tuberculosis complex are being studied. Here we have assessed the biological potency of ESAT-6, CFP-10 and Rv-3615c presented as peptide or recombinant protein cocktails in comparison with the standard bovine PPD used routinely in Spanish eradication campaigns. The study was performed in cattle (n=23) from a herd with natural M. bovis infection. Animals were simultaneously injected with PPD and the peptide and protein cocktails. The percentages of cattle reacting positively to single intradermal test were 60.9% (bovine PPD), 47.8% (peptide cocktail) and 60.9% (protein cocktail), with no significant difference between the actual skin fold thickness increases (p>0.05). The IFN-γ assay detected 60.9% of animals when stimulation was performed with bovine PPD, but decreased to 52.2% when stimulation was performed with the peptide cocktail and to 47.8% when stimulation was performed with the protein cocktail. However, no significant differences were found between IFN-γ responder frequencies (p>0.05). These results show a potential use of these defined reagents for in vivo tuberculosis diagnosis




Casal C., Bezos J., Diez-Guerrier A., Alvarez J., Romero B., de Juan L., Rodriguez-Campos S., Vordermeier M., Whelan A., Hewinson RG., Mateos A., Dominguez L. and Aranaz A.




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Evaluation of two cocktails containing ESAT-6, CFP-10 and Rv-3615c in the intradermal test and the interferon-gamma assay for diagnosis of bovine tuberculosis

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Evaluation of two cocktails containing ESAT-6, CFP-10 and Rv-3615c in the intradermal test and the interferon-gamma assay for diagnosis of bovine tuberculosis


Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaInstituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Department for Environment, Food and Rural AffairsDepartment for Bovine Tuberculosis. Animal Health and Veterinary Laboratories Agency (AHVLA). Department for Environment, Food and Rural Affairs (Defra).







Preventive Veterinary Medicine
FACTOR YEAR Q
2.389 2012

NLMID: 8217463

PMID: 22391021

ISSN: 0167-5877



TITLE: Evaluation of two cocktails containing ESAT-6, CFP-10 and Rv-3615c in the intradermal test and the interferon-gamma assay for diagnosis of bovine tuberculosis


JOURNAL: Prev. Vet. Med.


NUMERACIÓN: 105(1-2):149-154


AÑO: 2012


PUBLISHER: Elsevier Science Publishers


AUTHORS: Casal C., Bezos J., Diez-Guerrier A., Alvarez J., Romero B., de Juan L., Rodriguez-Campos S., Vordermeier M., Whelan A., Hewinson RG., Mateos A., Dominguez L. and Aranaz A.


VISAVET PARTICIPANTS


2nd
Javier Bezos Garrido
3rd
Alberto Antoine Díez Guerrier
4th
Julio Álvarez Sánchez
5th
Beatriz Romero Martínez
6th
Lucía de Juan Ferré
12th
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1016/j.prevetmed.2012.02.007


CITE THIS PUBLICATION:

Casal C., Bezos J., Diez-Guerrier A., Alvarez J., Romero B., de Juan L., Rodriguez-Campos S., Vordermeier M., Whelan A., Hewinson RG., Mateos A., Dominguez L. and Aranaz A. Evaluation of two cocktails containing ESAT-6, CFP-10 and Rv-3615c in the intradermal test and the interferon-gamma assay for diagnosis of bovine tuberculosis. Preventive Veterinary Medicine. 105(1-2):149-154. 2012. (A). ISSN: 0167-5877. DOI: 10.1016/j.prevetmed.2012.02.007


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