Veterinary Microbiology publish this investigation article

June 25^th, 2014

Zebrafish has been used for studying infections and host-pathogen interactions in different bacterial fish pathogens. In the present study we evaluated the ability of Lactococcus garvieae to infect zebrafish when inoculated intraperitoneally with 2×107UFC of this pathogen. L. garvieae can colonize and invade zebrafish at multiple anatomical sites causing a lethal acute septicemic infection with clinical signs and lesions consistent with those observed in lactococcosis outbreaks. Immunohistochemical studies showed the presence of L. garvieae into macrophages as well as into non-phagocytic zebrafish cells of liver (hepatocytes). The internalization capacity showed by L. garvieae in zebrafish cells was confirmed in the rainbow trout cell line RTG-2. Our results provide the first evidence that L. garvieae is able to invade non-phagocytic host cells

Aguado-Urda M., Rodriguez-Bertos A., Heras AI., Blanco MM., Acosta F., Cid R., Fernandez-Garayzabal JF. and Gibello A.

Experimental Lactococcus garvieae infection in zebrafish and first evidence of its ability to invade non-phagocytic cells

Experimental Lactococcus garvieae infection in zebrafish and first evidence of its ability to invade non-phagocytic cells

	Servicio de Identificación y Caracterización Microbiana (ICM). Servicio de Patología y Veterinaria Forense (SAP). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Departamento de Medicina y Cirugía Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Bioorganic Research and Services S.A. (BIONATURIS).
	Universidad de Las Palmas de Gran Canaria (ULPGC).