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Garvicins AG1 and AG2: Two Novel Class IId Bacteriocins of Lactococcus garvieae Lg-Granada

International Journal of Molecualr Sciences publish this investigation article

April 23rd, 2022

Lactococcus garvieae causes infectious diseases in animals and is considered an emerging zoonotic pathogen involved in human clinical conditions. In silico analysis of plasmid pLG50 of L. garvieae Lg-Granada, an isolate from a patient with endocarditis, revealed the presence of two gene clusters (orf46-47 and orf48-49), each one encoding a novel putative bacteriocin, i.e., garvicin AG1 (GarAG1; orf46) and garvicin AG2 (GarAG2; orf48), and their corresponding immunity proteins (orf47 and orf49). The chemically synthesised bacteriocins GarAG1 and GarAG2 presented inhibitory activity against pathogenic L. garvieae strains, with AG2 also being active against Listeria monocytogenes, Listeria ivanovii and Enterococcus faecalis. Genetic organisation, amino acid sequences and antimicrobial activities of GarAG1 and GarAG2 indicate that they belong to linear non-pediocin-like one-peptide class IId bacteriocins. Gram-positive bacteria that were sensitive to GarAG2 were also able to ferment mannose, suggesting that this bacteriocin could use the mannose phosphotransferase transport system (Man-PTS) involved in mannose uptake as a receptor in sensitive strains. Intriguingly, GarAG1 and GarAG2 were highly active against their own host, L. garvieae Lg-Granada, which could be envisaged as a new strategy to combat pathogens via their own weapons




Maldonado-Barragan A., Alegria-Carrasco E., Blanco MM., Vela AI., Fernandez-Garayzabal JF., Rodriguez JM. and Gibello A.




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Garvicins AG1 and AG2: Two Novel Class IId Bacteriocins of Lactococcus garvieae Lg-Granada

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Garvicins AG1 and AG2: Two Novel Class IId Bacteriocins of Lactococcus garvieae Lg-Granada



Participants:

University of St AndrewsInfection and Global Health Division. School of Medicine. University of St Andrews.

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Nutrición, Bromatología y Tecnología de los Alimentos. Facultad de Veterinaria. Universidad Complutense (UCM).







International Journal of Molecualr Sciences
FACTOR YEAR Q
5.924 2020

PMID: 35563074

ISSN: 1422-0067



TITLE: Garvicins AG1 and AG2: Two Novel Class IId Bacteriocins of Lactococcus garvieae Lg-Granada


JOURNAL: Int. J. Mol. Sci


NUMERACIÓN: 23(9):4685


AÑO: 2022


PUBLISHER: MDPI


AUTHORS: Maldonado-Barragan A., Alegria-Carrasco E., Blanco MM., Vela AI., Fernandez-Garayzabal JF., Rodriguez JM. and Gibello A.


VISAVET PARTICIPANTS


4th
Ana Isabel Vela Alonso
5th
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.3390/ijms23094685


CITE THIS PUBLICATION:

Maldonado-Barragan A., Alegria-Carrasco E., Blanco MM., Vela AI., Fernandez-Garayzabal JF., Rodriguez JM. and Gibello A. Garvicins AG1 and AG2: Two Novel Class IId Bacteriocins of Lactococcus garvieae Lg-Granada. International Journal of Molecualr Sciences. 23(9):4685. 2022. (A). ISSN: 1422-0067. DOI: 10.3390/ijms23094685


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