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Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States

Investigation published in mSphere

October 28th, 2020

Fluoroquinolones and cephalosporins are critically important antimicrobial classes for both human and veterinary medicine. We previously found a drastic increase in enrofloxacin resistance in clinical Escherichia coli isolates collected from diseased pigs from the United States over 10 years (2006 to 2016). However, the genetic determinants responsible for this increase have yet to be determined. The aim of the present study was to identify and characterize the genetic basis of resistance against fluoroquinolones (enrofloxacin) and extended-spectrum cephalosporins (ceftiofur) in swine E. coli isolates using whole-genome sequencing (WGS). blaCMY-2 (carried by IncA/C2, IncI1, and IncI2 plasmids), blaCTX-M (carried by IncF, IncHI2, and IncN plasmids), and blaSHV-12 (carried by IncHI2 plasmids) genes were present in 87 (82.1%), 19 (17.9%), and 3 (2.83%) of the 106 ceftiofur-resistant isolates, respectively. Of the 110 enrofloxacin-resistant isolates, 90 (81.8%) had chromosomal mutations in gyrA, gyrB, parA, and parC genes. Plasmid-mediated quinolone resistance genes [qnrB77, qnrB2, qnrS1, qnrS2, and aac-(6)-lb′-cr] borne on ColE, IncQ2, IncN, IncF, and IncHI2 plasmids were present in 24 (21.8%) of the enrofloxacin-resistant isolates. Virulent IncF plasmids present in swine E. coli isolates were highly similar to epidemic plasmids identified globally. High-risk E. coli clones, such as ST744, ST457, ST131, ST69, ST10, ST73, ST410, ST12, ST127, ST167, ST58, ST88, ST617, ST23, etc., were also found in the U.S. swine population. Additionally, the colistin resistance gene (mcr-9) was present in several isolates. This study adds valuable information regarding resistance to critical antimicrobials with implications for both animal and human health




Singh S.H., Lim S., Hong S., Elnekave E., Johnson T., Rovira A., Vannucci F., Clayton JB., Perez A. and Alvarez J..




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Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States

See it on NLM PubMed
Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States


Participants:

University of MinnesotaDepartment of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota (UMM).

Department of Biology. University of Nebraska at Omaha.

University of MinnesotaBioinformatics and Computational Biology Program. University of Minnesota (UMM).

Department of Microbiology and Immunology. Rega Institute. KU Leuven—University of Leuven.

University of MinnesotaDepartment of Veterinary and Biomedical Sciences. Veterinary Diagnostic Laboratory. College of Veterinary Medicine. University of Minnesota (UMM).

Nebraska Food for Health Center. University of Nebraska.

Universidad ComplutenseCentro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







mSphere
FACTOR YEAR Q
4.282 2019

NLMID: 101674533

PMID: 33115839

ISSN: 2379-5042



TITLE: Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States


JOURNAL: mSphere


NUMERACIÓN: 5(5):e00990-20


AÑO: 2020


PUBLISHER: American Society for Microbiology


AUTHORS: Singh S.H., Lim S., Hong S., Elnekave E., Johnson T., Rovira A., Vannucci F., Clayton JB., Perez A. and Alvarez J..


VISAVET PARTICIPANTS


Julio Álvarez Sánchez

DOI: https://doi.org/ 10.1128/mSphere.00990-20


CITE THIS PUBLICATION:

Singh S.H., Lim S., Hong S., Elnekave E., Johnson T., Rovira A., Vannucci F., Clayton JB., Perez A. and Alvarez J. Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States. mSphere. 5(5):e00990-20. 2020. (A). ISSN: 2379-5042. DOI: 10.1128/mSphere.00990-20