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Mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing of SB0121, the most frequent spoligotype in Spain

Oral communication in Workshop "VNTR/MIRU and DVR-Spoligotyping for Mycobacterium bovis typing"

March 25th, 2009

Rodriguez-Campos S., Castellanos E., de Juan L., Bezos J., Gallardo F., Moya N., Alvarez J., Alvarez N., Alende T., Gutierrez A., Lozano F., Mateos A. and Romero B.

Among Spanish Mycobacterium (M.) bovis isolates the most prevalent spoligotype pattern is SB0121, which contains all M. bovis spacers except number 21. This spoligotype accounts for 27.9% of the M. bovis isolates and has been found throughout the country over the last 16 years. Furthermore, this spoligotype, which represents almost 30% of the cattle isolates, is also detected in goats and wildlife (red deer, fallow deer and wild boar). Other Mediterranean countries such as France, Portugal and Italy also described the isolation of SB0121, as well as reports from South Africa and South America. In contrast, no SB0121 isolates were reported from the British Isles. In order to determine whether the high prevalence of this spoligotype can be due to incapacity of the DVR-spoligotyping to further discriminate these strains, we analysed a representative panel of SB0121 isolates (n=100) by MIRU-VNTR typing. A group of six loci was used to subtype the panel: ETRA (VNTR2165), ETRB (VNTR2461), ETRD (VNTR580), QUB11b
(VNTR2163b), QUB3232 (VNTR3232, short fragment) and QUB26 (VNTR4052). Five of the loci were included in the Multilocus Variable Number Tandem Repeat Genotyping of M. bovis Ring Trial VENoMYC 2006, and QUB26 was chosen because by our experience it provides a better discrimination than QUB11a (VNTR2163a). A differentiation into 55 profiles was achieved, with a discriminatory power (D) of 0.97. The three most discriminative loci in decreasing order were: VNTR3232, ETRA and ETRB. This set of loci resulted highly discriminative for the Spanish M. bovis SB0121 spoligotype and therefore indicates the lack of discriminatory power of DVR- spoligotyping in this particular case




Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).


Link to Workshop "VNTR/MIRU and DVR-Spoligotyping for Mycobacterium bovis typing"





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Workshop "VNTR/MIRU and DVR-Spoligotyping for Mycobacterium bovis typing"


Workshop
March 24th-25th, 2009
Madrid
Spain

TITLE: Mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing of SB0121, the most frequent spoligotype in Spain


TYPE: Oral communication


AUTHORS: Rodriguez-Campos S., Castellanos E., de Juan L., Bezos J., Gallardo F., Moya N., Alvarez J., Alvarez N., Alende T., Gutierrez A., Lozano F., Mateos A. and Romero B.


VISAVET PARTICIPANTS


3rd
Lucía de Juan Ferré
4th
Javier Bezos Garrido
6th
Nuria Moya Álvarez
7th
Julio Álvarez Sánchez
9th
Tatiana Alende García
10
Alexandra Gutiérrez Tobaruela
11th
Francisco Javier Lozano Barrilero
Last
Beatriz Romero Martínez

DATE: March 25th, 2009


CITE THIS COMMUNICATION:

Rodriguez-Campos S., Castellanos E., de Juan L., Bezos J., Gallardo F., Moya N., Alvarez J., Alvarez N., Alende T., Gutierrez A., Lozano F., Mateos A. and Romero B. Mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing of SB0121, the most frequent spoligotype in Spain. Workshop "VNTR/MIRU and DVR-Spoligotyping for Mycobacterium bovis typing", Centro de Vigilancia Sanitaria Veterinaria. Universidad Complutense, Madrid, Spain, March 25th, 2009. (Oral communication)


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