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Novel adenovirus detected in captive bottlenose dolphins (Tursiops truncatus) suffering from self-limiting gastroenteritis

BMC Veterinary Research publish this investigation article

March 7th, 2015

Adenoviruses are common pathogens in vertebrates, including humans. In marine mammals, adenovirus has been associated with fatal hepatitis in sea lions. However, only in rare cases have adenoviruses been detected in cetaceans, where no clear correlation was found between presence of the virus and disease status.
A novel adenovirus was identified in four captive bottlenose dolphins with self-limiting gastroenteritis. Viral detection and identification were achieved by: PCR-amplification from fecal samples; sequencing of partial adenovirus polymerase (pol) and hexon genes; producing the virus in HeLa cells, with PCR and immunofluorescence detection, and with sequencing of the amplified pol and hexon gene fragments. A causative role of this adenovirus for gastroenteritis was suggested by: 1) we failed to identify other potential etiological agents; 2) the exclusive detection of this novel adenovirus and of seropositivity for canine adenoviruses 1 and 2 in the four sick dolphins, but not in 10 healthy individuals of the same captive population; and 3) the virus disappeared from feces after clinical signs receded. The partial sequences of the amplified fragments of the pol and hexon genes were closest to those of adenoviruses identified in sea lions with fatal adenoviral hepatitis, and to a Genbank-deposited sequence obtained from a harbour porpoise.
These data suggest that adenovirus can cause self-limiting gastroenteritis in dolphins. This adenoviral infection can be detected by serology and by PCR detection in fecal material. Lack of signs of hepatitis in sick dolphins may reflect restricted tissue tropism or virulence of this adenovirus compared to those of the adenovirus identified in sea lions. Gene sequence-based phylogenetic analysis supports a common origin of adenoviruses that affect sea mammals. Our findings suggest the need for vigilance against adenoviruses in captive and wild dolphin populations.




Rubio-Guerri C., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Crespo JL. and Sanchez-Vizcaino JM..




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Novel adenovirus detected in captive bottlenose dolphins (Tursiops truncatus) suffering from self-limiting gastroenteritis

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Novel adenovirus detected in captive bottlenose dolphins (Tursiops truncatus) suffering from self-limiting gastroenteritis

Participants:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Generalitat ValencianaOceanogràfic. Ciudad de las Artes y las Ciencias. Generalitat Valenciana.







BMC Veterinary Research
FACTOR YEAR Q
1.643 2015

NLMID: 101249759

PMID: 25888777

ISSN: 1746-6148



TITLE: Novel adenovirus detected in captive bottlenose dolphins (Tursiops truncatus) suffering from self-limiting gastroenteritis


JOURNAL: BMC Vet. Res


NUMERACIÓN: 11(53):1-7


AÑO: 2015


PUBLISHER: BioMed Central


AUTHORS: Rubio-Guerri C., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Crespo JL. and Sanchez-Vizcaino JM..


VISAVET PARTICIPANTS


4th
Mar Melero Asensio
Last
José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/ 10.1186/s12917-015-0367-z


CITE THIS PUBLICATION:

Rubio-Guerri C., Garcia-Parraga D., Nieto-Pelegrin E., Melero M., Alvaro T., Valls M., Crespo JL. and Sanchez-Vizcaino JM. Novel adenovirus detected in captive bottlenose dolphins (Tursiops truncatus) suffering from self-limiting gastroenteritis. BMC Veterinary Research. 11(53):1-7. 2015. (A). ISSN: 1746-6148. DOI: 10.1186/s12917-015-0367-z


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