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Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes

Investigation article published in Veterinary Microbiology

April 9th, 2018

This study investigated the genetic characteristics of 121 ovine Mannheimia haemolytica isolates from lungs with (n = 75) and without pneumonic lesions (n = 46) using multilocus sequence typing (MLST), virulence-associated gene typing and pulsed-field gel electrophoresis (PFGE). Twelve STs were identified with most isolates (81%) belonged to ST16, ST28 and ST8. Analysis of the M. haemolytica MLST Database indicate a wide distribution of these genotypes in small ruminants, never reported in bovine isolates. This could suggest the adaptation of certain genetic lineages of M. haemolytica to small ruminants. e-BURST analysis grouped most STs into three clonal complexes (CC2, CC8 and CC28), consistent with a clonal population structure of M. haemolytica. Virulence-associated gene typing identified five virulence profiles in 64% and 65.1% of the M. haemolytica isolates from lungs with and without pneumonic lesions, respectively. These data suggest that M. haemolytica isolates from the lungs with and without pneumonic lesions are genetically homogeneous. By PGFE analysis a high level of genetic diversity was observed not only within isolates from lungs without pneumonic lesions but also among isolates from pneumonic lesions (GD 0.69 and GD 0.66, respectively; P > 0.05). These results indicate that multiple strains of M. haemolytica may be associated with individual cases of pneumonia in sheep




Garcia-Alvarez A., Fernandez-Garayzabal JF., Chaves F., Pinto C. and Cid D.




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Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes

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Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes


Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Comunidad de MadridDepartamento de Microbiología Clínica. Hospital Universitario 12 de Octubre. Salud Madrid. Comunidad de Madrid.

Universidad Nacional Mayor de San Marcos.







Veterinary Microbiology
FACTOR YEAR Q
2.791 2018

NLMID: 7705469

PMID: 29778209

ISSN: 0378-1135



TITLE: Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 219:80-86


AÑO: 2018


PUBLISHER: Elsevier Science Publishers


AUTHORS: Garcia-Alvarez A., Fernandez-Garayzabal JF., Chaves F., Pinto C. and Cid D.


VISAVET PARTICIPANTS


José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/ 10.1016/j.vetmic.2018.04.012


CITE THIS PUBLICATION:

Garcia-Alvarez A., Fernandez-Garayzabal JF., Chaves F., Pinto C. and Cid D. Ovine Mannheimia haemolytica isolates from lungs with and without pneumonic lesions belong to similar genotypes. Veterinary Microbiology. 219:80-86. 2018. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2018.04.012


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