Investigation article published in Journal of Clinical Microbiology

October 1^st, 2007

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism

Castellanos E., Aranaz A., Romero B., de Juan L., Alvarez J., Bezos J., Rodriguez-Campos S., Stevenson K., Mateos A. and Dominguez L..

Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Moredun Research Institute.
	Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	MAEVA SERVET, S.L..