Home \ Research \ Scientific publications \


Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

Investigation article published in Journal of Clinical Microbiology

October 1st, 2007

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism




Castellanos E., Aranaz A., Romero B., de Juan L., Alvarez J., Bezos J., Rodriguez-Campos S., Stevenson K., Mateos A. and Dominguez L..




See this article
Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

See it on NLM PubMed
Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Moredun Research Institute.

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

MAEVA SERVET, S.L.MAEVA SERVET, S.L..







Journal of Clinical Microbiology
FACTOR YEAR Q
3.708 2007

NLMID: 7505564

PMID: 17670925

ISSN: 0095-1137



TITLE: Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates


JOURNAL: J. Clin. Microbiol.


NUMERACIÓN: 45(10):3439-42


AÑO: 2007


PUBLISHER: American Society for Microbiology


AUTHORS: Castellanos E., Aranaz A., Romero B., de Juan L., Alvarez J., Bezos J., Rodriguez-Campos S., Stevenson K., Mateos A. and Dominguez L..


VISAVET PARTICIPANTS


3rd
Beatriz Romero Martínez
4th
Lucía de Juan Ferré
5th
Julio Álvarez Sánchez
6th
Javier Bezos Garrido
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1128/JCM.01411-07


CITE THIS PUBLICATION:

Castellanos E., Aranaz A., Romero B., de Juan L., Alvarez J., Bezos J., Rodriguez-Campos S., Stevenson K., Mateos A. and Dominguez L. Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates. Journal of Clinical Microbiology. 45(10):3439-42. 2007. (A). ISSN: 0095-1137. DOI: 10.1128/JCM.01411-07


UNITS: