Molecular characterization of human reservoirs potentially pathogenic Escherichia coli, animal and environmental
Characterization of potentially pathogenic E. coli samples from different origins.
Objectives:
- Detection of virulence genes using quantitative real time PCR to distinguish the samples intended for isolation of a positive strain.
- Studies of antibiotic resistance in strains from healthy animals.
- Assess the genetic diversity of STEC isolates by pulsed field gel electrophoresis (PFGE) and Next Generation Sequencing (NGS).
- Detection serogroups / serotypes related-genes associated with hemolytic uremic syndrome (HUS).
- Shigatoxin producing E. coli prevalence studies in healthy animals.
Start date: June 1st, 2012
Heads of the research line:
Domínguez Rodríguez, Lucas
Ugarte Ruiz, María
VISAVET unit involved:
• Foodborne Zoonoses and Antimicrobial Resistance Unit (ZTA)
