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Molecular characterization of human reservoirs potentially pathogenic Escherichia coli, animal and environmental

Characterization of potentially pathogenic E. coli samples from different origins.

Objectives:

  • Detection of virulence genes using quantitative real time PCR to distinguish the samples intended for isolation of a positive strain.
  • Studies of antibiotic resistance in strains from healthy animals.
  • Assess the genetic diversity of STEC isolates by pulsed field gel electrophoresis (PFGE) and Next Generation Sequencing (NGS).
  • Detection serogroups / serotypes related-genes associated with hemolytic uremic syndrome (HUS).
  • Shigatoxin producing E. coli prevalence studies in healthy animals.

 

Start date: June 1st, 2012

Heads of the research line:
Domínguez Rodríguez, Lucas
Ugarte Ruiz, María

VISAVET unit involved:

•  Foodborne Zoonoses and Antimicrobial Resistance Unit (ZTA)




Last publications resulting
from the research line