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The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats

Veterinary Immunology and Immunopathology publish this investigation article

May 1st, 2018

The diagnosis of tuberculosis (TB) in goats is based mainly on the single and comparative intradermal tuberculin (SIT and CIT) tests and, exceptionally, on the interferon-gamma (IFN-γ) assay, however they are not perfect in terms of sensitivity and specificity. Nevertheless, various serological assays that provide a potential cost-effective approach for the control of TB are also available or under development, and a variety of results have been reported regarding the ability of these tests to detect infected animals, particularly in the early stages of infection.
In the present study, SIT/CIT and IFN-γ tests and three different serological assays were evaluated during two consecutive herd testing events in a recently infected caprine herd (n = 447) with a high prevalence of infection in order to evaluate their performance and provide field data with which to improve the TB control programs in this species. The proportion of infected animals that tested positive among all the infected goats (T+/I+ value) in the last herd testing event ranged from 26.2% (IC95%; 19.3–34.5) to 85.7% (IC95%; 78.5–90.7) using cell-based diagnostic tests. The SIT/SCIT tests detected more infected goats than the IFN-γ test, regardless of the interpretation criteria. The T+/I+ value of serology was 83.2 (IC95%; 75.2–89), although it increased significantly (P < 0.05) when using samples collected 15 days after the intradermal test (100%, IC95%; 97–100). In general, a parallel interpretation of intradermal tests with serology maximized the detection of infected goats. These results demonstrate that serological tests are valuable diagnostic tools to maximize the detection of TB infected goats, even in recent outbreaks, accelerating the eradication process




Bezos J., Roy A., Infantes-Lorenzo JA., Gonzalez I., Venteo A., Romero B., Grau A., Minguez O., Dominguez L. and de Juan L..




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The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats

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The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats


Participants:

Universidad ComplutenseServicio de Ensayos Clínicos (SEC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

CZ Veterinaria, S.A.CZ Veterinaria, S.A. (CZV).

Inmunología y Genética Aplicada, S.A.Inmunología y Genética Aplicada, S.A. (INGENASA).

MAEVA SERVET, S.L.MAEVA SERVET, S.L..

Junta de Castilla y LeónServicio de Sanidad Animal. Junta de Castilla y León.







Veterinary Immunology and Immunopathology
FACTOR YEAR Q
1.846 2018

NLMID: 8002006

PMID: 29678229

ISSN: 0165-2427



TITLE: The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats


JOURNAL: Vet. Immunol. Immunopathol.


NUMERACIÓN: 199:43-52


AÑO: 2018


PUBLISHER: Elsevier Scientific


AUTHORS: Bezos J., Roy A., Infantes-Lorenzo JA., Gonzalez I., Venteo A., Romero B., Grau A., Minguez O., Dominguez L. and de Juan L..


VISAVET PARTICIPANTS


First
Javier Bezos Garrido
2nd
Álvaro Roy Cordero
6th
Beatriz Romero Martínez
9th
Lucas Domínguez Rodríguez
Last
Lucía de Juan Ferré

DOI: https://doi.org/ 10.1016/j.vetimm.2018.03.006


CITE THIS PUBLICATION:

Bezos J., Roy A., Infantes-Lorenzo JA., Gonzalez I., Venteo A., Romero B., Grau A., Minguez O., Dominguez L. and de Juan L. The use of serological tests in combination with the intradermal tuberculin test maximizes the detection of tuberculosis infected goats. Veterinary Immunology and Immunopathology. 199:43-52. 2018. (A). ISSN: 0165-2427. DOI: 10.1016/j.vetimm.2018.03.006


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