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Virulence - Associated Genes and Antimicrobial Resistance in Shigatoxin-producing Escherichia coli isolates from healthy Spanish cattle

Poster communication in WVA/WMA Global Conference on One Health

May 21st, 2015

Cabal A., Porrero MC., de la Cruz ML., Barcena C., Dominguez L. and Alvarez J.

Escherichia coli is a commensal bacteria, but some strains may be pathogenic for humans. Those with pathogenic potential are known as pathotypes. Six pathotypes have been described until now, being by far, the Shigatoxin-producing Escherichia coli (STEC) the most studied one. Ruminants (especially cattle), are considered the most important reservoir of STEC for humans through the consumption of their undercooked meat. Several outbreaks have been attributed specifically to O157:H7 serotype, hence its importance for the Public Health. However, non-O157 serogroups have become relevant in the last years as for example the O104:H4 STEC/EAEC hybrid strains. Antimicrobial resistance (AMR) among STEC strains is also an issue of concern due to the increase of resistances in the last years. The aim of this study was to identify the Virulence Associated Genes (VAGs), the AMR and the Pulsed Field Gel Electrophoresis (PFGE) patterns in a collection of STEC isolates obtained from the feces or hair of healthy cattle in Spain in 2011 (feces) and 2013 (hair). Sixty-four STEC isolates were recovered (prevalence of 10.67 and 15.44% in 2011 and 2013) and investigated by real-time PCR for the presence of nine VAGs belonging to five E. coli pathotypes (stx1, sxt2, eae, ehxA, aggR, invA, bfpA, est, and elt) and ten serogroup-related genes related to hemolytic uremic syndrome (HUS). In addition, isolates were tested for twelve antimicrobials by broth microdilution and their genetic distance was assessed using PFGE. EhxA, stx2, eae, stx1 and est were the most frequently detected VAGs. BfpA was detected in one isolate and no positive isolates were found for aggR, elt and invA. 12.5% of the STEC isolates were O157:H7 whereas O26, O111, O91, O145 and O104 were detected only once or twice. Only one O104 positive isolate (serotype O104:H7) was found. 41% STEC/ETEC hybrid strains were detected among the non-O157 isolates. Regarding the AMR, 62.5% of the isolates presented resistances to at least one antimicrobial being the most prevalent resistances for sulfamethoxazole, streptomycin, tetracyclin and trimethoprim. No significant differences were found between O157 and non-O157 isolates. A maximum of 80% similarity among PFGE patterns was observed




Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidad de Castilla La ManchaUniversidad de Castilla La Mancha (UCLM).

University of MinnesotaDepartment of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota (UMM).

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.


Link to WVA/WMA Global Conference on One Health





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WVA/WMA Global Conference on One Health


May 21st-22nd, 2015
Madrid
Spain

TITLE: Virulence - Associated Genes and Antimicrobial Resistance in Shigatoxin-producing Escherichia coli isolates from healthy Spanish cattle


TYPE: Poster communication


AUTHORS: Cabal A., Porrero MC., de la Cruz ML., Barcena C., Dominguez L. and Alvarez J.


VISAVET PARTICIPANTS


4th
Carmen Bárcena Asensio
5th
Lucas Domínguez Rodríguez
Last
Julio Álvarez Sánchez

DATE: May 21st, 2015


CITE THIS COMMUNICATION:

Cabal A., Porrero MC., de la Cruz ML., Barcena C., Dominguez L. and Alvarez J. Virulence - Associated Genes and Antimicrobial Resistance in Shigatoxin-producing Escherichia coli isolates from healthy Spanish cattle. WVA/WMA Global Conference on One Health, World Veterinary Association, World Medical Association, Madrid, Spain, May 21st, 2015. (Poster communication)


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