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A naturally occurring SNP in plasmid pB1000 produces a reversible increase in antibiotic resistance


24 de enero de 2017

Investigación publicada en Antimicrobial Agents and Chemotherapy


ColE1 plasmids are small mobilizable replicons that play an important role in the spread of antibiotic resistance in Pasteurellaceae. In this study we describe how a natural single nucleotide polymorphism (SNP) near the origin of replication of the ColE1-type plasmid pB1000 found in a Pasteurella multocida clinical isolate, generates two independent plasmid variants able to coexist in the same cell simultaneously. Using Haemophilus influenzae Rd strain as a model system, we combined antibiotic susceptibility tests, quantitative PCRs, competition assays and experimental evolution to characterize the consequences of the coexistence of the pB1000 plasmid variants. This coexistence produced an increase of the total plasmid copy number (PCN) in the host bacteria, leading to a rise in both the antibiotic resistance level and the metabolic burden produced by pB1000. Using experimental evolution we showed that in the presence of ampicillin the bacteria maintained both plasmid variants for 300 generations. In the absence of antibiotics, on the other hand, the bacteria are capable of reverting to the single-plasmid genotype via the loss of one of the plasmid variants. Our results revealed how a single mutation in plasmid pB1000 provides the bacterial host with a mechanism to increase PCN and consequently ampicillin resistance level. Crucially, this mechanism can be rapidly reversed to avoid the extra cost entailed by the increased PCN in the absence of antibiotics




Santos-Lopez A., Bernabe-Balas C., Ares M., Ortega-Huedo R., Hoefer A., San Millan A. y Gonzalez-Zorn B..




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A naturally occurring SNP in plasmid pB1000 produces a reversible increase in antibiotic resistance

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A naturally occurring SNP in plasmid pB1000 produces a reversible increase in antibiotic resistance

Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Antimicrobial Agents and Chemotherapy
FACTOR YEAR Q
4.302 2016

NLMID: 315061

PMID: 27895020

ISSN: 0066-4804



TÍTULO: A naturally occurring SNP in plasmid pB1000 produces a reversible increase in antibiotic resistance


REVISTA: Antimicrob. Agents Chemother.


NUMERACIÓN: 61(2). pii: e01735-16


AÑO: 2017


EDITORIAL: American Society for Microbiology


AUTORES: Santos-Lopez A., Bernabe-Balas C., Ares M., Ortega-Huedo R., Hoefer A., San Millan A. and Gonzalez-Zorn B..


PARTICIPANTES VISAVET


2nd
Cristina Bernabé Balas
3rd
Manuel Ares Arroyo
5th
Andreas Hoefer
Last
Bruno González Zorn

DOI: https://doi.org/10.1128/AAC.01735-16


CITA ESTA PUBLICACIÓN:

Santos-Lopez A., Bernabe-Balas C., Ares M., Ortega-Huedo R., Hoefer A., San Millan A. y Gonzalez-Zorn B. A naturally occurring SNP in plasmid pB1000 produces a reversible increase in antibiotic resistance. Antimicrobial Agents and Chemotherapy. 61(2). pii: e01735-16. 2017. (A). ISSN: 0066-4804. DOI: 10.1128/AAC.01735-16


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