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Detection and characterization of extended-spectrum beta-lactamases in Salmonella enterica strains of healthy food animals in Spain

The Journal of Antimicrobial Chemotherapy publica este artículo de investigación

1 de octubre de 2006

OBJECTIVES: To carry out the characterization of the genes encoding extended-spectrum beta-lactamases (ESBLs) and their genetic environments in four expanded-spectrum cephalosporin-resistant Salmonella enterica isolates (serovars: two Virchow, one Enteritidis, one Rissen) recovered during the monitoring programmes performed in Spain by the VAV Network from faecal samples of pigs, poultry and laying hens at the slaughterhouse level. METHODS: The presence and characterization of ESBL genes as well as their genetic environments in the four S. enterica isolates were investigated by PCR and sequencing. The presence of other resistance genes was also analysed by PCR and sequencing. RESULTS: Three avian S. enterica isolates (two Virchow and one Enteritidis) harboured the bla(CTX-M-9) gene combined with bla(TEM-1b). The bla(CTX-M-9) gene was included in these three isolates in a class 1 integron with the following 5`-->3` structure: integron 1 variable region (dfrA16-aadA2 gene cassettes)-qacEDelta1-sul1-orf513-bla(CTX-M-9)-orf3-like-orf1005. The sul2 gene was also detected in these three bla(CTX-M-9)-containing isolates and tet(A) in one of them. The two serovar Virchow isolates showed an indistinguishable PFGE pattern, although they were recovered from different animal species (broiler and laying hen). A porcine ESBL-positive isolate (serovar Rissen) harboured the bla(SHV-12) gene combined with bla(TEM-1b). This bla(SHV-12)-containing isolate also harboured the tet(A), aadA and sul1 genes. CONCLUSIONS: The emergence of ESBL-producing S. enterica isolates among food animals is described for the first time in Spain, with those of the CTX-M group being the predominant ESBLs detected




Riano I., Moreno MA., Teshager T., Dominguez L. y Torres C.




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Detection and characterization of extended-spectrum beta-lactamases in Salmonella enterica strains of healthy food animals in Spain

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Detection and characterization of extended-spectrum beta-lactamases in Salmonella enterica strains of healthy food animals in Spain


Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad de La RiojaÁrea de Bioquímica y Biología Molecular. Universidad de La Rioja (UR).







The Journal of Antimicrobial Chemotherapy
FACTOR YEAR Q
3.891 2006

NLMID: 7513617

PMID: 16935865

ISSN: 0305-7453



TÍTULO: Detection and characterization of extended-spectrum beta-lactamases in Salmonella enterica strains of healthy food animals in Spain


REVISTA: J. Antimicrob. Chemother.


NUMERACIÓN: 58(4):844-7


AÑO: 2006


EDITORIAL: Oxford University Press


AUTORES: Riano I., Moreno MA., Teshager T., Dominguez L. and Torres C.


PARTICIPANTES VISAVET


2nd
Miguel Ángel Moreno Romo
4th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1093/jac/dkl337


CITA ESTA PUBLICACIÓN:

Riano I., Moreno MA., Teshager T., Dominguez L. y Torres C. Detection and characterization of extended-spectrum beta-lactamases in Salmonella enterica strains of healthy food animals in Spain. The Journal of Antimicrobial Chemotherapy. 58(4):844-7. 2006. (A). ISSN: 0305-7453. DOI: 10.1093/jac/dkl337


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