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Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar

Investigación publicada en Pathogens

25 de marzo de 2020

The presence of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex (MTC) is a main concern in wildlife populations such as the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against the MTC are valuable for tuberculosis (TB) monitoring and control and particularly useful in suids. The development of accurate, efficient, and non-invasive new tools to detect exposure to MTC would be highly beneficial for improving disease surveillance. This study aimed to determine if antibodies against MTC could be detected in oral fluid (OF) samples by a new ELISA test (IgG detection) from naturally TB-infected wild boar. For this, individual, paired serum and OF samples were collected from 148 live wild boar in two TB-status areas from Spain and quantitatively used to validate the new ELISA test. Antibodies against MTC were widely detected in OF samples, for which a significant positive correlation (r = 0.83) was found with the validated serology test. OF ELISA sensitivity and specificity were 67.3% and 100%, respectively. The results of this work suggest that OF samples have the potential to be used for MTC diagnosis as a further step in TB surveillance and control in suid populations. Based on our results, further research is warranted and could be performed using non-invasive new tools directly in field conditions to detect exposure to MTC




Barasona JA., Barroso-Arevalo S., Rivera B., Gortazar C. y Sanchez-Vizcaino JM..




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Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar

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Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar


Participantes:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).







Pathogens
FACTOR YEAR Q
3.405 2018

NLMID: 101596317

PMID: 32218183

ISSN: 2076-0817



TÍTULO: Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar


REVISTA: Pathogens


NUMERACIÓN: 9(4), 242


AÑO: 2020


EDITORIAL: MDPI


AUTORES: Barasona JA., Barroso-Arevalo S., Rivera B., Gortazar C. and Sanchez-Vizcaino JM..


PARTICIPANTES VISAVET


First
José Ángel Barasona García-Arévalo
2nd
Sandra Barroso Arévalo
3rd
Belén Rivera Arroyo
Last
José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/10.3390/pathogens9040242


CITA ESTA PUBLICACIÓN:

Barasona JA., Barroso-Arevalo S., Rivera B., Gortazar C. y Sanchez-Vizcaino JM. Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar. Pathogens. 9(4), 242. 2020. (A). ISSN: 2076-0817. DOI: 10.3390/pathogens9040242


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