Inicio \ Investigación \ Publicaciones científicas \


Development and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas

Artículo de investigación publicado en Frontiers in veterinary science

13 de agosto de 2018

South American camelids are susceptible to tuberculosis, caused mainly by Mycobacterium bovis and M. microti. Despite the tuberculin skin test being the official test for tuberculosis, it has a very low sensitivity in these species (14–20%). Serological tests present the advantages of being rapid, easy to perform and facilitate analysis of large numbers of samples in a short period of time. Novel antigen discovery and evaluation would provide enhanced detection of specific antibodies against members of M. tuberculosis complex. Here, we describe the development and evaluation of an ELISA-type immunoassays to use in the diagnosis of tuberculosis in llamas and alpacas based on P22, a multiprotein complex obtained by affinity chromatography from bovine Purified Protein Derivative (bPPD), that showed high sensitivity and specificity in mice, cattle and goats. This work was performed in two stages. First, a preliminary panel of samples collected from tuberculosis-free (n = 396) and M. bovis-infected herds (n = 56) was assayed, obtaining high specificity (100%) and sensitivity ranging from 63 to 96%. Subsequently, the use of the serological assay was tested using samples from two herds suffering from clinical M. bovis (n = 88) and M. microti (n = 25) infection to evaluate the ability of the ELISA to detect infected animals. 11 out of 88 alpacas were positive to the ELISA in a M. bovis outbreak and 7 out of 25 in a M. microti outbreak. The P22 ELISA potentially provides a sensitive and specific platform for improved tuberculosis surveillance in camelids




Infantes-Lorenzo JA., Whiyehead CE., Moreno I., Bezos J., Roy A., Dominguez L., Dominguez M. y Salguero FJ.




Ver artículo
Development and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas

Ver en NLM PubMed
Development and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas



Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

University of SurreySchool of Veterinary Medicine. Faculty of Health and Medical Sciences. University of Surrey.

Camelid Veterinary Services Ltd.

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Frontiers in veterinary science
FACTOR YEAR Q
1.036 2018

NLMID: 101666658

PMID: 30151368

ISSN: 2297-1769



TÍTULO: Development and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas


REVISTA: Front Vet Sci


NUMERACIÓN: 5(189):1-7


AÑO: 2018


EDITORIAL: Lausanne : Frontiers Media S.A


AUTORES: Infantes-Lorenzo JA., Whiyehead CE., Moreno I., Bezos J., Roy A., Dominguez L., Dominguez M. and Salguero FJ.


PARTICIPANTES VISAVET


4th
Javier Bezos Garrido
6th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.3389/fvets.2018.00189


CITA ESTA PUBLICACIÓN:

Infantes-Lorenzo JA., Whiyehead CE., Moreno I., Bezos J., Roy A., Dominguez L., Dominguez M. y Salguero FJ. Development and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas. Frontiers in veterinary science. 5(189):1-7. 2018. (A). ISSN: 2297-1769. DOI: 10.3389/fvets.2018.00189


SERVICIOS: