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Effect of the topical administration of corticosteroids and tuberculin pre-sensitisation on the diagnosis of tuberculosis in goats

BMC Veterinary Research publica este artículo de investigación

27 de enero de 2022

Background: Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB control and eradication programmes have traditionally been based on intradermal tuberculin tests and slaughterhouse surveillance. However, this strategy has limitations in terms of sensitivity and specificity. Different factors may affect the performance of the TB diagnostic tests used in goats and, subsequently, the detection of TB-infected animals. In the present study, the effect of two of the factors that may affect the performance of the techniques used to diagnose TB in goats, the topical administration of corticosteroids and a recent pre-sensitisation with tuberculin, was analysed.
Methods: The animals (n = 151) were distributed into three groups: (1) a group topically treated with corticosteroids 48 h after intradermal tuberculin tests (n = 53); (2) a group pre-sensitised with bovine and avian purified protein derivatives (PPDs) 3 days before the intradermal tuberculin test used for TB diagnosis (n = 48); and (3) a control group (n = 50). All the animals were tested using single and comparative intradermal tuberculin (SIT and CIT, respectively) tests, an interferon-gamma release assay (IGRA) and a P22 ELISA.
Results: The number of SIT test reactors was significantly lower in the group treated with corticosteroids when compared to the pre-sensitised (p < 0.001) and control (p = 0.036) groups. In contrast, pre-sensitisation with bovine and avian PPDs did not cause a significant reduction in the number of SIT and CIT test reactors compared with the control group. In fact, a higher number of reactors was observed after the prior tuberculin injection in the pre-sensitised group (p > 0.05). No significant effect was observed on IGRA and P22 ELISA due to corticosteroids administration. Nevertheless, a previous PPD injection affected the IGRA performance in some groups.
Conclusions: The application of topical corticosteroid 24 h before reading the SIT and CIT tests can reduce the increase in skin fold thickness and subsequently significantly decrease the number of positive reactors. Corticosteroids used can be detected in hair samples. A previous pre-sensitisation with bovine and avian PPDs does not lead to a significant reduction in the number of intradermal tests reactors. These results are valuable in order to improve diagnosis of caprine TB and detect fraudulent activities in the context of eradication programs




Ortega J., Roy A., Díaz-Castillo A., de Juan L., Romero B., Saez-Llorente JL., Dominguez L., Regal P., Infantes-Lorenzo JA., Alvarez J. y Bezos J..




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Effect of the topical administration of corticosteroids and tuberculin pre-sensitisation on the diagnosis of tuberculosis in goats

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Effect of the topical administration of corticosteroids and tuberculin pre-sensitisation on the diagnosis of tuberculosis in goats



Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Ministerio de Agricultura, Pesca y AlimentaciónMinisterio de Agricultura, Pesca y Alimentación (MAPA).

Universidad de Santiago de CompostelaDepartamento de Química Analítica, Nutrición y Bromatología. Facultad de Veterinaria. Universidad de Santiago de Compostela (USC).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Ensayos Clínicos (SEC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







BMC Veterinary Research
FACTOR YEAR Q
2.741 2020

NLMID: 101249759

PMID: 35086534

ISSN: 1746-6148



TÍTULO: Effect of the topical administration of corticosteroids and tuberculin pre-sensitisation on the diagnosis of tuberculosis in goats


REVISTA: BMC Vet. Res


NUMERACIÓN: 18(1):58


AÑO: 2022


EDITORIAL: BioMed Central


AUTORES: Ortega J., Roy A., Díaz-Castillo A., de Juan L., Romero B., Saez-Llorente JL., Dominguez L., Regal P., Infantes-Lorenzo JA., Alvarez J. and Bezos J..


PARTICIPANTES VISAVET


First
Javier Ortega Martín
4th
Lucía de Juan Ferré
5th
Beatriz Romero Martínez
7th
Lucas Domínguez Rodríguez
10th
Julio Álvarez Sánchez
Last
Javier Bezos Garrido

DOI: https://doi.org/10.1186/s12917-022-03156-0


CITA ESTA PUBLICACIÓN:

Ortega J., Roy A., Díaz-Castillo A., de Juan L., Romero B., Saez-Llorente JL., Dominguez L., Regal P., Infantes-Lorenzo JA., Alvarez J. y Bezos J. Effect of the topical administration of corticosteroids and tuberculin pre-sensitisation on the diagnosis of tuberculosis in goats. BMC Veterinary Research. 18(1):58. 2022. (A). ISSN: 1746-6148. DOI: 10.1186/s12917-022-03156-0


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