Inicio \ Investigación \ Publicaciones científicas \


Genome comparison of erythromycin resistant Campylobacter from turkey identifies hosts and pathways for horizontal spread of erm(B) genes


15 de noviembre de 2017

Artículo de investigación publicado en Frontiers in Microbiology


Pathogens in the genus Campylobacter are the most common cause of food-borne bacterial gastro-enteritis. Campylobacteriosis, caused principally by Campylobacter jejuni and C. coli, is transmitted to humans by food of animal origin, especially poultry. As for many pathogens, antimicrobial resistance in Campylobacter is increasing at an alarming rate. Erythromycin prescription is the treatment of choice for clinical cases requiring antimicrobial therapy but this is compromised by mobility of the erythromycin resistance gene erm(B) between strains. Here we evaluate resistance to six antimicrobials in 170 Campylobacter isolates (133 C. coli and 37 C. jejuni) from turkeys. Erythromycin resistant isolates (n=85; 81 C. coli and 4 C. jejuni) were screened for the presence of the erm(B) gene, that has not previously been identified in isolates from turkeys. The genomes of 2 positive C. coli isolates were sequenced and in both isolates the erm(B) gene clustered with resistance determinants against aminoglycosides plus tetracycline, including aad9, aadE, aph(2")-IIIa, aph(3´)-IIIa and tet(O) genes. Comparative genomic analysis identified identical erm(B) sequences among Campylobacter from turkeys, Streptococcus suis from pigs and Enterococcus faecium and Clostridium difficile from humans. This is consistent with multiple horizontal transfer events among different bacterial species colonizing Turkeys. This example highlights the potential for dissemination of antimicrobial resistance across bacterial species boundaries which may compromise their effectiveness in antimicrobial therapy




Florez-Cuadrado D., Ugarte-Ruiz M., Meric G., Quesada A., Porrero MC., Pascoe B., Saez Llorente JL., Lopez-Orozco G., Dominguez L. y Sheppard S.




Ver artículo
Genome comparison of erythromycin resistant Campylobacter from turkey identifies hosts and pathways for horizontal spread of erm(B) genes



Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

The Milner Centre for Evolution. Department of Biology and Biochemistry. University of Bath.

Universidad de ExtremaduraDepartamento de Bioquímica, Biología Molecular y Genética. Facultad de Veterinaria. Universidad de Extremadura (UNEX).

Subdirección General de Sanidad de la Producción Primaria. Dirección General de Recursos Agrícolas y Ganaderos.

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Frontiers in Microbiology
FACTOR YEAR Q
4.076 2016

NLMID: 101548977

ISSN: 1664-302X



TÍTULO: Genome comparison of erythromycin resistant Campylobacter from turkey identifies hosts and pathways for horizontal spread of erm(B) genes


REVISTA: Front Microbiol


NUMERACIÓN: 2240(8):1-8


AÑO: 2017


EDITORIAL: Frontiers Research Foundation


AUTORES: Florez-Cuadrado D., Ugarte-Ruiz M., Meric G., Quesada A., Porrero MC., Pascoe B., Saez Llorente JL., Lopez-Orozco G., Dominguez L. and Sheppard S.


PARTICIPANTES VISAVET


First
Diego Flórez Cuadrado
2nd
María Ugarte Ruiz
9th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.3389/fmicb.2017.02240


CITA ESTA PUBLICACIÓN:

Florez-Cuadrado D., Ugarte-Ruiz M., Meric G., Quesada A., Porrero MC., Pascoe B., Saez Llorente JL., Lopez-Orozco G., Dominguez L. y Sheppard S. Genome comparison of erythromycin resistant Campylobacter from turkey identifies hosts and pathways for horizontal spread of erm(B) genes. Frontiers in Microbiology. 2240(8):1-8. 2017. (A). ISSN: 1664-302X. DOI: 10.3389/fmicb.2017.02240


SERVICIOS: