Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages
The Journal of Antimicrobial Chemotherapy publica este artículo de investigación
1 de noviembre de 2020
Objectives: To investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particles.
Methods: Several databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-level aminoglycoside resistance was used as a marker in eight different plasmids, from different incompatibility groups, and with differing sizes and plasmid copy numbers. All plasmids were transformed into Escherichia coli bearing one of four different lysogenic phages. Upon induction, encapsidation of armA in phage particles was evaluated using qRT-PCR and Southern blotting.
Results: Multicopy plasmids carry a vast set of emerging clinically important antimicrobial resistance genes. However, 60% of these plasmids do not bear mobility (MOB) genes. When carried on these multicopy plasmids, mobilization of a marker gene armA into phage capsids was up to 10 000 times more frequent than when it was encoded by a large plasmid with a low copy number.
Conclusions: Multicopy plasmids and phages, two major mobile genetic elements (MGE) in bacteria, represent a novel high-efficiency transmission route of antimicrobial resistance genes that deserves further investigation
Rodriguez-Campoo R., Serna-Bernaldo C., Ares M., Matamoros BR., Delgado-Blas JF., Montero N., Bernabe-Balas C., Wedel EF., Mendez IS., Muniesa M. y Gonzalez-Zorn B..
 | Departamento de Microbiología. Facultad de Biología. Universitat de Barcelona (UB). |
 | Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
| Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
