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Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)

Investigación publicada en Microorganisms

23 de marzo de 2020

In recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but, in many cases such as during hunting, an adequate serum sample cannot be obtained. In the present study, liver transudate was evaluated as an alternative matrix to serum for HEV detection. A total of 125 sera and liver transudates were tested by enzyme-linked immunosorbent assay at different dilutions (1:2, 1:10, 1:20), while 58 samples of serum and liver transudate were checked for the presence of HEV RNA by RT-qPCR. Anti- HEV antibodies were detected by ELISA in 68.0% of the serum samples, and in 61.6% of the undiluted transudate, and in 70.4%, 56.8%, and 44.8% of 1:2, 1:10, or 1:20 diluted transudate, respectively. The best results were obtained for the liver transudate at 1:10 dilution, based on the Kappa statistic (0.630) and intraclass correlation coefficient (0.841). HEV RNA was detected by RT-qPCR in 22.4% of the serum samples and 6.9% of the transudate samples, all samples used for RT-qPCR were positive by ELISA. Our results indicate that liver transudate may be an alternative matrix to serum for the detection of anti-HEV antibodies




Navarro A., Barcena C., Pozo P., Diez-Guerrier A., Martinez I., Polo Vaquero C., Duque C., Rodriguez-Lazaro D., Goyache J. y Garcia N..




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Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)

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Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)


Participantes:

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad de BurgosUnidad de Microbiología. Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos (UBU).







Microorganisms
FACTOR YEAR Q
4.142 2019

PMID: 32210090

ISSN: 2076-2607



TÍTULO: Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)


REVISTA: Microorganisms


NUMERACIÓN: 8(3):450


AÑO: 2020


EDITORIAL: MDPI AG


AUTORES: Navarro A., Barcena C., Pozo P., Diez-Guerrier A., Martinez I., Polo Vaquero C., Duque C., Rodriguez-Lazaro D., Goyache J. and Garcia N..


PARTICIPANTES VISAVET


First
Alejandro Navarro Gómez
2nd
Carmen Bárcena Asensio
4th
Alberto Antoine Díez Guerrier
5th
Irene Martínez Alares
7th
Clara Duque García-Aranda
9th
Joaquín Goyache Goñi
Last
Nerea García Benzaquén

DOI: https://doi.org/10.3390/microorganisms8030450


CITA ESTA PUBLICACIÓN:

Navarro A., Barcena C., Pozo P., Diez-Guerrier A., Martinez I., Polo Vaquero C., Duque C., Rodriguez-Lazaro D., Goyache J. y Garcia N. Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa). Microorganisms. 8(3):450. 2020. (A). ISSN: 2076-2607. DOI: 10.3390/microorganisms8030450


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