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Molecular detection of extended spectrum B-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria

PLoS ONE publica este artículo de investigación

3 de diciembre de 2020

The increase in antimicrobial resistance in developed and developing countries is a global public health challenge. In this context β-lactamase production is a major contributing factor to resistance globally. The aim of this study was to determine the prevalence of phenotypic and genotypic extended spectrum β-lactamases (ESBLs) in 296 E. coli isolates recovered from diarrhoeic children younger than five years in Kano whose susceptibility profile against 7 antimicrobials had been determined. The E. coli isolates were subjected to double disc synergy test for phenotypic ESBLs detection and ESBL associated genes (blaCTX-M, blaTEM and blaSHV) were detected using conventional PCR. Phenotypically, 12.8% (38/296) E. coli isolates presented a ESBLs phenotype, with a significantly higher proportion in isolates from females compared with males (P-value = 0.024). blaCTX-M 73.3% and blaTEM 73.3% were the predominant resistance genes in the ESBLs positive E. coli (each detected in 22/30 isolates, of which 14 harboured both). In addition, 1/30 harboured blaCTX-M + blaTEM + blaSHV genes simultaneously. This study demonstrates the presence of ESBLs E. coli isolates in clinically affected children in Kano, and demonstrates the circulation of blaCTX-M and blaTEM associated with those phenotypes. Enactment of laws on prudent antibiotic use is urgently needed in Kano




Saka, K.H., Garcia-Soto S., Dabo NT., Lopez-Chavarrias V., Muhammad B., Ugarte-Ruiz M. y Alvarez J..




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Molecular detection of extended spectrum B-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria

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Molecular detection of extended spectrum B-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria


Participantes:

Nigerian Stored Products Research InstituteNigerian Stored Products Research Institute (NSPRI).

Bayero University KanoDepartment of Microbiology. Bayero University Kano (BUK).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Bayero University KanoDepartment of Biological Sciences. Bayero University Kano (BUK).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







PLoS ONE
FACTOR YEAR Q
2.740 2019

NLMID: 101285081

PMID: 33270734

ISSN: 1932-6203



TÍTULO: Molecular detection of extended spectrum B-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria


REVISTA: PLoS ONE


NUMERACIÓN: 15(12):e0243130


AÑO: 2020


EDITORIAL: Public Library Science


AUTORES: Saka, K.H., Garcia-Soto S., Dabo NT., Lopez-Chavarrias V., Muhammad B., Ugarte-Ruiz M. and Alvarez J..


PARTICIPANTES VISAVET


4th
Vicente López Chavarrías
6th
María Ugarte Ruiz
Last
Julio Álvarez Sánchez

DOI: https://doi.org/10.1371/journal.pone.0243130


CITA ESTA PUBLICACIÓN:

Saka, K.H., Garcia-Soto S., Dabo NT., Lopez-Chavarrias V., Muhammad B., Ugarte-Ruiz M. y Alvarez J. Molecular detection of extended spectrum B-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria. PLoS ONE. 15(12):e0243130. 2020. (A). ISSN: 1932-6203. DOI: 10.1371/journal.pone.0243130


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