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Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks

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24 de diciembre de 2022

Studies that characterize bovine respiratory disease (BRD)-associated Pasteurella multocida isolates are scarce compared with research on isolates from other hosts and clinical backgrounds. In the present study, 170 P. multocida isolates from 125 BRD outbreaks were characterized by capsular and LPS typing as well as by virulotyping. Three capsular types (A, B, F) and three LPS genotypes (L2, L3, L6) were identified. Capsular and LPS typing revealed a very low genetic diversity (GD = 0.02) among P. multocida, with most isolates belonging to genotype A:L3 (97.6%). Virulotyping identified seven virulence-associated gene profiles, with two profiles including 95.9% of the isolates. A subset of isolates was further characterized by MLST and PFGE. The sequence types ST79 and ST13 were the most frequently identified and were grouped into the same clonal complex (CC13), a result that supports the clonal population structure of BRD-associated P. multocida isolates. PFGE typing also revealed a low genetic diversity (GD = 0.18), detecting a single pattern in 62.5% of the outbreaks in which multiple isolates were analyzed. Overall, 85.2% of the isolates belonged to pulsotypes with at least 80% genetic similarity, consistent with a clonal population structure observed by MLST analysis and corroborating the genetic relatedness of most P. multocida isolates associated with BRD in cattle




Calderón-Bernal JM., Fernandez A., Arnal JL., Sanz-Tejero C., Fernandez-Garayzabal JF., Vela AI. y Cid D.




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Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks

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Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks



Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Laboratorio ExopolLaboratorio Exopol.

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Animals
FACTOR YEAR Q
3.231 2021

NLMID: 101635614

PMID: 36611685

ISSN: 2076-2615



TÍTULO: Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks


REVISTA: Animals


NUMERACIÓN: 13(1):75


AÑO: 2022


EDITORIAL: MDPI


AUTORES: Calderón-Bernal JM., Fernandez A., Arnal JL., Sanz-Tejero C., Fernandez-Garayzabal JF., Vela AI. and Cid D.


PARTICIPANTES VISAVET


First
Johan Manuel Calderón Bernal
5th
José Francisco Fernández-Garayzabal Fernández
6th
Ana Isabel Vela Alonso

DOI: https://doi.org/10.3390/ani13010075


CITA ESTA PUBLICACIÓN:

Calderón-Bernal JM., Fernandez A., Arnal JL., Sanz-Tejero C., Fernandez-Garayzabal JF., Vela AI. y Cid D. Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks. Animals. 13(1):75. 2022. (A). ISSN: 2076-2615. DOI: 10.3390/ani13010075


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