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Novel gel-based and real-time PCR assays for the improved detection of African horse sickness virus

Investigación publicada en Journal of Virological Methods

1 de julio de 2008

In order to improve, ensure and accelerate the diagnosis of African horse sickness, a highly devastating, transboundary animal disease listed by the World Animal Health Organisation, (OIE) three novel diagnostic PCR assays were developed and tested in this study. The reverse transcription-PCR (RT-PCR) tests were the following: (a) a conventional, gel-based RT-PCR, (b) a real-time PCR with SYBR-Green-named rRT-PCR SYBR-Green-, and (c) a real-time PCR rRT-PCR with TaqMan probe (termed rRT-PCR TaqMan). The same pair of primers-directed against African Horse Sickness Virus (AHSV) segment 5, encoding the non-structural protein NS1, is used in the three tests listed above. The three PCR assays detected similarly the nine AHSV serotypes from cultivated viral suspensions of different origins. The RT-PCR assays provided high sensitivity ranging from 0.1 to 1.2TCID(50)/ml. The specificity was also high, considering that related viruses, such as Bluetongue virus, and other equine viruses, such as West Nile Virus, remained negative for RT-PCR amplification. The detection of AHSV virus can be completed within 2-3h. These results indicate that the novel PCR methods described in this paper provide robust and versatile tools that allow rapid and highly specific, simultaneous detection of all AHSV serotypes




Rodriguez B., Fernandez J., Sailleau C., Zientara S., Belak S., Arias M. y Sanchez-Vizcaino JM..




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Novel gel-based and real-time PCR assays for the improved detection of African horse sickness virus

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Novel gel-based and real-time PCR assays for the improved detection of African horse sickness virus



Participantes:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Centro de Investigación en Sanidad Animal (CISA). Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Ministerio de Ciencia e Innovación.


Swedish University of Agricultural Sciences (SLU).

National Veterinary InstituteDepartment of Virology, Immunobiology and Parasitology. National Veterinary Institute (SVA).







Journal of Virological Methods
FACTOR YEAR Q
2.077 2008

NLMID: 8005839

PMID: 18501973

ISSN: 0166-0934



TÍTULO: Novel gel-based and real-time PCR assays for the improved detection of African horse sickness virus


REVISTA: J Virol Methods


NUMERACIÓN: 151(1):87-94


AÑO: 2008


EDITORIAL: Elsevier/North-Holland Biomedical Press


AUTORES: Rodriguez B., Fernandez J., Sailleau C., Zientara S., Belak S., Arias M. and Sanchez-Vizcaino JM..


6th
María Luisa Arias Neira
Last
José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/10.1016/j.jviromet.2008.03.029


CITA ESTA PUBLICACIÓN:

Rodriguez B., Fernandez J., Sailleau C., Zientara S., Belak S., Arias M. y Sanchez-Vizcaino JM. Novel gel-based and real-time PCR assays for the improved detection of African horse sickness virus. Journal of Virological Methods. 151(1):87-94. 2008. (A). ISSN: 0166-0934. DOI: 10.1016/j.jviromet.2008.03.029


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