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Paratuberculosis and avian tuberculosis infections in one red deer farm studied by IS900 and IS901 RFLP analysis

Investigación publicada en Veterinary Microbiology

25 de febrero de 2005

As the attempt to eradicate paratuberculosis in one red deer (Cervus elaphus) farm failed, all 167 red deer of different age groups were slaughtered and examined by culture for mycobacteria, and the farm was closed down. Spleen and hepatic lymph nodes, mediastinal lymph node, ileocecal lymph node, and ileum were collected from each animal and examined (a total of 835 organs). Neither tuberculosis lesions nor pathognomic signs of paratuberculosis were detected. Among all microscopically negative for mycobacteria organs, Mycobacterium avium subsp. paratuberculosis alone was isolated from 165 organs, M. a. avium alone from 41 organs, and both pathogens from four organs. M. a. paratuberculosis alone was detected in 71 red deer, M. a. avium alone in 13 red deer and both pathogens in 18 red deer. Using standardised RFLP methods, three IS900 RFLP types B-C1, B-C16, and B-C32 were identified among 40 M. a. paratuberculosis isolates and four IS901 RFLP types N-B1, N-B3, N-B4, and P-B3 among 17 M. a. avium isolates




Machackova-Kopecna M., Bartos M., Straka M., Ludvik V., Svastova P., Alvarez J., Lamka J., Trcka I., Treml F., Parmova I. y Pavlik I.




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Paratuberculosis and avian tuberculosis infections in one red deer farm studied by IS900 and IS901 RFLP analysis

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Paratuberculosis and avian tuberculosis infections in one red deer farm studied by IS900 and IS901 RFLP analysis



Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Veterinary Research Institute. Czechoslovak Academy of Agricultural Sciences.

District Veterinary Administration.

Faculty of Pharmacy. Charles University (CU).

University of Veterinary and Pharmaceutical Sciences Brno (VFU).

State Veterinary Diagnostic Institute.







Veterinary Microbiology
FACTOR YEAR Q
2.175 2005

NLMID: 7705469

PMID: 15708824

ISSN: 0378-1135



TÍTULO: Paratuberculosis and avian tuberculosis infections in one red deer farm studied by IS900 and IS901 RFLP analysis


REVISTA: Vet Microbiol


NUMERACIÓN: 105(3-4):261-8


AÑO: 2005


EDITORIAL: Elsevier


AUTORES: Machackova-Kopecna M., Bartos M., Straka M., Ludvik V., Svastova P., Alvarez J., Lamka J., Trcka I., Treml F., Parmova I. and Pavlik I.


Julio Álvarez Sánchez

DOI: https://doi.org/10.1016/j.vetmic.2004.11.008


CITA ESTA PUBLICACIÓN:

Machackova-Kopecna M., Bartos M., Straka M., Ludvik V., Svastova P., Alvarez J., Lamka J., Trcka I., Treml F., Parmova I. y Pavlik I. Paratuberculosis and avian tuberculosis infections in one red deer farm studied by IS900 and IS901 RFLP analysis. Veterinary Microbiology. 105(3-4):261-8. 2005. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2004.11.008


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