Investigación publicada en Vaccine

7 de julio de 2014

A major cause of salmonellosis in humans is the contamination of pork products. Infection in pigs can be controlled using bio-security programs, but they are not sufficient in countries where a high level of infection is recorded. In this context, the use of vaccines can represent a valid supplementary method of control. Recently, we have demonstrated that an attenuated strain of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium ΔznuABC) is protective against systemic and enteric salmonellosis in mouse and pig infection models, candidating this strain as an oral attenuated vaccine. In this study, we compared the efficacy of this attenuated Salmonella Typhimurium strain when administered orally or parenterally. Furthermore, in order to reproduce a pseudo-natural infection model, vaccinated pigs were allocated in the same pen with animals shedding virulent Salmonella Typhimurium. Animals were monitored weekly after vaccination and contact with infected piglets. Diarrhea and ataxia were recorded and Salmonella shedding was tested individually through bacterial culture. After four weeks of cohousing, piglets were euthanized, after which lymph nodes reactivity and gross lesions of the gut sections were scored at necropsy. Organs were submitted to microbiological and histological analyses. The data reported herein show that parenterally vaccinated animals do not shed the attenuated strain, and at the same time the absence of symptoms and decrease in virulent strain shedding in feces from day 6 after challenge demonstrated protection against infection induced by virulent Salmonella Typhimurium. In conclusion, our findings suggest that this is an alternative route of Salmonella Typhimurium ΔznuABC administration, without ignoring the advantages associated with oral vaccination

Ruggeri J., Pesciarioli M., Gaetarelli B., Scaglione FE., Pregel P., Ammendola S., Battistoni A., Bollo E., Alborali GL. y Pasquali P.

Parenteral administration of attenuated Salmonella Typhimurium DeltaznuABC is protective against salmonellosis in piglets

Parenteral administration of attenuated Salmonella Typhimurium DeltaznuABC is protective against salmonellosis in piglets

	Istituto Zooprofilattico Sperimentale della Lombardia e dell Emilia Romagna (IZSLER).
	Department of Veterinary Public Health and Food Safety. Istituto Superiore di Sanità.
	FAO-Italia. Food and Agriculture Organization of the United Nations (FAO).
	Department of Biology. Università degli Studi di Roma Tor Vergata.
	Department of Veterinary Sciences. Università degli Studi di Torino (UNITO).
	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).