Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: a tool for studying epidemiology of tuberculosis
Artículo de investigación publicado en Journal of Clinical Microbiology
1 de noviembre de 1996
The spacer oligonucleotide typing (spoligotyping) method was evaluated for its ability to differentiate Mycobacterium bovis strains. This method detects the presence or absence of spacers of the direct repeat locus of the M. bovis genome. The spacers in the direct repeat locus are amplified by PCR and are detected by hybridization of the biotin-labelled PCR product with a membrane containing oligonucleotides derived from spacer sequences that have previously been bound to a membrane. One hundred eighty-two M. bovis isolates from domestic animals (cattle, goat, sheep, and cats) and wild animals (deer and wild boar) were spoligotyped, and the results were compared with those obtained by IS6110 restriction fragment length polymorphism analysis. Two rather homogeneous clusters of isolates containing 20 and 4 types, respectively, were identified by spoligotyping. The first cluster included isolates from cattle, cats, and feral animals. By spoligotyping, isolates from the Spanish wild boar and deer had the same pattern as some bovine isolates, suggesting transmission between these animals and cattle and highlighting the importance of the study of these reservoirs. The second cluster included all the caprine and ovine isolates. Within each cluster, the patterns of the different strains differed only slightly, suggesting that the spoligotypes may be characteristic of strains from particular animal species. Spoligotyping proved to be useful for studying the epidemiology of bovine M. bovis isolates, especially of those isolates containing only a single copy of IS6110. In view of our results, we suggest fingerprinting all M. bovis strains by the spoligotyping method initially and then by IS6110 restriction fragment length polymorphism typing of the strains belonging to the most common spoligotypes
Aranaz A., Liebana E., Mateos A., Dominguez L., Vidal D., Domingo M., Gonzalez-Llamazares O., Rodriguez-Ferri E., Bunschotten A., Van Emnden JDA. y Cousins DV.
 | Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
 | Departamento de Patología y Producciones Animales. Facultad de Veterinaria. Universitat Autònoma de Barcelona (UAB). |
 | Departamento de Patología Animal, Medicina Animal (Anatomía Patológica). Facultad de Veterinaria. Universidad de León. |
 | The Netherlands Tuberculosis Reference Laboratory. Centre for Infectious Disease Control. National Institute for Public Health and the Environment (RIVM). |
 | Laboratory for Bovine Tuberculosis. Animal Health Laboratories (AHL). Department of Agriculture and Food Western Australia (DAFWA). |
