Inicio \ Investigación \ Publicaciones científicas \


Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles

Investigación publicada en Journal of Veterinary Diagnostic Investigation

1 de noviembre de 2018

Staphylococcus aureus encompasses 2 subspecies ( aureus and anaerobius) with significant differences in their epidemiology and pathogenicity. We evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the rapid identification of both subspecies using a panel of 52 S. aureus isolates (30 subsp. anaerobius and 22 subsp. aureus) recovered from different origins, countries, and years. The on-board library identification system correctly identified 42 of 52 (81%) S. aureus isolates at the species level with score values >2.0. Limited performance was observed for differentiation of S. aureus subspecies (particularly subsp. anaerobius). Visual inspection of MALDI-TOF MS profiles identified 5 subspecies-specific mass peaks ( m/ z 3430 and 6861 in S. aureus subsp. anaerobius, and m/ z 4046, 6890, and 8093 in S. aureus subsp. aureus) with 100% sensitivity and specificity values, which is potentially useful for differentiating these subspecies. The suitability of 3 models, Genetic Analysis (GA), Quick Classifier (QC), and Supervised Neural Network, for automatic identification of both subspecies was evaluated using the Recognition Capability (RC) and Cross Validation (CV) values provided by the on-board ClinProTools software. The GA and QC models reached RC and CV values of 100%. Both models were externally validated using a panel of 26 S. aureus isolates of both subspecies, with both models correctly classifying all isolates of both subspecies. MALDI-TOF MS coupled with ClinProTools software represents a rapid and simple approach for S. aureus subspecies discrimination




Perez-Sancho M., Vela AI., Horcajo P., Ugarte-Ruiz M., Dominguez L., Fernandez-Garayzabal JF. y de la Fuente R.




Ver artículo
Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles

Ver en NLM PubMed
Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles

Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







FACTOR YEAR Q
1.219 2017

NLMID: 9011490

PMID: 30280650

ISSN: 1040-6387



TÍTULO: Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles


REVISTA: J Vet Diagn Invest


NUMERACIÓN: 30(6):813-820


AÑO: 2018


EDITORIAL: American Association Of Veterinary Laboratory Diagnosticians


AUTORES: Perez-Sancho M., Vela AI., Horcajo P., Ugarte-Ruiz M., Dominguez L., Fernandez-Garayzabal JF. and de la Fuente R.


PARTICIPANTES VISAVET


First
Marta Pérez Sancho
2nd
Ana Isabel Vela Alonso
4th
María Ugarte Ruiz
5th
Lucas Domínguez Rodríguez
6th
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1177/1040638718805537


CITA ESTA PUBLICACIÓN:

Perez-Sancho M., Vela AI., Horcajo P., Ugarte-Ruiz M., Dominguez L., Fernandez-Garayzabal JF. y de la Fuente R. Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles. Journal of Veterinary Diagnostic Investigation . 30(6):813-820. 2018. (A). ISSN: 1040-6387. DOI: 10.1177/1040638718805537


SERVICIOS: