Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of hemolytic-activity by Listeria strains
Acta Microbiologica Hungarica publica este artículo de investigación
1 de enero de 1990
The validity of CAMP tests with Staphylococcus aureus and Rhodococcus (Corynebacterium) equi as defined for Listeria identification was revised. This characterization method appeared to be unreliable for two reasons: first, a positive CAMP test with R. equi is not specific for Listeria ivanovii as Listeria monocytogenes (and Listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with S. aureus when assaying haemolytic and non-haemolytic Listeria strains (possibility of false negative and false positive results; subjectivity of the interpretation). The use of a Microplate technique previously described instead of CAMP tests is proposed for the reliable demonstration of the haemolytic character of Listeria in the routine identification of these organisms.
Vazquez-Boland JA., Dominguez L., Fernandez-Garayzabal JF., Rodriguez-Ferri E., Briones V., Blanco MM. y Suarez G.
 | Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
