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Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing

Investigación publicada en Veterinary Microbiology

4 de junio de 2014

Diagnostic tests based on cell-mediated immunity are used in programs for eradication of bovine tuberculosis (Mycobacterium bovis). Serological assays could be applied as ancillary methods to detect infected animals. Our objective was to evaluate two serological techniques: M. bovis Ab Test (IDEXX, USA) and Enferplex™ TB assay (Enfer, Ireland) in animals tested simultaneously with the single and comparative intradermal tests and the interferon-gamma assay. This work was performed at two stages. First, a preliminary panel of samples collected prior to intradermal tests from tuberculosis-free (n = 60) and M. bovis-infected herds (n = 78) was assayed, obtaining high specificity: 100% (M. bovis Ab Test) and 98.3% (Enferplex TB assay) but low sensitivity (detection of M. bovis infected animals): 23.9% (M. bovis Ab Test) and 32.6% (Enferplex TB assay). Subsequently, the use of serological techniques was further studied in two herds with M. bovis infection (n = 77) using samples collected prior to, and 72 hours and 15 days after PPD inoculation. The highest level of detection of infected animals for serology was achieved at 15 days post-intradermal tests taking advantage of the anamnestic effect: 70.4% and 85.2% in herd A, and 66.7% and 83.3% in herd B, using M. bovis Ab Test and Enferplex TB assay, respectively. Quantitative results (average values obtained with M. bovis Ab Test ELISA and degree of positivity obtained with Enferplex TB assay) were higher in animals showing lesions compatible with tuberculosis. No significant differences were observed in the number of confirmed infected animals detected with either serological technique.




Casal C., Diez-Guerrier A., Alvarez J., Rodriguez-Campos S., Mateos A., Linscott R., Martel E., Lawrence JC., Whelan A., Clarke J., OBrien A., Dominguez L. y Aranaz A.




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Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing

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Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing


Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

IDEXX Livestock and Poultry Diagnostics.

Enfer Group.

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.







Veterinary Microbiology
FACTOR YEAR Q
2.511 2014

NLMID: 7705469

PMID: 24679958

ISSN: 0378-1135



TÍTULO: Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing


REVISTA: Vet. Microbiol.


NUMERACIÓN: 170(3-4):342-51


AÑO: 2014


EDITORIAL: Elsevier Science Publishers


AUTORES: Casal C., Diez-Guerrier A., Alvarez J., Rodriguez-Campos S., Mateos A., Linscott R., Martel E., Lawrence JC., Whelan A., Clarke J., OBrien A., Dominguez L. and Aranaz A.


PARTICIPANTES VISAVET


2nd
Alberto Antoine Díez Guerrier
3rd
Julio Álvarez Sánchez
12th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.vetmic.2014.02.036


CITA ESTA PUBLICACIÓN:

Casal C., Diez-Guerrier A., Alvarez J., Rodriguez-Campos S., Mateos A., Linscott R., Martel E., Lawrence JC., Whelan A., Clarke J., OBrien A., Dominguez L. y Aranaz A. Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing. Veterinary Microbiology. 170(3-4):342-51. 2014. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2014.02.036


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