Utility of a Panviral Microarray for Detection of Swine Respiratory Viruses in Clinical Samples
Journal of Clinical Microbiology publica este artículo de investigación
1 de abril de 2011
Several factors have recently converged elevating the need for highly parallel diagnostic platforms that have the ability to detect many known, novel, and emerging pathogenic agents simultaneously. Panviral DNA microarrays represent the most robust approach for massively parallel viral surveillance and detection. The Virochip is a panviral DNA microarray that is capable of detecting all known viruses, as well as novel viruses related to known viral families in a single assay, and has been used to successfully identify known and novel viral agents in clinical human specimens. However, the usefulness and the sensitivity of the Virochip platform has not been tested on a set of clinical veterinary specimens with the high degree of genetic variance that is frequently observed in swine virus field isolates. In this report, we investigated the utility and sensitivity of the Virochip to positively detect swine viruses in both cell culture derived samples and clinical swine samples. The Virochip successfully detected PRRSV in serum containing 6.10 X 10(2) viral copies per microliter, and influenza A in lung lavage fluid containing 2.08 X 10(6) viral copies per microliter. The Virochip also successfully detected PCV2 in serum containing 2.50 X 10(8) viral copies per microliter and PRCV in turbinate tissue homogenate. Collectively, the data in this report demonstrate that the Virochip can successfully detect pathogenic viruses frequently found in swine in a variety of solid and liquid specimens, such as turbinate tissue homogenate and lung lavage, as well as antemortem samples, such as serum
Nicholson TL., Kukielka D., Vincent AL., Brockmeier SL., Miller LC. y Faaberg KS.
 | Servicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
 | National Animal Disease Center, Virus and Prion Research Unit. Agricultural Research Service (ARS). U.S. Department of Agriculture (USDA). |
| Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
