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Validation of a new serological assay for the identification of Mycobacterium tuberculosis complex-specific antibodies in pigs and wild boar

Investigación publicada en Preventive Veterinary Medicine

1 de enero de 2019

Background and objectives
Animal tuberculosis (TB) is a multi-host disease involving a wide variety of domestic and wild mammals and causing a significant economic burden and sanitary problems. Wild boar and domestic pigs (Sus scrofa) are indicators of the circulation of the Mycobacterium tuberculosis complex (MTC) and can play a role in its maintenance. The proper diagnosis of MTC contact in these species is, therefore, a key factor as regards controlling TB. The objective of the current study is to evaluate the diagnostic performance of the protein complex P22 as a candidate for use in an in-house ELISA to identify M. tuberculosis complex-specific antibodies for the diagnosis of TB in comparison to the commonly used bPPD-based ELISA (bPPD ELISA) in suids.
Methods
We conducted a retrospective study. Sera were collected from wild boar during hunting season and from domestic pigs during routine handling, and all the animals underwent reference standard tests (detailed necropsy followed by bacteriological culture and isolation). Animal TB was confirmed to be positive in 277 animals and negative in 366 animals based on both reference standard tests. Sera from those animals were tested by P22 ELISA as well as bPPD ELISA.
Results
Both ELISAs yielded a good diagnostic value, however, a higher sensitivity (Se) and specificity (Sp) was achieved with the P22 ELISA (Se: 84.1%; CI95%: 79.3–88.2% / Sp: 98.4%; CI95%:96.5–99.4%) when compared to the bPPD ELISA (Se: 77.3%; CI95%: 71.9–82.2% / Sp: 97.3%; CI95%: 95–98.3%). An optimum Sp of 100% (CI95%: 98.54–100%) was attained with white pigs for both the bPPD and the P22 ELISA.
Discussion
The results suggest that serological tests for MTC-antibody detection, and particularly the P22 ELISA, are valuable tools in the diagnosis of TB in wild boar and domestic pigs when attempting to detect contact with MTC and thereby facilitate TB control and management




Thomas J., Infantes-Lorenzo JA., Moreno I., Cano-Terriza D., de Juan L., Garcia-Bocanegra I., Dominguez L., Dominguez M., Gortazar C. y Risalde MA.




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Validation of a new serological assay for the identification of Mycobacterium tuberculosis complex-specific antibodies in pigs and wild boar



Participantes:

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Indian Council of Agricultural Research (ICAR).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad de CórdobaDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad de Córdoba (UCO).

Hospital Universitario Reina Sofía de Córdoba. Instituto Maimonides de Investigación Biomédica de Cordoba.







Preventive Veterinary Medicine
FACTOR YEAR Q
1.987 2016

NLMID: 8217463

ISSN: 0167-5877



TÍTULO: Validation of a new serological assay for the identification of Mycobacterium tuberculosis complex-specific antibodies in pigs and wild boar


REVISTA: Prev. Vet. Med.


NUMERACIÓN: 162(2019):11-17


AÑO: 2019


EDITORIAL: Elsevier Science Publishers


AUTORES: Thomas J., Infantes-Lorenzo JA., Moreno I., Cano-Terriza D., de Juan L., Garcia-Bocanegra I., Dominguez L., Dominguez M., Gortazar C. and Risalde MA.


PARTICIPANTES VISAVET


2nd
Jose Antonio Infantes Lorenzo
5th
Lucía de Juan Ferré
7th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.prevetmed.2018.11.004


CITA ESTA PUBLICACIÓN:

Thomas J., Infantes-Lorenzo JA., Moreno I., Cano-Terriza D., de Juan L., Garcia-Bocanegra I., Dominguez L., Dominguez M., Gortazar C. y Risalde MA. Validation of a new serological assay for the identification of Mycobacterium tuberculosis complex-specific antibodies in pigs and wild boar. Preventive Veterinary Medicine. 162(2019):11-17. 2019. (A). ISSN: 0167-5877. DOI: 10.1016/j.prevetmed.2018.11.004


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