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Bovine Tuberculosis Protocols Database


Bovine Tuberculosis Protocols Database


Standard Operating Procedures


This section includes the Standard Operating Procedures (SOP) in relation to the Commission Delegated Regulations (EU) 2020/688 and 2020/689 supplementing the Regulation (EU) 2016/429 ("Animal Health Law").


According to article 6 Diagnostic methods (Commission Delegated Regulation (EU) 2020/689), the diagnosis cascade should be followed if a protocol is not available on the EURL website.



Bovine Animals
 
SOP/001/EURL rev1 Intradermal Tuberculin Skin Test Protocol in bovine animals
SOP/004/EURL rev1 Diagnosis of tuberculosis infection in bovine and caprine animals for using the in vitro gamma-interferon detection assay. Applied Biosystems™ Bovigam™ Tb Kit (Thermo Fisher Scientific).

Access to NRLs: Validation report

SOP/006/EURL rev1 Diagnosis of tuberculosis infection in bovine animals for using the in vitro gamma-interferon detection assay. ID Screen® Ruminant IFN-g (IDvet)

Access to NRLs: Validation report


Camelid Animals
 
SOP/003/EURL rev1 Intradermal Tuberculin Skin Test Protocol in camelids
SOP/005/EURL rev1 Detection of antibodies against the Mycobacterium tuberculosis complex for the diagnosis of tuberculosis infection in camelids. Ingezim Tuberculosis DR (Eurofins Technologies).

Access to NRLs: Validation report


Caprine Animals
 
SOP/002/EURL rev1 Intradermal Tuberculin Skin Test Protocol in caprine animals
SOP/004/EURL rev1 Diagnosis of tuberculosis infection in bovine and caprine animals for using the in vitro gamma-interferon detection assay. Applied Biosystems™ Bovigam™ Tb Kit (Thermo Fisher Scientific).

Access to NRLs: Validation report



Guidelines


This section also includes Guidelines for other laboratory methods:


Recovery of Mycobacteria from clinical samples using culture
DNA isolation from tissue samples
Real Time PCR for the detection of Mycobacterium bovis
Real Time PCR for the detection of members of the Mycobacterium tuberculosis complex
Spoligotyping protocol