Standard Operating Procedures

This section includes the Standard Operating Procedures (SOP) in relation to the Commission Delegated Regulations (EU) 2020/688 and 2020/689 supplementing the Regulation (EU) 2016/429 ("Animal Health Law").

According to article 6 Diagnostic methods (Commission Delegated Regulation (EU) 2020/689), the diagnosis cascade should be followed if a protocol is not available on the EURL website.

Bovine Animals
	Intradermal Tuberculin Skin Test Protocol in bovine animals	SOP/001/EURL rev1
	Diagnosis of tuberculosis infection in bovine and caprine animals for using the in vitro gamma-interferon detection assay. Applied Biosystems™ Bovigam™ Tb Kit (Thermo Fisher Scientific). Access to NRLs: Validation report	SOP/004/EURL rev1
	Diagnosis of tuberculosis infection in bovine animals for using the in vitro gamma-interferon detection assay. ID Screen® Ruminant IFN-g (IDvet) Access to NRLs: Validation report	SOP/006/EURL rev1
Camelid Animals
	Intradermal Tuberculin Skin Test Protocol in camelids	SOP/003/EURL rev2
	Detection of antibodies against the Mycobacterium tuberculosis complex for the diagnosis of tuberculosis infection in camelids. Ingezim Tuberculosis DR (Eurofins Technologies). Access to NRLs: Validation report	SOP/005/EURL rev1
Caprine Animals
	Intradermal Tuberculin Skin Test Protocol in caprine animals	SOP/002/EURL rev1
	Diagnosis of tuberculosis infection in bovine and caprine animals for using the in vitro gamma-interferon detection assay. Applied Biosystems™ Bovigam™ Tb Kit (Thermo Fisher Scientific). Access to NRLs: Validation report	SOP/004/EURL rev1

Guidelines

This section also includes Guidelines for other laboratory methods:

	Isolation of Mycobacterium tuberculosis complex microorganisms by culture	SOP/010/EURL
	Extraction of mycobacterial DNA	SOP/008/EURL rev0
	Real Time PCR for the detection of Mycobacterium bovis
	Real time PCR for the detection of MTBC (IS6110)	SOP/007/EURL rev0
	Spoligotyping protocol