Publications Database
Publications Database is an online system designed to list the new scientific publications related to diagnostic techniques (culture, extraction & identification, molecular characterisation and interferon gamma detection).
Periodic searches will be carried out in scientific databases and an e-mail will be sent automatically to all NRLs/EC with the latest updates. A link to the publications will be available.
Last Publications
| Culture | |
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Ferrari S, Zanoni M, Mangeli A, Pigoli C, D´Incau M, Alborali GL, Pacciarini ML, Boniotti MB. Bacteriological culture and direct PCR for detecting the Mycobacterium tuberculosis complex in the Italian eradication campaign: a decade of experience at the National Reference Laboratory. J Appl Microbiol. 135(3):lxae064. 2024. |
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Dwyer R, Witte C, Buss P, Warren R, Miller M, Goosen W. Antemortem detection of Mycobacterium bovis in nasal swabs from African rhinoceros. Sci Rep. 14(1):357. 2024. |
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Didkowska A, Orłowska B, Krajewska-Wędzina M, Augustynowicz-Kopeć E, Brzezińska S, Żygowska M, Wiśniewski J, Kaczor S, Welz M, Olech W, Anusz K. Microbiological and molecular monitoring for bovine tuberculosis in the Polish population of European bison (Bison bonasus). Ann Agric Environ Med. 28(4):575-578. 2021. |
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Ejo M, Haile B, Tariku T, Nigatu S, Kebede E, Bitew AB, Demessie Y, Getaneh G, Alebie A, Girma M, Ota F, Nuru A. Bacteriological and molecular characterization of Mycobacterium bovis isolates from tuberculous lesions collected among slaughtered cattle, Northwest Ethiopia. BMC Microbiol. 21(1):286. 2021. |
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Hlokwe TM, Mogano RM. Utility of xpert® MTB/RIF ultra assay in the rapid diagnosis of bovine tuberculosis in wildlife and livestock animals from South Africa. Prev Vet Med. 2020 Apr;177:104980. 2020. |
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McKinley TJ, Lipschutz-Powell D, Mitchell AP, Wood JLN, Conlan AJK. Risk factors and variations in detection of new bovine tuberculosis breakdowns via slaughterhouse surveillance in Great Britain. PLoS One. 13(6):e0198760. 2018. |
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Hansen RK, Nielsen LH, El Tholth M, Haesler B, Foddai A, Alban L. Comparison of Alternative Meat Inspection Regimes for Pigs From Non-Controlled Housing - Considering the Cost of Error. Front Vet Sci. 5:92. 2018. |
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Ghielmetti G, Friedel U, Scherrer S, Sarno E, Landolt P, Dietz O, Hilbe M, Zweifel C, Stephan R. Non-tuberculous Mycobacteria isolated from lymph nodes and faecal samples of healthy slaughtered cattle and the abattoir environment. Transbound Emerg Dis. 65(3):711-718. 2018. |
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Willeberg PW, McAloon CG, Houtsma E, Higgins I, Clegg TA, More SJ.The Herd-Level Sensitivity of Abattoir Surveillance for Bovine Tuberculosis: Simulating the Effects of Current and Potentially Modified Meat Inspection Procedures in Irish Cattle. Front Vet Sci. 5:82. 2018. |
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Azadi D, Motallebirad T, Ghaffari K, Shojaei H. Mycobacteriosis and Tuberculosis: Laboratory Diagnosis. Open Microbiol J. 12:41-58. 2018. |
| Extraction & identification | |
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Zhu Y, Feng Z, Xu Y, Luo S, Zhang R, Shi X, Wu X, Zhang H. Rapid detection of Mycobacterium tuberculosis based on cyp141 via real-time fluorescence loop-mediated isothermal amplification (cyp141-RealAmp). Front Cell Infect Microbiol. 14:1349063. 2024. |
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Liu Q, Yang M, Zhang H, Ma W, Fu X, Li H, Gao S. A colorimetric tandem combination of CRISPR/Cas12a with dual functional hybridization chain reaction for ultra-sensitive detection of Mycobacterium bovis. Anal Methods. 16(20):3220-3230. 2024. |
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Encinas M, Ferrara Muñiz X, Sammarruco RA, Ruiz Menna V, Garro CJ, Delgado F, Macías A, Magnano G, Zumárraga MJ, Garbaccio SG, Eirin ME. Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis. Front Vet Sci. 11:1359205. 2024. |
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Mabe L, Muthevhuli M, Thekisoe O, Suleman E. Accuracy of molecular diagnostic assays for detection of Mycobacterium bovis: A systematic review and meta-analysis. Prev Vet Med. 226:106190. 2024. |
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Sánchez-Carvajal JM, Vera-Salmoral E, Huerta B, Galán-Relaño Á, Ruedas-Torres I, Larenas-Muñoz F, Luque I, Carrasco L, Gómez-Laguna J. Droplet digital PCR as alternative to microbiological culture for Mycobacterium tuberculosis complex detection in bovine lymph node tissue samples. Front Cell Infect Microbiol. 14:1349999. 2024. |
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Cooke DM, Clarke C, Kerr TJ, Warren RM, Witte C, Miller MA, Goosen WJ. Detection of Mycobacterium bovis in nasal swabs from communal goats (Capra hircus) in rural KwaZulu-Natal, South Africa. Front Microbiol. 15:1349163. 2024. |
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Gomez-Buendia A, Alvarez J, Bezos J, Mourelo J, Amado J, Saez JL, de Juan L, Romero B. Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds. Front Vet Sci. 11:1361788. 2024. |
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Patnaik N, Dey RJ. Label-Free Citrate-Stabilized Silver Nanoparticles-Based, Highly Sensitive, Cost-Effective, and Rapid Visual Method for the Differential Detection of Mycobacterium tuberculosis and Mycobacterium bovis. ACS Infect Dis. Epub ahead of print. 2023. |
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Vera-Salmoral E, Gómez-Laguna J, Galán-Relaño Á, Ruedas-Torres I, Carrasco L, Luque I, Huerta B, Sánchez-Carvajal JM. Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex. Microbiol Spectr. 11(5):e0034823. 2023. |
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Rodríguez-Hernández P, Cardador MJ, Ríos-Reina R, Sánchez-Carvajal JM, Galán-Relaño Á, Jurado-Martos F, Luque I, Arce L, Gómez-Laguna J, Rodríguez-Estévez V. Detection of Mycobacterium tuberculosis complex field infections in cattle using fecal volatile organic compound analysis through gas chromatography-ion mobility spectrometry combined with chemometrics. Microbiol Spectr. 11(5):e0174323. 2023. |
| Immunology | |
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Villafañe L, Rocha RV, Bigi MM, Klepp LI, Taboga OA, Forrellad MA, López MG, Bigi F. Expression and field evaluation of new Mycobacterium bovis antigens. Vet Immunol Immunopathol. 273:110788. 2024. |
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Singhla T, Boonyayatra S, Sthitmatee N, Sirimalaisuwan A, Maicharoen N, Meemey A, Muenthaisong A, Rittipornlertrak A, Sreevatsan S. An ELISA test using a circulating Mycobacterium bovis peptide for detecting bovine tuberculosis in dairy cattle. Front Vet Sci. 11:1384537. 2024. |
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Zhao Y, Fei W, Yang L, Xiang Z, Chen X, Chen Y, Hu C, Chen J, Guo A. The Establishment of a Novel γ-Interferon In Vitro Release Assay for the Differentiation of Mycobacterial Bovis-Infected and BCG-Vaccinated Cattle. Vet Sci. 11(5):198. 2024. |
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O´Brien A, Hayton A, Cutler K, Adler A, Shaw DJ, Clarke J, Watt N, Harkiss GD. Diagnostic accuracy of the Enferplex Bovine TB antibody test using individual milk samples from cattle. PLoS One. 19(4):e0301609. 2024. |
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Jiang D, Song X, Yang L, Zheng L, Niu K, Niu H. Screening of mRNA markers in early bovine tuberculosis blood samples. Front Vet Sci. 11:1330693. 2024. |
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Echeverría G, Zumárraga MJ, Proaño-Pérez F, Blasco FB, de Waard JH. Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis. Sci Rep. 14(1):5155. 2024. |
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Kumar M, Kumar T, Jangir BL, Singh M, Arora D, Bangar Y, Conlan A, Vordermeier M, Bakker D, Byregowda SM, Srinivasan S, Kapur V, Jindal N. Comparative analysis of tuberculin and defined antigen skin tests for detection of bovine tuberculosis in buffaloes (Bubalus bubalis) in Haryana state, India. BMC Vet Res. 20(1):65. 2024. |
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Amato B, Ippolito D, Vitale M, Alduina R, Galluzzo P, Gerace E, Pruiti Ciarello F, Fiasconaro M, Cannella V, Di Marco Lo Presti V. Comparative Study of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis In Vitro Infection in Bovine Bone Marrow Derived Macrophages: Preliminary Results. Microorganisms. 12(2):407. 2024. |
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Batista CM, Veschi JL, de Souza VF, Foti L, Andri LC. Design and development of multiepitope chimeric antigens by bioinformatic and bacterial based recombinant expression methods, with potential application for bovine tuberculosis serodiagnosis. Vet Immunol Immunopathol. 269:110729. 2024. |
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Holder T, Srinivasan S, McGoldrick A, Williams GA, Palmer S, Clarke J, O´Brien A, Conlan AJK, Juleff N, Vordermeier HM, Jones GJ, Kapur V. Temporal dynamics of the early immune response following Mycobacterium bovis infection of cattle. Sci Rep. 14(1):2600. 2024. |
| Molecular characterisation | |
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Pereira AC, Lourenço J, Themudo G, Botelho A, Cunha MV. Population structure and history of Mycobacterium bovis European 3 clonal complex reveal transmission across ecological corridors of unrecognized importance in Portugal. Microbiol Spectr. 12(7):e0382923. 2024. |
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Pereira AC, Pinto D, Cunha MV. First time whole genome sequencing of Mycobacterium bovis from the environment supports transmission at the animal-environment interface. J Hazard Mater. 472:134473. 2024. |
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Refaya AK, Vetrivel U, Palaniyandi K. Genomic Characterization of IS6110 Insertions in Mycobacterium orygis. Evol Bioinform Online. 20:11769343241240558. 2024. |
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Goosen WJ, Moodley S, Ghielmetti G, Moosa Y, Zulu T, Smit T; Vukuzazi Team; Kleynhans L, Kerr TJ, Streicher EM, Hanekom WA, Warren RM, Wong EB, Miller MA. Identification and molecular characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa. One Health. 18:100702. 2024. |
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Maqsood R, Duffy SC, Bin Rashid H, Gill SS, Jabeen C, Arshad N, Umbreen G, Behr MA, Kapur V, Chaudhry M. Molecular detection and characterization of the Mycobacterium tuberculosis complex subspecies responsible for bovine tuberculosis in Punjab, Pakistan. Microbiol Spectr. 12(2):e0269223. 2024. |
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Allen A, Magee R, Devaney R, Ardis T, McNally C, McCormick C, Presho E, Doyle M, Ranasinghe P, Johnston P, Kirke R, Harwood R, Farrell D, Kenny K, Smith J, Gordon S, Ford T, Thompson S, Wright L, Jones K, Prodohl P, Skuce R. Whole-Genome sequencing in routine Mycobacterium bovis epidemiology - scoping the potential. Microb Genom. 10(2):001185. 2024. |
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Pereira AC, Reis AC, Cunha MV. Genomic epidemiology sheds light on the emergence and spread of Mycobacterium bovis Eu2 Clonal Complex in Portugal. Emerg Microbes Infect. 12(2):2253340. 2023. |
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Ghielmetti G, Loubser J, Kerr TJ, Stuber T, Thacker T, Martin LC, O´Hare MA, Mhlophe SK, Okunola A, Loxton AG, Warren RM, Moseley MH, Miller MA, Goosen WJ. Advancing animal tuberculosis surveillance using culture-independent long-read whole-genome sequencing. Front Microbiol. 14:1307440. 2023. |
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Zahran M, El-Shabasy RM, Elrashedy A, Mousa W, Nayel M, Salama A, Zaghawa A, Elsify A. Recent progress in the genotyping of bovine tuberculosis and its rapid diagnosis via nanoparticle-based electrochemical biosensors. RSC Adv. 13(45):31795-31810. 2023. |
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Bakuła Z, Dziurzyński M, Decewicz P, Bakonytė D, Vasiliauskaitė L, Nakčerienė B, Krenke R, Stakėnas P, Jagielski T. Spoligotyping of Mycobacterium tuberculosis - Comparing in vitro and in silico approaches. Infect Genet Evol. 115:105508. 2023. |
| Others | |
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Lakew M, Tadesse B, Srinivasan S, Aschalew M, Andarge B, Kebede D, Etifu A, Alemu T, Yalew B, Benti T, Olani A, Abera S, Bedada W, Fromsa A, Mekonnen GA, Almaw G, Ameni G, Ashenafi H, Gumi B, Bakker D, Kapur V. Assessing the feasibility of test-and-cull and test-and-segregation approaches for the control of high-prevalence bovine tuberculosis in Ethiopian intensive dairy farms. Sci Rep. 14(1):14298. 2024. |
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Tratalos J, Madden J, McGrath G, More S. Computing metrics to inform selection of candidate areas for a regionalized approach to bovine tuberculosis eradication in Ireland. Vet Ital. 60(4). 2024. |
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Gcebe N, Hlokwe TM, Bouw A, Michel A, Rutten VPMG. The Presence of esat-6 and cfp10 and Other Gene Orthologs of the RD 1 Region in Non-Tuberculous Mycobacteria, Mycolicibacteria, Mycobacteroides and Mycolicibacter as Possible Impediments for the Diagnosis of (Animal) Tuberculosis. Microorganisms. 12(6):1151. 2024. |
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Kenny U, Finn S, Barrett D. Private veterinarians´ views of the Irish bovine TB eradication programme. Res Vet Sci. 173:105246. 2024. |
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Badia-Bringué G, Canive M, Vázquez P, Garrido JM, Fernández A, Juste RA, Jiménez JA, González-Recio O, Alonso-Hearn M. Genome-Wide Association Study Reveals Quantitative Trait Loci and Candidate Genes Associated with High Interferon-gamma Production in Holstein Cattle Naturally Infected with Mycobacterium Bovis. Int J Mol Sci. 25(11):6165. 2024. |
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Brunton L, Enticott G. Is badger culling associated with risk compensation behaviour among cattle farmers? Vet Rec. 194(11):e4152. 2024. |
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Barker EN, O´Halloran C, Gunn-Moore DA. Review canine tuberculosis - An emerging concern. Vet J. 305:106111. 2024. |
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Conteddu K, English HM, Byrne AW, Amin B, Griffin LL, Kaur P, Morera-Pujol V, Murphy KJ, Salter-Townshend M, Smith AF, Ciuti S. A scoping review on bovine tuberculosis highlights the need for novel data streams and analytical approaches to curb zoonotic diseases. Vet Res. 55(1):64. 2024. |
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Hernández FA, Tejeda C, Aristizábal B, Raffo E, Moroni M, Salgado M. Confirmation of the invasive American mink (Neogale vison) as carrier of Mycobacterium bovis in southern Chile. Acta Trop. 256:107257. 2024. |
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Watt NJ, Cutler K. ´It is impossible to meet our officially bTB-free targets with the current testing policy´. Vet Rec. 194(9):359. 2024. |
