Publications Database
Publications Database is an online system designed to list the new scientific publications related to diagnostic techniques (culture, extraction & identification, molecular characterisation and interferon gamma detection).
Periodic searches will be carried out in scientific databases and an e-mail will be sent automatically to all NRLs/EC with the latest updates. A link to the publications will be available.
Last Publications
| Culture | |
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Gumbo R, Lourens T, Cooke DM, Kerr TJ, Warren RM, Miller MA, Ghielmetti G, Goosen WJ. Isolation of viable Mycobacterium bovis from faeces of naturally infected free-ranging rural domestic cattle (Bos taurus). J Appl Microbiol. 2025 Nov 28;136(12):lxaf281. |
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Gomez-Buendia A, Triguero-Ocaña R, Pozo P, Muñoz-Mendoza M, Alvarez-Fidalgo M, Mourelo J, Romero B, Alvarez J. Abattoir surveillance: Identifying risk factors associated with bovine tuberculosis lesion detection in a low prevalence region. Prev Vet Med. 2026 Jan;246:106710. |
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de Paula Castro JF, de Souza GO, Taniwaki SA, Heinemann MB, Ferreira Neto JS. Comparison of qPCR methods for the diagnosis of bovine tuberculosis from granulomas collected at slaughterhouses. Braz J Microbiol. 2025 Sep 25. |
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Didkowska A, Klich D, Matusik K, Wilczek M, Anusz K, Krajewska-Wędzina M. Haptoglobin response in serum and pleural fluid of tuberculin reactor cattle assessed by culture and gross pathology. Vet J. 2025 Sep 25;314:106453. |
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Vicenzi JM, Cerva C, Karam FSC, de Moraes LB, Rodrigues RO, Oliveira PL, Bertagnolli AC, Mayer FQ. Accuracy of inspection surveillance in detecting bovine tuberculosis during slaughter in Rio Grande do Sul. Vet Res Commun. 2025 Jun 6;49(4):215. |
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Bovine tuberculosis in cattle slaughtered at Addis Ababa abattoir in Ethiopia and workforce awareness of zoonotic risk. PLoS One. 2025 May 28;20(5):e0321844. |
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Cerva C, de Lima FM, Varela APM, Breyer GM, Vicenzi JM, Bertagnolli AC, Klain VF, Siqueira FM, Mayer FQ. Gut bacterial diversity in bovines infected with Mycobacterium tuberculosis var. bovis: insights on tuberculosis pathogenesis. Tuberculosis (Edinb). 2025 Jul;153:102652. |
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Prajapati SK, Narang D, Chandra M, Sharma A, Singh ST. Detection of tuberculous and non-tuberculous mycobacterial species in trans-tracheal washes of cattle and buffaloes with respiratory distress. Trop Anim Health Prod. 2025 Mar 31;57(3):145. |
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Jiménez-Martín D, Muñoz-Fernández L, Agulló-Ros I, Jiménez-Pizarro N, Cano-Terriza D, García-Bocanegra I, Romero B, Caballero-Gómez J, Gonzálvez M, Rejón S, Martínez R, Risalde MA. Evaluation of techniques for post-mortem diagnosis of Mycobacterium tuberculosis complex infection in goats.Vet Microbiol. 2025 Mar 19;304:110485. |
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Mignacca SA, Sánchez-Miguel C, Salgado S, Brady C, Fagan S, Murphy D, Sheahan M, Kennedy A, Casey M, Johnson A, McGettrick S, McElroy M, Moriarty J. Pathology of new world camelids in Ireland: 13 years of disease surveillance and the significance of bovine tuberculosis in alpaca. Prev Vet Med. 2025 Mar 12;239:106510. |
| Extraction & identification | |
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Zhu Y, Feng Z, Xu Y, Luo S, Zhang R, Shi X, Wu X, Zhang H. Rapid detection of Mycobacterium tuberculosis based on cyp141 via real-time fluorescence loop-mediated isothermal amplification (cyp141-RealAmp). Front Cell Infect Microbiol. 14:1349063. 2024. |
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Liu Q, Yang M, Zhang H, Ma W, Fu X, Li H, Gao S. A colorimetric tandem combination of CRISPR/Cas12a with dual functional hybridization chain reaction for ultra-sensitive detection of Mycobacterium bovis. Anal Methods. 16(20):3220-3230. 2024. |
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Encinas M, Ferrara Muñiz X, Sammarruco RA, Ruiz Menna V, Garro CJ, Delgado F, Macías A, Magnano G, Zumárraga MJ, Garbaccio SG, Eirin ME. Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis. Front Vet Sci. 11:1359205. 2024. |
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Mabe L, Muthevhuli M, Thekisoe O, Suleman E. Accuracy of molecular diagnostic assays for detection of Mycobacterium bovis: A systematic review and meta-analysis. Prev Vet Med. 226:106190. 2024. |
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Sánchez-Carvajal JM, Vera-Salmoral E, Huerta B, Galán-Relaño Á, Ruedas-Torres I, Larenas-Muñoz F, Luque I, Carrasco L, Gómez-Laguna J. Droplet digital PCR as alternative to microbiological culture for Mycobacterium tuberculosis complex detection in bovine lymph node tissue samples. Front Cell Infect Microbiol. 14:1349999. 2024. |
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Cooke DM, Clarke C, Kerr TJ, Warren RM, Witte C, Miller MA, Goosen WJ. Detection of Mycobacterium bovis in nasal swabs from communal goats (Capra hircus) in rural KwaZulu-Natal, South Africa. Front Microbiol. 15:1349163. 2024. |
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Gomez-Buendia A, Alvarez J, Bezos J, Mourelo J, Amado J, Saez JL, de Juan L, Romero B. Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds. Front Vet Sci. 11:1361788. 2024. |
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Patnaik N, Dey RJ. Label-Free Citrate-Stabilized Silver Nanoparticles-Based, Highly Sensitive, Cost-Effective, and Rapid Visual Method for the Differential Detection of Mycobacterium tuberculosis and Mycobacterium bovis. ACS Infect Dis. Epub ahead of print. 2023. |
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Vera-Salmoral E, Gómez-Laguna J, Galán-Relaño Á, Ruedas-Torres I, Carrasco L, Luque I, Huerta B, Sánchez-Carvajal JM. Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex. Microbiol Spectr. 11(5):e0034823. 2023. |
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Rodríguez-Hernández P, Cardador MJ, Ríos-Reina R, Sánchez-Carvajal JM, Galán-Relaño Á, Jurado-Martos F, Luque I, Arce L, Gómez-Laguna J, Rodríguez-Estévez V. Detection of Mycobacterium tuberculosis complex field infections in cattle using fecal volatile organic compound analysis through gas chromatography-ion mobility spectrometry combined with chemometrics. Microbiol Spectr. 11(5):e0174323. 2023. |
| Immunology | |
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Palphramand KL, Anderson P, Bellamy F, Birch C, Davé D, Eckery D, Gomm M, Jinks R, Massei G, Pinkham R, Rahou S, Simmons H, Vial F, Williams GA, Smith GC. Immune response to co-administration of Bacillus Calmette-Guérin (BCG) and contraceptive vaccines in badgers (Meles meles). Vaccine. 2026 Jan 25;71:128069. |
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Sharma S, Filia G, Leishangthem GD, Sethi RS, Kaur G. Studies on the immunological biomarkers of bovine tuberculosis in naturally infected cattle. Braz J Microbiol. 2025 Dec 9;57(1):1. |
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Camussone C, Moyano RD, Alonso MN, Cicotello J, Peña A, Molineri AI, Astesana DM, Suárez-Archilla G, Tello D´Elia F, Aliprandi D, Welschen NM, Signorini ML, Zbrun MV. Strategic use of enzyme-linked immunosorbent assay as a complementary tool for the diagnosis of bovine tuberculosis. Res Vet Sci. 2025 Dec 4;199:106017. |
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Chileshe J, Chen JM, Shury T. Evaluation of an interferon-gamma release assay for the detection of Mycobacterium bovis using QuantiFERON-TB gold tubes in bison (Bison bison athabascae). Vet Immunol Immunopathol. 2025 Dec;290:111027. |
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Bueno NMM, Reck C, Nogueira LL, Pinto AR, Menin Á. Development and evaluation of an ELISA based on recombinant Mb1454 antigen for the detection of specific anti-Mycobacterium bovis antibodies in cattle. Vet Immunol Immunopathol. 2025 Dec;290:111025. |
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Giovannozzi S, Martucciello A, Rodríguez MD, Iruela IM, Boifava M, Schiavo L, Viscito A, Parisio G, Vitale N, Bezos J, De Carlo E, Boniotti MB. Evaluation of serological assays for intra vitam diagnosis of bovine tuberculosis in water buffalo (Bubalus bubalis). Front Microbiol. 2025 Nov 12;16:1684425. |
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Agulló-Ros I, Muñoz-Fernández L, Roy Á, Bezos J, Sevilla IA, Moreno I, Garrido J, Rodríguez-Bertos A, Domínguez M, Juste R, Domínguez L, Gortázar C, Risalde MA. Protective effect of heat-inactivated Mycobacterium bovis applied intramuscularly is associated with enhanced lung immune response in caprine tuberculosis. Vet Res. 2025 Nov 4;56(1):209. |
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Modeling immune responses of cattle to Mycobacterium bovis using magnetic bioprinted granulomas. mSphere. 2025 Nov 25;10(11):e0059525. |
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Turgenbayev K, Borsynbayeva A, Ozatbekuly A, Dyusenov S, Tlepov A, Turgenbayev R. Development and evaluation of a formaldehyde-stabilized tuberculin as a safe and potent alternative to phenol-based purified protein derivative for the diagnosis of animal tuberculosis. Vet World. 2025 Oct;18(10):3268-3287. |
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Juste RA, Sevilla IA, Minguijon E, Fuertes M, Elguezabal N, Geijo M, Vazquez P, Serrano M, Rakel A, Gortazar C, Etxezarreta A, Molina E, Garrido JM. Tuberculosis vaccination: microbiological and immunological summary of a series of experimental challenge studies. Vet Res. 2025 Oct 24;56(1):202. |
| Molecular characterisation | |
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Verma R, Gandham RK, Mathesh K, Kumar D. First report of whole genome sequence of a Mycobacterium bovis (3/86Rv) strain isolated from a cow in India. Braz J Microbiol. 2025 Dec 28;57(1):27. |
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Wang H, Xia X, Zeng L, Yang J, Han J, MacHugh DE, Lenstra JA, Song Y, Fan A, Zhu Y, Zhu Z, Zhang X, Chen Y, Han J, Lei C, Chen N, Zhang Y, Gao Y. The Indicine X-linked CYBBL237M Mutation Can Suppress Intracellular Infection with Tubercle Bacilli. Genomics Proteomics Bioinformatics. 2025 Dec 23:qzaf131. |
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Fan A, Guo Y, Li Z, Dong X, Zhang Z, Wang X, Song Y, Han J, Yang J, Wang H, Zhang X, Zhang Y, Gao Y. Species-specific alternative splicing of SP110 drives tuberculosis susceptibility in cattle. Vet Res. 2025 Dec 12. |
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O´Shaughnessy J, Harvey N, Byrne B, McElroy M, Gutierrez M, Murphy D, Kenny K, Cameron H, Prendergast D, Cupial R, Goggin M, Dygico LK, Smith J, Tratalos JA, Devaney R, Ranasinghe P, Ardis T, Allen A, McGrath G, Gordon SV, Farrell D. The genomic diversity and spatial patterns of Mycobacterium bovis in Ireland revealed by whole genome sequencing. Ir Vet J. 2025 Dec 3. |
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Harvey N, McGrath G, O´Shaughnessy J, Tratalos JA, Byrne B, Allen A, Skuce R, Kenny K, Gordon SV, Farrell D. Integrating whole-genome sequencing and epidemiology to characterise Mycobacterium bovis transmission in Ireland: a proof of concept. Ir Vet J. 2025 Dec 1;79(1):3. |
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Dane H, Gilbride B, Thomas M, Grant IR. Evidence of mycobacteriaemias and mycobacterial co-infections uncovered in cattle at slaughter using a novel phage-based PhMS-qPCR assay for viable Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis. Ir Vet J. 2025 Nov 29;79(1):2. |
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Gormley E, MacHugh DE, Meade KG. Epigenetic regulation of the immune response to Mycobacterium bovis infection in cattle: potential implications for diagnostic test sensitivity. Tuberculosis (Edinb). 2025 Dec;155:102697. |
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Farrell D, Perets V, Gordon SV. SNiPgenie: a tool for microbial SNP site detection from whole-genome sequencing data. Access Microbiol. 2025 Sep 22;7(9):001021.v3. |
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Elsayed MSA, Alqaim ZH, Fayed AM, Eldsouky SM, Basiouny MS, Metwally AM, Abdelbadee A, Hasan AS, ElAlfy AKEM, Nasr MA, Wahdan SME, Elsayed RA, Anwer MM, El-Bahy AM, Salah A. Genetic diversity and antibiotic resistance of Mycobacterium bovis in bovines in the Delta area of Egypt. Front Cell Infect Microbiol. 2025 Sep 12;15:1600225. |
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Zhang S, Zhao H, Guo Q, Xue R, Jiang Z, Jiang W, Xing L, Wei X, Diao Y, Tang Y, Lan Z, Zhang Y. Establishment and application of a TaqMan-based quantitative PCR assay for simultaneous detection of bovine Brucella spp. and Mycobacterium spp. Front Microbiol. 2025 Sep 12;16:1633809. |
| Others | |
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Villanueva-Cabezas JP, Dorjee S, McKinley J, Pem R, Rinchen S. Dairy farmers have minimal knowledge of bovine tuberculosis: A cross-sectional study in Bhutan. PLoS Negl Trop Dis. 2025 Dec 22;19(12):e0013817. |
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Alonso-Moreno P, Ortiz P, Ortega J, Velasco C, López A, Bezos J, Izquierdo-Garcia JL. Metabolomic signatures of tuberculosis and paratuberculosis in goats revealed by high-resolution and benchtop NMR spectroscopy. Vet Res. 2025 Dec 12. |
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Scott-Baumann J, Beckmann M, Villarreal-Ramos B, Rooke D, Hewinson G, Delahay RJ, Mur LAJ. Metabolomic changes in the peripheral blood of wild trapped badgers (Meles meles) correlate with bovine tuberculosis (Mycobacterium bovis) infection status. BMC Vet Res. 2025 Dec 9. |
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Rinchen S, Dorjee S, Van Der Zwan A, Pem R, Campbell PT, Villanueva-Cabezas JP. Dairy consumers´ knowledge, attitudes, and practices towards zoonotic bovine tuberculosis in a country without veterinary and public health interventions: A cross-sectional study in Bhutan. Prev Vet Med. 2026 Jan;246:106714. |
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Willgert K, Cliff M, Meinke S, Messina D, Broom DM, Wood J, Conlan AJK. Burden of Bovine Tuberculosis on Animal Health, Welfare and Production: A Systematic Review. Transbound Emerg Dis. 2025 Oct 7;2025:6541298. |
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Gebremichael D. Epidemiological Trend Analysis of Bovine Tuberculosis and Its Public Health Impact in Ethiopia. J Trop Med. 2025 Oct 5;2025:2727632. |
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Szacawa E, Radulski Ł, Weiner M, Szulowski K, Krajewska-Wędzina M. Mycobacterium tuberculosis Complex Infections in Animals: A Comprehensive Review of Species Distribution and Laboratory Diagnostic Methods. Pathogens. 2025 Oct 4;14(10):1004. |
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Desu M, Fasil N, Abebe R. Apparent prevalence, lesion distribution and risk factors of bovine tuberculosis in cattle slaughtered at the Shashemene and Arsi Negelle municipal abattoirs, Ethiopia. One Health. 2025 Sep 15;21:101200. |
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Asfaw LS. Perception of cattle owners towards risk of raw milk consumption for bovine tuberculosis transmission in Hosanna, Central Ethiopia: A community-based cross-sectional study. PLoS Negl Trop Dis. 2025 Aug 12;19(8):e0013355. |
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Djimramadji H, Arino J, Djomegni PMT, Haggar MSD. Dynamics of bovine tuberculosis transmission in mixed herds in Chad. Math Biosci. 2025 Sep;387:109503. |
