Publications Database
Publications Database is an online system designed to list the new scientific publications related to diagnostic techniques (culture, extraction & identification, molecular characterisation and interferon gamma detection).
Periodic searches will be carried out in scientific databases and an e-mail will be sent automatically to all NRLs/EC with the latest updates. A link to the publications will be available.
Last Publications
| Culture | |
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de Paula Castro JF, de Souza GO, Taniwaki SA, Heinemann MB, Ferreira Neto JS. Comparison of qPCR methods for the diagnosis of bovine tuberculosis from granulomas collected at slaughterhouses. Braz J Microbiol. 2025 Sep 25. |
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Didkowska A, Klich D, Matusik K, Wilczek M, Anusz K, Krajewska-Wędzina M. Haptoglobin response in serum and pleural fluid of tuberculin reactor cattle assessed by culture and gross pathology. Vet J. 2025 Sep 25;314:106453. |
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Vicenzi JM, Cerva C, Karam FSC, de Moraes LB, Rodrigues RO, Oliveira PL, Bertagnolli AC, Mayer FQ. Accuracy of inspection surveillance in detecting bovine tuberculosis during slaughter in Rio Grande do Sul. Vet Res Commun. 2025 Jun 6;49(4):215. |
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Bovine tuberculosis in cattle slaughtered at Addis Ababa abattoir in Ethiopia and workforce awareness of zoonotic risk. PLoS One. 2025 May 28;20(5):e0321844. |
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Cerva C, de Lima FM, Varela APM, Breyer GM, Vicenzi JM, Bertagnolli AC, Klain VF, Siqueira FM, Mayer FQ. Gut bacterial diversity in bovines infected with Mycobacterium tuberculosis var. bovis: insights on tuberculosis pathogenesis. Tuberculosis (Edinb). 2025 Jul;153:102652. |
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Prajapati SK, Narang D, Chandra M, Sharma A, Singh ST. Detection of tuberculous and non-tuberculous mycobacterial species in trans-tracheal washes of cattle and buffaloes with respiratory distress. Trop Anim Health Prod. 2025 Mar 31;57(3):145. |
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Jiménez-Martín D, Muñoz-Fernández L, Agulló-Ros I, Jiménez-Pizarro N, Cano-Terriza D, García-Bocanegra I, Romero B, Caballero-Gómez J, Gonzálvez M, Rejón S, Martínez R, Risalde MA. Evaluation of techniques for post-mortem diagnosis of Mycobacterium tuberculosis complex infection in goats.Vet Microbiol. 2025 Mar 19;304:110485. |
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Mignacca SA, Sánchez-Miguel C, Salgado S, Brady C, Fagan S, Murphy D, Sheahan M, Kennedy A, Casey M, Johnson A, McGettrick S, McElroy M, Moriarty J. Pathology of new world camelids in Ireland: 13 years of disease surveillance and the significance of bovine tuberculosis in alpaca. Prev Vet Med. 2025 Mar 12;239:106510. |
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Lyu J, Narum DE, Baldwin SL, Larsen SE, Bai X, Griffith DE, Dartois V, Naidoo T, Steyn AJC, Coler RN, Chan ED. Understanding the development of tuberculous granulomas: insights into host protection and pathogenesis, a review in humans and animals. Front Immunol. 2024 Dec 9;15:1427559 |
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Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata. BMC Vet Res. 2024 Oct 18;20(1):478. |
| Extraction & identification | |
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Zhu Y, Feng Z, Xu Y, Luo S, Zhang R, Shi X, Wu X, Zhang H. Rapid detection of Mycobacterium tuberculosis based on cyp141 via real-time fluorescence loop-mediated isothermal amplification (cyp141-RealAmp). Front Cell Infect Microbiol. 14:1349063. 2024. |
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Liu Q, Yang M, Zhang H, Ma W, Fu X, Li H, Gao S. A colorimetric tandem combination of CRISPR/Cas12a with dual functional hybridization chain reaction for ultra-sensitive detection of Mycobacterium bovis. Anal Methods. 16(20):3220-3230. 2024. |
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Encinas M, Ferrara Muñiz X, Sammarruco RA, Ruiz Menna V, Garro CJ, Delgado F, Macías A, Magnano G, Zumárraga MJ, Garbaccio SG, Eirin ME. Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis. Front Vet Sci. 11:1359205. 2024. |
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Mabe L, Muthevhuli M, Thekisoe O, Suleman E. Accuracy of molecular diagnostic assays for detection of Mycobacterium bovis: A systematic review and meta-analysis. Prev Vet Med. 226:106190. 2024. |
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Sánchez-Carvajal JM, Vera-Salmoral E, Huerta B, Galán-Relaño Á, Ruedas-Torres I, Larenas-Muñoz F, Luque I, Carrasco L, Gómez-Laguna J. Droplet digital PCR as alternative to microbiological culture for Mycobacterium tuberculosis complex detection in bovine lymph node tissue samples. Front Cell Infect Microbiol. 14:1349999. 2024. |
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Cooke DM, Clarke C, Kerr TJ, Warren RM, Witte C, Miller MA, Goosen WJ. Detection of Mycobacterium bovis in nasal swabs from communal goats (Capra hircus) in rural KwaZulu-Natal, South Africa. Front Microbiol. 15:1349163. 2024. |
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Gomez-Buendia A, Alvarez J, Bezos J, Mourelo J, Amado J, Saez JL, de Juan L, Romero B. Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds. Front Vet Sci. 11:1361788. 2024. |
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Patnaik N, Dey RJ. Label-Free Citrate-Stabilized Silver Nanoparticles-Based, Highly Sensitive, Cost-Effective, and Rapid Visual Method for the Differential Detection of Mycobacterium tuberculosis and Mycobacterium bovis. ACS Infect Dis. Epub ahead of print. 2023. |
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Vera-Salmoral E, Gómez-Laguna J, Galán-Relaño Á, Ruedas-Torres I, Carrasco L, Luque I, Huerta B, Sánchez-Carvajal JM. Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex. Microbiol Spectr. 11(5):e0034823. 2023. |
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Rodríguez-Hernández P, Cardador MJ, Ríos-Reina R, Sánchez-Carvajal JM, Galán-Relaño Á, Jurado-Martos F, Luque I, Arce L, Gómez-Laguna J, Rodríguez-Estévez V. Detection of Mycobacterium tuberculosis complex field infections in cattle using fecal volatile organic compound analysis through gas chromatography-ion mobility spectrometry combined with chemometrics. Microbiol Spectr. 11(5):e0174323. 2023. |
| Immunology | |
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Blanco FC, Onnainty R, Marini MR, Klepp LI, Rocha RV, Villafañe LA, Vazquez CL, Canal A, Granero G, Bigi F. A DIVA-compatible Mycobacterium bovis triple mutant vaccine confers protection against bovine tuberculosis in mouse model. Vet Immunol Immunopathol. 2025 Sep 17;289:111001. |
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Flores-Villalva S, De Matteis G, Grandoni F, Scatà MC, Donniacuo A, Schiavo L, Franzoni G, Mazzone P, Elnaggar M, De Carlo E, Galiero G, Davis WC, Martucciello A. Polyfunctionality of CD4+ T lymphocytes in buffaloes and cattle: comparative antigen-specific cytokine responses in bovine tuberculosis infection. Front Immunol. 2025 Sep 8;16:1608065. |
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Jeong JK, Lim MN, Kim KJ. Fetal Bovine Serum Supplementation Enhances Functional Consistency of IGRA Results in Bovine Tuberculosis Diagnostics. Animals (Basel). 2025 Sep 2;15(17):2580. |
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Middleton S, Singh M, Coad M, Palmer S, Holder T, Steinbach S, Hardiman R, Vordermeier HM, Jones GJ. Optimization of a molecularly defined tuberculin formulation: recombinant fusion proteins and epitope surgery. J Clin Microbiol. 2025 Oct 8;63(10):e0055225. |
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Menin Á, Bueno NMM, Hindlmayer ME, Reck C, de Lima Nogueira L, Pinto AR, Báfica A. BLV coinfection impairs immunity and diagnostics in bovine tuberculosis. Sci Rep. 2025 Aug 19;15(1):30286. |
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Khalid H, Coad M, Dry I, McGuinnes C, Waddell LA, Hope JC, Wu Z. Development and characterization of monoclonal antibodies specific for bovine IP-10. Vet Res. 2025 Aug 14;56(1):169. |
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Fernández-Veiga L, Fuertes M, Geijo MV, Elguezabal N, Serrano-Mestre JL, Vázquez-Iniesta L, Prados-Rosales R, Michelet L, Boschiroli ML, Pérez de Val B, Jones GJ, Juste RA, Garrido JM, Sevilla IA. Protection and diagnostic interference induced by heat-inactivated, phage-inactivated and live vaccine prototypes against animal tuberculosis. Front Vet Sci. 2025 Jul 21;12:1620497. |
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Smith GC, Budgey R. Bovine tuberculosis model validation against a field study of badger vaccination with selective culling. PLoS One. 2025 Jul 21;20(7):e0320830. |
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Faysal MA, Chowdhury MSR, Tanni FY, Hossain H, Brishty KA, Uddin MB, Rahman MM, Rahman MM, Hossain MM. Sero-Prevalence and Molecular Detection of Mycobacterium bovis in Cattle at Sylhet Division of Bangladesh. Vet Med Sci. 2025 Sep;11(5):e70531. |
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Blanco FC, Onnainty R, Marini MR, Klepp LI, García EA, Vazquez CL, Canal A, Granero G, Bigi F. M72 Fusion Proteins in Nanocapsules Enhance BCG Efficacy Against Bovine Tuberculosis in a Mouse Model. Pathogens. 2025 Jun 16;14(6):592. |
| Molecular characterisation | |
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Farrell D, Perets V, Gordon SV. SNiPgenie: a tool for microbial SNP site detection from whole-genome sequencing data. Access Microbiol. 2025 Sep 22;7(9):001021.v3. |
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Elsayed MSA, Alqaim ZH, Fayed AM, Eldsouky SM, Basiouny MS, Metwally AM, Abdelbadee A, Hasan AS, ElAlfy AKEM, Nasr MA, Wahdan SME, Elsayed RA, Anwer MM, El-Bahy AM, Salah A. Genetic diversity and antibiotic resistance of Mycobacterium bovis in bovines in the Delta area of Egypt. Front Cell Infect Microbiol. 2025 Sep 12;15:1600225. |
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Zhang S, Zhao H, Guo Q, Xue R, Jiang Z, Jiang W, Xing L, Wei X, Diao Y, Tang Y, Lan Z, Zhang Y. Establishment and application of a TaqMan-based quantitative PCR assay for simultaneous detection of bovine Brucella spp. and Mycobacterium spp. Front Microbiol. 2025 Sep 12;16:1633809. |
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Bertasio C, Carta V, Parisio G, Zanoni M, Tamba M, Mazzera L, Scaltriti E, Pacciarini ML, Alborali GL, Polzer D, Nigsch A, Steinparzer R, Boniotti MB. M. caprae in northern Italy: a comprehensive analysis through whole-genome sequencing on the genetic variability in bovine herds. Vet Res. 2025 Aug 7;56(1):163. |
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Zhou H, Wu W, Zhang Q, Zhang T, Jiang S, Liu H, Ma Y, Chang L, Xie Y, Zhu J, Zhou D, Zhang Y, Xu P. Quantitative Proteome Analysis of Plasma Extracellular Vesicles Identifies Three Proteins with Potential Diagnostic Value for Mycobacterium bovis Infection in Cows. J Proteome Res. 2025 Jun 6;24(6):2926-2938. |
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Szacawa E, Kozieł N, Brzezińska S, Augustynowicz-Kopeć E, Weiner M, Szulowski K, Krajewska-Wędzina M. Laboratory Diagnosis of Animal Tuberculosis in Tracing Interspecies Transmission of Mycobacterium bovis. Pathogens. 2025 May 7;14(5):459. |
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Wei Y, Yuan M, Zhang Y, Gao Y. The 2SP Site Mutation in the Bovine Natural Resistance-Associated Macrophage 1 Promoter Exhibits Antituberculosis Potential. Int J Mol Sci. 2025 Apr 29;26(9):4229. |
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Lorente-Leal V, Pozo P, Bezos J, Collado S, Vicente J, Stuber T, Álvarez J, de Juan L, Romero B. Revisiting Mycobacterium bovis SB0121 genetic diversity reflects the complexity behind bovine tuberculosis persistence in Spain. Prev Vet Med. 2025 Jun;239:106519. |
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Sandhu P, Nunez-Garcia J, Berg S, Wheeler J, Dale J, Upton P, Gibbens J, Hewinson RG, Downs SH, Ellis RJ, Palkopoulou E. Enhanced analysis of the genomic diversity of Mycobacterium bovis in Great Britain to aid control of bovine tuberculosis. Front Microbiol. 2025 Mar 25;16:1515906. |
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O´Grady JF, McHugo GP, Ward JA, Hall TJ, Faherty O´Donnell SL, Correia CN, Browne JA, McDonald M, Gormley E, Riggio V, Prendergast JGD, Clark EL, Pausch H, Meade KG, Gormley IC, Gordon SV, MacHugh DE. Integrative genomics sheds light on the immunogenetics of tuberculosis in cattle. Commun Biol. 2025 Mar 24;8(1):479. |
| Others | |
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Desu M, Fasil N, Abebe R. Apparent prevalence, lesion distribution and risk factors of bovine tuberculosis in cattle slaughtered at the Shashemene and Arsi Negelle municipal abattoirs, Ethiopia. One Health. 2025 Sep 15;21:101200. |
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Asfaw LS. Perception of cattle owners towards risk of raw milk consumption for bovine tuberculosis transmission in Hosanna, Central Ethiopia: A community-based cross-sectional study. PLoS Negl Trop Dis. 2025 Aug 12;19(8):e0013355. |
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Djimramadji H, Arino J, Djomegni PMT, Haggar MSD. Dynamics of bovine tuberculosis transmission in mixed herds in Chad. Math Biosci. 2025 Sep;387:109503. |
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Vargas Campos CA, Chevillon C, Sylla A, Dogbe M, Fast KM, Pechal J, Rakestraw A, Scott ME, Sandel MW, Jordan H, Benbow ME, Guégan JF. Seasonality and mycobacterial infectious diseases in animals and humans: is there a generality of seasonal patterns for mycobacterial infections? Infect Dis Poverty. 2025 Jul 3;14(1):59. |
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George BM, Duncan D, Waller E, Marriott E, Payne MC, Withenshaw S, Brouwer A, Harris KA, May HE, Avigad R. Bovine TB infection status in cattle in Great Britain in 2023. Vet Rec. 2025 Jun 21;196(12):e5696. |
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Palmer MV, Jones DE, Bockenstedt NJ, Boggiatto PM. Tertiary lymphoid structures in pulmonary granulomas of cattle experimentally infected with aerosolized Mycobacterium bovis. BMC Vet Res. 2025 Jun 5;21(1):403. |
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Peruzy MF, Houf K, Ambrosio RL, Sorlini GML, Ceccarelli M, Tomei F, Murru N. Evaluating remote post-mortem inspection in bovines: Challenges, limitations, and future potential in assessing post-mortem outcomes in cattle offal. Food Res Int. 2025 Aug;214:116644. |
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Commandeur S, van der Most M, Koomen J, van Keulen L, Dinkla A, Luinenburg X, Escher M, Jacobs P, Keur I, Grinwis GCM, Weerts E, Broens EM, Anthony R, Agterveld MK, Rebel K, Huisman E, Heijne M, Koets A. Mycobacterium bovis infected domestic cats in an officially bovine tuberculosis free country resulting in human infection. One Health. 2025 Apr 21;20:101048. |
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Holmes EL, O´Hagan MJH, Menzies FD, Byrne AW, McBride KR, McCormick CM, Scantlebury DM, Reid N. Landscape as a Shared Space for Badgers and Cattle: Insights Into Indirect Contact and Bovine Tuberculosis Transmission Risk. Ecol Evol. 2025 Apr 15;15(4):e71114. |
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Balks E, Trösemeier JH, Mühlebach MD, Reuter A. Mass spectrometry analysis of bovine tuberculins - 3R potential in batch potency testing. Biologicals. 2025 May;90:101825. |
