Publications Database
Publications Database is an online system designed to list the new scientific publications related to diagnostic techniques (culture, extraction & identification, molecular characterisation and interferon gamma detection).
Periodic searches will be carried out in scientific databases and an e-mail will be sent automatically to all NRLs/EC with the latest updates. A link to the publications will be available.
Last Publications
| Culture | |
|
Vicenzi JM, Cerva C, Karam FSC, de Moraes LB, Rodrigues RO, Oliveira PL, Bertagnolli AC, Mayer FQ. Accuracy of inspection surveillance in detecting bovine tuberculosis during slaughter in Rio Grande do Sul. Vet Res Commun. 2025 Jun 6;49(4):215. |
|
|
Bovine tuberculosis in cattle slaughtered at Addis Ababa abattoir in Ethiopia and workforce awareness of zoonotic risk. PLoS One. 2025 May 28;20(5):e0321844. |
|
|
Cerva C, de Lima FM, Varela APM, Breyer GM, Vicenzi JM, Bertagnolli AC, Klain VF, Siqueira FM, Mayer FQ. Gut bacterial diversity in bovines infected with Mycobacterium tuberculosis var. bovis: insights on tuberculosis pathogenesis. Tuberculosis (Edinb). 2025 Jul;153:102652. |
|
|
Prajapati SK, Narang D, Chandra M, Sharma A, Singh ST. Detection of tuberculous and non-tuberculous mycobacterial species in trans-tracheal washes of cattle and buffaloes with respiratory distress. Trop Anim Health Prod. 2025 Mar 31;57(3):145. |
|
|
Jiménez-Martín D, Muñoz-Fernández L, Agulló-Ros I, Jiménez-Pizarro N, Cano-Terriza D, García-Bocanegra I, Romero B, Caballero-Gómez J, Gonzálvez M, Rejón S, Martínez R, Risalde MA. Evaluation of techniques for post-mortem diagnosis of Mycobacterium tuberculosis complex infection in goats.Vet Microbiol. 2025 Mar 19;304:110485. |
|
|
Mignacca SA, Sánchez-Miguel C, Salgado S, Brady C, Fagan S, Murphy D, Sheahan M, Kennedy A, Casey M, Johnson A, McGettrick S, McElroy M, Moriarty J. Pathology of new world camelids in Ireland: 13 years of disease surveillance and the significance of bovine tuberculosis in alpaca. Prev Vet Med. 2025 Mar 12;239:106510. |
|
|
Lyu J, Narum DE, Baldwin SL, Larsen SE, Bai X, Griffith DE, Dartois V, Naidoo T, Steyn AJC, Coler RN, Chan ED. Understanding the development of tuberculous granulomas: insights into host protection and pathogenesis, a review in humans and animals. Front Immunol. 2024 Dec 9;15:1427559 |
|
|
Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata. BMC Vet Res. 2024 Oct 18;20(1):478. |
|
|
Liebler-Tenorio EM, Wedlich N, Figl J, Köhler H, Ulrich R, Schröder C, Rissmann M, Grode L, Kaufmann SHE, Menge C. Challenge Dose Titration in a Mycobacterium bovis Infection Model in Goats. Int J Mol Sci. 2024 Sep 10;25(18):9799. |
|
|
Palmer MV, Kanipe C, Hwang S, Thacker TC, Lehman KA, Ledesma NA, Gustafson KK, Boggiatto PM. Pathogen Detection in Early Phases of Experimental Bovine Tuberculosis. Vet Sci. 2024 Aug 7;11(8):357. |
| Extraction & identification | |
|
|
Zhu Y, Feng Z, Xu Y, Luo S, Zhang R, Shi X, Wu X, Zhang H. Rapid detection of Mycobacterium tuberculosis based on cyp141 via real-time fluorescence loop-mediated isothermal amplification (cyp141-RealAmp). Front Cell Infect Microbiol. 14:1349063. 2024. |
|
|
Liu Q, Yang M, Zhang H, Ma W, Fu X, Li H, Gao S. A colorimetric tandem combination of CRISPR/Cas12a with dual functional hybridization chain reaction for ultra-sensitive detection of Mycobacterium bovis. Anal Methods. 16(20):3220-3230. 2024. |
|
|
Encinas M, Ferrara Muñiz X, Sammarruco RA, Ruiz Menna V, Garro CJ, Delgado F, Macías A, Magnano G, Zumárraga MJ, Garbaccio SG, Eirin ME. Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis. Front Vet Sci. 11:1359205. 2024. |
|
|
Mabe L, Muthevhuli M, Thekisoe O, Suleman E. Accuracy of molecular diagnostic assays for detection of Mycobacterium bovis: A systematic review and meta-analysis. Prev Vet Med. 226:106190. 2024. |
|
|
Sánchez-Carvajal JM, Vera-Salmoral E, Huerta B, Galán-Relaño Á, Ruedas-Torres I, Larenas-Muñoz F, Luque I, Carrasco L, Gómez-Laguna J. Droplet digital PCR as alternative to microbiological culture for Mycobacterium tuberculosis complex detection in bovine lymph node tissue samples. Front Cell Infect Microbiol. 14:1349999. 2024. |
|
|
Cooke DM, Clarke C, Kerr TJ, Warren RM, Witte C, Miller MA, Goosen WJ. Detection of Mycobacterium bovis in nasal swabs from communal goats (Capra hircus) in rural KwaZulu-Natal, South Africa. Front Microbiol. 15:1349163. 2024. |
|
|
Gomez-Buendia A, Alvarez J, Bezos J, Mourelo J, Amado J, Saez JL, de Juan L, Romero B. Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds. Front Vet Sci. 11:1361788. 2024. |
|
|
Patnaik N, Dey RJ. Label-Free Citrate-Stabilized Silver Nanoparticles-Based, Highly Sensitive, Cost-Effective, and Rapid Visual Method for the Differential Detection of Mycobacterium tuberculosis and Mycobacterium bovis. ACS Infect Dis. Epub ahead of print. 2023. |
|
|
Vera-Salmoral E, Gómez-Laguna J, Galán-Relaño Á, Ruedas-Torres I, Carrasco L, Luque I, Huerta B, Sánchez-Carvajal JM. Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex. Microbiol Spectr. 11(5):e0034823. 2023. |
|
|
Rodríguez-Hernández P, Cardador MJ, Ríos-Reina R, Sánchez-Carvajal JM, Galán-Relaño Á, Jurado-Martos F, Luque I, Arce L, Gómez-Laguna J, Rodríguez-Estévez V. Detection of Mycobacterium tuberculosis complex field infections in cattle using fecal volatile organic compound analysis through gas chromatography-ion mobility spectrometry combined with chemometrics. Microbiol Spectr. 11(5):e0174323. 2023. |
| Immunology | |
|
|
Blanco FC, Onnainty R, Marini MR, Klepp LI, García EA, Vazquez CL, Canal A, Granero G, Bigi F. M72 Fusion Proteins in Nanocapsules Enhance BCG Efficacy Against Bovine Tuberculosis in a Mouse Model. Pathogens. 2025 Jun 16;14(6):592. |
|
|
Lakew M, Conlan AJK, Tadesse B, Srinivasan S, Yalew B, Benti T, Olani A, Kinfe G, Ashagrie T, Abebe A, Fromsa A, Abdela MG, Bayissa B, Gebre S, Mihret A, Mekonnen GA, Ameni G, Ashenafi H, Wood JLN, Gumi B, Kapur V. Comparative performance and age dependence of tuberculin and defined antigen bovine tuberculosis skin tests assessed with Bayesian latent class analysis. Sci Rep. 2025 Jun 5;15(1):19728. |
|
|
Chileshe J, Shury T, Chen JM. Optimization and validation of ELISAs for interferon-gamma determination in bison. J Vet Diagn Invest. 2025 Jun 3:10406387251344567. |
|
|
Holder T, Robinson N, Jones GJ. The Impact of Bacillus Calmette-Guérin Vaccination and Mycobacterium bovis Infection on Diagnostic Antibody Tests for Mycobacterial Infections. Vaccines (Basel). 2025 May 28;13(6):578. |
|
|
Agulló-Ros I, Burucúa MM, Cheuquepán FA, Domínguez M, Sevilla IA, Martínez R, Plá N, Risalde MA, Marin MS. Heat-inactivated Mycobacterium bovis and P22PI protein immunocomplex: Two candidates for use as immunostimulants of innate immune response. Vet Microbiol. 2025 Jun;305:110527. doi: 10.1016/j.vetmic.2025.110527. |
|
|
Bhaskar V, Kumar R, Praharaj MR, Gandham S, Maity HK, Sarkar U, Dey B. A bovine pulmosphere model and multiomics reveal early host response signature in tuberculosis. Commun Biol. 2025 Apr 4;8(1):559. |
|
|
Hayton A, O´Brien A, Adler A, Cutler K, Clarke J, Shaw DJ, Watt NJ, Harkiss GD. Diagnostic performance of the Enferplex Bovine TB antibody test using bulk tank milk samples from dairy cattle. J Dairy Sci. 2025 Mar 24:S0022-0302(25)00163-8. |
|
|
Kumar R, Gandham S, Maity HK, Sarkar U, Dey B. Transcriptome profiling in an in vitro peripheral blood mononuclear cell - Mycobacterium tuberculosis infection model reveals breed-specific immune gene signatures potentially associated with tuberculosis susceptibility in cattle. Int J Biol Macromol. 2025 Feb 20;306(Pt 1):141282. |
|
|
Díaz-Otero F, Jaramillo-Meza L, Manzo-Sandoval A, Olguín-Alor R, Diosdado-Vargas F. Comparative longitudinal analysis of T lymphocyte subpopulations in calves vaccinated with different doses of BCG-Phipps or with culture filtrate protein extract of Mycobacterium bovis in a natural transmission setting. BMC Vet Res. 2025 Feb 19;21(1):78. |
|
|
Fromsa A, Conlan AJK, Srinivasan S, Gumi B, Bedada W, Zeleke M, Worku D, Lakew M, Tadesse B, Bayissa B, Sirak A, Abdela MG, Mekonnen GA, Chibssa T, Veerasami M, Jones GJ, Vordermeier HM, Juleff N, Wood JLN, Ameni G, Kapur V. Comparative performance of tuberculin and defined-antigen cocktails for detecting bovine tuberculosis in BCG-vaccinated cattle in natural settings. Sci Rep. 2025 Feb 7;15(1):4564. |
| Molecular characterisation | |
|
|
Zhou H, Wu W, Zhang Q, Zhang T, Jiang S, Liu H, Ma Y, Chang L, Xie Y, Zhu J, Zhou D, Zhang Y, Xu P. Quantitative Proteome Analysis of Plasma Extracellular Vesicles Identifies Three Proteins with Potential Diagnostic Value for Mycobacterium bovis Infection in Cows. J Proteome Res. 2025 Jun 6;24(6):2926-2938. |
|
|
Szacawa E, Kozieł N, Brzezińska S, Augustynowicz-Kopeć E, Weiner M, Szulowski K, Krajewska-Wędzina M. Laboratory Diagnosis of Animal Tuberculosis in Tracing Interspecies Transmission of Mycobacterium bovis. Pathogens. 2025 May 7;14(5):459. |
|
|
Wei Y, Yuan M, Zhang Y, Gao Y. The 2SP Site Mutation in the Bovine Natural Resistance-Associated Macrophage 1 Promoter Exhibits Antituberculosis Potential. Int J Mol Sci. 2025 Apr 29;26(9):4229. |
|
|
Lorente-Leal V, Pozo P, Bezos J, Collado S, Vicente J, Stuber T, Álvarez J, de Juan L, Romero B. Revisiting Mycobacterium bovis SB0121 genetic diversity reflects the complexity behind bovine tuberculosis persistence in Spain. Prev Vet Med. 2025 Jun;239:106519. |
|
|
Sandhu P, Nunez-Garcia J, Berg S, Wheeler J, Dale J, Upton P, Gibbens J, Hewinson RG, Downs SH, Ellis RJ, Palkopoulou E. Enhanced analysis of the genomic diversity of Mycobacterium bovis in Great Britain to aid control of bovine tuberculosis. Front Microbiol. 2025 Mar 25;16:1515906. |
|
|
O´Grady JF, McHugo GP, Ward JA, Hall TJ, Faherty O´Donnell SL, Correia CN, Browne JA, McDonald M, Gormley E, Riggio V, Prendergast JGD, Clark EL, Pausch H, Meade KG, Gormley IC, Gordon SV, MacHugh DE. Integrative genomics sheds light on the immunogenetics of tuberculosis in cattle. Commun Biol. 2025 Mar 24;8(1):479. |
|
|
Mohammed T, Desta F, Wondale B, Zewude A, Mamo G, Khalifa HO, Bayissa B, Ameni G. Epidemiology of Mycobacterium tuberculosis complex infections in cattle and humans in the remote pastoral settings of southern Ethiopia. Front Vet Sci. 2025 Mar 19;12:1551710. |
|
|
Zaba MY, Neway S, Farrell D, Denion E, Perets V, Tilahun M, Bobosha K, Cassidy JP, Asmare A, Gordon SV; WHO; WHO. Isolation and genetic analysis of mycobacteria from suspect tuberculous lesions in slaughtered cattle from Wolaita, Ethiopia. Access Microbiol. 2025 Mar 18;7(3):000915.v3. |
|
|
Woldemariam T, Mohammed T, Zewude A, Chanyalew M, Khalifa HO, Mamo G, Ameni G. Zoonotic transmission of the Mycobacterium tuberculosis complex between cattle and humans in Central Ethiopia. Front Vet Sci. 2025 Mar 10;12:1527279. |
|
|
Matthews MC, Cooke DM, Kerr TJ, Loxton AG, Warren RM, Ghielmetti G, Streicher EM, Witte CS, Miller MA, Goosen WJ. Evidence of Mycobacterium bovis DNA in shared water sources at livestock-wildlife-human interfaces in KwaZulu-Natal, South Africa. Front Vet Sci. 2025 Feb 28;12:1483162. |
| Others | |
|
|
George BM, Duncan D, Waller E, Marriott E, Payne MC, Withenshaw S, Brouwer A, Harris KA, May HE, Avigad R. Bovine TB infection status in cattle in Great Britain in 2023. Vet Rec. 2025 Jun 21;196(12):e5696. |
|
|
Palmer MV, Jones DE, Bockenstedt NJ, Boggiatto PM. Tertiary lymphoid structures in pulmonary granulomas of cattle experimentally infected with aerosolized Mycobacterium bovis. BMC Vet Res. 2025 Jun 5;21(1):403. |
|
|
Peruzy MF, Houf K, Ambrosio RL, Sorlini GML, Ceccarelli M, Tomei F, Murru N. Evaluating remote post-mortem inspection in bovines: Challenges, limitations, and future potential in assessing post-mortem outcomes in cattle offal. Food Res Int. 2025 Aug;214:116644. |
|
|
Commandeur S, van der Most M, Koomen J, van Keulen L, Dinkla A, Luinenburg X, Escher M, Jacobs P, Keur I, Grinwis GCM, Weerts E, Broens EM, Anthony R, Agterveld MK, Rebel K, Huisman E, Heijne M, Koets A. Mycobacterium bovis infected domestic cats in an officially bovine tuberculosis free country resulting in human infection. One Health. 2025 Apr 21;20:101048. |
|
|
Holmes EL, O´Hagan MJH, Menzies FD, Byrne AW, McBride KR, McCormick CM, Scantlebury DM, Reid N. Landscape as a Shared Space for Badgers and Cattle: Insights Into Indirect Contact and Bovine Tuberculosis Transmission Risk. Ecol Evol. 2025 Apr 15;15(4):e71114. |
|
|
Balks E, Trösemeier JH, Mühlebach MD, Reuter A. Mass spectrometry analysis of bovine tuberculins - 3R potential in batch potency testing. Biologicals. 2025 May;90:101825. |
|
|
Powell SM, Dessi N, Bennett M, Wang B, Robertson A, Waller E, Smith GC, Delahay RJ. Tuberculosis in found dead badgers at the edge of the expanding bovine tuberculosis epidemic. Sci Rep. 2025 Mar 27;15(1):10547. |
|
|
Lakew M, Tadesse B, Bedada W, Wakjira BS, Mekonnen GA, Chibssa TR, Ashenafi H, Ameni G, Conlan AJK, Bakker D, Gumi B, Kapur V. Early calf segregation enables development of bovine tuberculosis-free replacement stock in a highly infected dairy herd: a preliminary study in Ethiopia. Front Vet Sci. 2025 Mar 19;12:1551065. |
|
|
Jinks RC, Lesellier S, Smith F, Menzies FD, Ashford RT, Waring L, Dave D, Anderson P, Stringer LA, Pascual-Linaza AV, Corbett D, Thompson S, Arnold ME. Evaluating sensitivity and specificity of the DPP Vet TB assay in badgers using Bayesian latent class models. PLoS One. 2025 Mar 4;20(3):e0313825. |
|
|
Robertson A, Chambers MA, Smith GC, Delahay RJ, McDonald RA, Brotherton PNM. Can badger vaccination contribute to bovine TB control? A narrative review of the evidence. Prev Vet Med. 2025 May;238:106464. |
