Publications Database is an online system designed to list the new scientific publications related to diagnostic techniques (culture, extraction & identification, molecular characterisation and interferon gamma detection).
Periodic searches will be carried out in scientific databases and an e-mail will be sent automatically to all NRLs/EC with the latest updates. A link to the publications will be available.
Last Publications
Culture
09/12/2024
Lyu J, Narum DE, Baldwin SL, Larsen SE, Bai X, Griffith DE, Dartois V, Naidoo T, Steyn AJC, Coler RN, Chan ED. Understanding the development of tuberculous granulomas: insights into host protection and pathogenesis, a review in humans and animals. Front Immunol. 2024 Dec 9;15:1427559
18/10/2024
Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata. BMC Vet Res. 2024 Oct 18;20(1):478.
10/09/2024
Liebler-Tenorio EM, Wedlich N, Figl J, Köhler H, Ulrich R, Schröder C, Rissmann M, Grode L, Kaufmann SHE, Menge C. Challenge Dose Titration in a <i>Mycobacterium bovis</i> Infection Model in Goats. Int J Mol Sci. 2024 Sep 10;25(18):9799.
07/08/2024
Palmer MV, Kanipe C, Hwang S, Thacker TC, Lehman KA, Ledesma NA, Gustafson KK, Boggiatto PM. Pathogen Detection in Early Phases of Experimental Bovine Tuberculosis. Vet Sci. 2024 Aug 7;11(8):357.
30/07/2024
hielmetti G, Kerr TJ, Bernitz N, Mhlophe SK, Streicher E, Loxton AG, Warren RM, Miller MA, Goosen WJ. Insights into mycobacteriome composition in Mycobacterium bovis-infected African buffalo (Syncerus caffer) tissue samples. Sci Rep. 2024 Jul 30;14(1):17537.
01/03/2024
Ferrari S, Zanoni M, Mangeli A, Pigoli C, D´Incau M, Alborali GL, Pacciarini ML, Boniotti MB. Bacteriological culture and direct PCR for detecting the Mycobacterium tuberculosis complex in the Italian eradication campaign: a decade of experience at the National Reference Laboratory. J Appl Microbiol. 135(3):lxae064. 2024.
03/01/2024
Dwyer R, Witte C, Buss P, Warren R, Miller M, Goosen W. Antemortem detection of Mycobacterium bovis in nasal swabs from African rhinoceros. Sci Rep. 14(1):357. 2024.
29/12/2021
Didkowska A, Orłowska B, Krajewska-Wędzina M, Augustynowicz-Kopeć E, Brzezińska S, Żygowska M, Wiśniewski J, Kaczor S, Welz M, Olech W, Anusz K. Microbiological and molecular monitoring for bovine tuberculosis in the Polish population of European bison (Bison bonasus). Ann Agric Environ Med. 28(4):575-578. 2021.
20/10/2021
Ejo M, Haile B, Tariku T, Nigatu S, Kebede E, Bitew AB, Demessie Y, Getaneh G, Alebie A, Girma M, Ota F, Nuru A. Bacteriological and molecular characterization of Mycobacterium bovis isolates from tuberculous lesions collected among slaughtered cattle, Northwest Ethiopia. BMC Microbiol. 21(1):286. 2021.
01/04/2020
Hlokwe TM, Mogano RM. Utility of xpert® MTB/RIF ultra assay in the rapid diagnosis of bovine tuberculosis in wildlife and livestock animals from South Africa. Prev Vet Med. 2020 Apr;177:104980. 2020.
Extraction & identification
13/06/2024
Zhu Y, Feng Z, Xu Y, Luo S, Zhang R, Shi X, Wu X, Zhang H. Rapid detection of Mycobacterium tuberculosis based on cyp141 via real-time fluorescence loop-mediated isothermal amplification (cyp141-RealAmp). Front Cell Infect Microbiol. 14:1349063. 2024.
23/05/2024
Liu Q, Yang M, Zhang H, Ma W, Fu X, Li H, Gao S. A colorimetric tandem combination of CRISPR/Cas12a with dual functional hybridization chain reaction for ultra-sensitive detection of Mycobacterium bovis. Anal Methods. 16(20):3220-3230. 2024.
20/05/2024
Encinas M, Ferrara Muñiz X, Sammarruco RA, Ruiz Menna V, Garro CJ, Delgado F, Macías A, Magnano G, Zumárraga MJ, Garbaccio SG, Eirin ME. Limited usefulness of the IS6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis. Front Vet Sci. 11:1359205. 2024.
01/05/2024
Mabe L, Muthevhuli M, Thekisoe O, Suleman E. Accuracy of molecular diagnostic assays for detection of Mycobacterium bovis: A systematic review and meta-analysis. Prev Vet Med. 226:106190. 2024.
26/02/2024
Sánchez-Carvajal JM, Vera-Salmoral E, Huerta B, Galán-Relaño Á, Ruedas-Torres I, Larenas-Muñoz F, Luque I, Carrasco L, Gómez-Laguna J. Droplet digital PCR as alternative to microbiological culture for Mycobacterium tuberculosis complex detection in bovine lymph node tissue samples. Front Cell Infect Microbiol. 14:1349999. 2024.
14/02/2024
Cooke DM, Clarke C, Kerr TJ, Warren RM, Witte C, Miller MA, Goosen WJ. Detection of Mycobacterium bovis in nasal swabs from communal goats (Capra hircus) in rural KwaZulu-Natal, South Africa. Front Microbiol. 15:1349163. 2024.
31/01/2024
Gomez-Buendia A, Alvarez J, Bezos J, Mourelo J, Amado J, Saez JL, de Juan L, Romero B. Non-tuberculous mycobacteria: occurrence in skin test cattle reactors from official tuberculosis-free herds. Front Vet Sci. 11:1361788. 2024.
19/12/2023
Patnaik N, Dey RJ. Label-Free Citrate-Stabilized Silver Nanoparticles-Based, Highly Sensitive, Cost-Effective, and Rapid Visual Method for the Differential Detection of Mycobacterium tuberculosis and Mycobacterium bovis. ACS Infect Dis. Epub ahead of print. 2023.
14/09/2023
Vera-Salmoral E, Gómez-Laguna J, Galán-Relaño Á, Ruedas-Torres I, Carrasco L, Luque I, Huerta B, Sánchez-Carvajal JM. Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex. Microbiol Spectr. 11(5):e0034823. 2023.
13/09/2023
Rodríguez-Hernández P, Cardador MJ, Ríos-Reina R, Sánchez-Carvajal JM, Galán-Relaño Á, Jurado-Martos F, Luque I, Arce L, Gómez-Laguna J, Rodríguez-Estévez V. Detection of Mycobacterium tuberculosis complex field infections in cattle using fecal volatile organic compound analysis through gas chromatography-ion mobility spectrometry combined with chemometrics. Microbiol Spectr. 11(5):e0174323. 2023.
Immunology
24/12/2024
Franzoni G, Signorelli F, Mazzone P, Donniacuo A, De Matteis G, Grandoni F, Schiavo L, Zinellu S, Dei Giudici S, Bezos J, De Carlo E, Galiero G, Napolitano F, Martucciello A. Cytokines as potential biomarkers for the diagnosis of Mycobacterium bovis infection in Mediterranean buffaloes (Bubalus bubalis). Front Vet Sci. 2024 Dec 24;11:1512571.
29/11/2024
Agulló-Ros I, Vaz-Rodrigues R, Domínguez M, Roy Á, Ortega J, Moreno I, Bezos J, Domínguez L, Fernández de Mera IG, Risalde MA. Immunological mechanisms involved in the protection against development of pulmonary tuberculosis in naturally infected goats. Vet Microbiol. 2025 Jan;300:110320.
27/11/2024
Sterle HM, Putz EJ, Olsen SC, Boggiatto PM. Induction of CD4 T cell memory responses following BCG vaccination in cattle. Front Vet Sci. 2024 Nov 27;11:1491424.
19/11/2024
Ortega J, Agulló-Ros I, Roy A, Moreno I, Gómez-Buendía A, Romero B, Ferreras-Colino E, de Juan L, Domínguez M, Domínguez L, Risalde MA and Bezos J. A high titer antibody response against P22 protein immunocomplex is not correlated with protection in naturally tuberculosis-infected goats. Vet Q. 2024 Dec;44(1):16-30.
17/10/2024
Ferrara Muñiz X, García E, Blanco FC, Garbaccio S, Garro C, Zumárraga M, Dellagostin O, Trangoni M, Marfil MJ, Bianco MV, Abdala A, Revelli J, Bergamasco M, Soutullo A, Marini R, Rocha RV, Sánchez A, Bigi F, Canal AM, Eirin ME, Cataldi AA. Field Trial with Vaccine Candidates Against Bovine Tuberculosis Among Likely Infected Cattle in a Natural Transmission Setting. Vaccines (Basel). 2024 Oct 17;12(10):1173.
09/10/2024
Klepp LI, Blanco FC, Bigi MM, Vázquez CL, García EA, Sabio Y García J, Bigi F. B Cell and Antibody Responses in Bovine Tuberculosis. Antibodies (Basel). 2024 Oct 9;13(4):84.
27/09/2024
Mason PS, Risalde MA, Gortázar C, Garrido JM, Thomas DA, Al Dulayymi JR, Baird MS. Early antibody responses to lipid antigens in red deer infected with Mycobacterium bovis. Vet Microbiol. 2024;298:110269
08/09/2024
Gomez-Buendia A, Ortega J, Diez-Guerrier A, Rendahl A, Saez JL, Bezos J, Romero B, Alvarez J. Evaluating the ability of non-tuberculous mycobacteria to induce non-specific reactions in bovine tuberculosis diagnostic tests in guinea pigs and cattle. Vet Microbiol. 2024 Sep 8;298:110250.
06/09/2024
Wang C, Jiang Y, Yang Z, Xu H, Khalid AK, Iftakhar T, Peng Y, Lu L, Zhang L, Bermudez L, Guo A, Chen Y. Host factor <i>RBMX2</i> promotes epithelial cell apoptosis by downregulating APAF-1´s Retention Intron after <i>Mycobacterium bovis</i> infection.Front Immunol. 2024 Sep 6;15:1431207.
23/07/2024
Yang H, Lei X, Chai S, Su G, Du L. From pathogenesis to antigens: the key to shaping the future of TB vaccines. Front Immunol. 2024 Jul 23;15:1440935.
Molecular characterisation
19/12/2024
Lorente-Leal V, Gomez-Buendia A, Gutiérrez-Tobaruela A, de Juan L, Bezos J, Romero B. Shaken, not stirred: magnetic bead DNA extraction as a rapid and effective method for the scaling up of bovine tuberculosis diagnosis. BMC Vet Res. 2024 Dec 19;20(1):568.
01/11/2024
Soliman NS, Soliman MS, Khairat SM, Gad MA, Shawky S, Elkholy AA. Genetic diversities and drug resistance in Mycobacterium bovis isolates from zoonotic tuberculosis using whole genome sequencing. BMC Genomics. 2024 Nov 1;25(1):1024.
29/10/2024
Hossain MM, Rahman MM, Rahman MM, Hossain H, Begum R, Chowdhury MSR, Islam MR, Uddin MB. Molecular and Serological Detection of Mycobacterium tuberculosis in Dairy Cattle From Sylhet, Bangladesh: Implications for Zoonotic Transmission. Vet Med Int. 2024 Oct 30;2024:3653654.
09/10/2024
Fromsa A, Conlan AJK, Srinivasan S, Zeleke M, Worku D, Lakew M, Abdela MG, Bahiru G, Wood JLN, Bakker D, Gumi B, Ameni G, Kapur V. Bovine tuberculosis in Central Ethiopian slaughterhouses and the identification of causative mycobacteria by multiplex real-time PCR. BMC Microbiol. 2024 Oct 9;24(1):394.
22/08/2024
Pereira AC, Pérez de Val B, Cunha MV. Phylogenetic analysis of Mycobacterium caprae highlights past and present epidemiological links at the Iberian Peninsula scale. Microbes Infect. 2024 Aug 22:105405.
06/08/2024
Bhat SA, Parveen A, Gormley E, Meade KG. Extensive differential DNA methylation between tuberculosis skin test positive and skin test negative cattle.BMC Genomics. 2024 Aug 6;25(1):762.
21/05/2024
Pereira AC, Lourenço J, Themudo G, Botelho A, Cunha MV. Population structure and history of Mycobacterium bovis European 3 clonal complex reveal transmission across ecological corridors of unrecognized importance in Portugal. Microbiol Spectr. 12(7):e0382923. 2024.
29/04/2024
Pereira AC, Pinto D, Cunha MV. First time whole genome sequencing of Mycobacterium bovis from the environment supports transmission at the animal-environment interface. J Hazard Mater. 472:134473. 2024.
04/04/2024
Refaya AK, Vetrivel U, Palaniyandi K. Genomic Characterization of IS6110 Insertions in Mycobacterium orygis. Evol Bioinform Online. 20:11769343241240558. 2024.
03/03/2024
Goosen WJ, Moodley S, Ghielmetti G, Moosa Y, Zulu T, Smit T; Vukuzazi Team; Kleynhans L, Kerr TJ, Streicher EM, Hanekom WA, Warren RM, Wong EB, Miller MA. Identification and molecular characterization of Mycobacterium bovis DNA in GeneXpert® MTB/RIF ultra-positive, culture-negative sputum from a rural community in South Africa. One Health. 18:100702. 2024.
Others
22/12/2024
Xu L, Li S, Li H, Pan H, Li S, Yang Y, Jiao Y, Lan F, Chen S, Chen Q, Du L, Man C, Wang F, Gao H. Predicting Tuberculosis Risk in Cattle, Buffaloes, Sheep, and Goats in China Based on Air Pollutants and Meteorological Factors. Animals (Basel). 2024 Dec 22;14(24):3704.
20/12/2024
More SJ. Eradication of bovine tuberculosis in Ireland: is it a case of now or never? Ir Vet J. 2024 Dec 20;77(1):22.
28/11/2024
Chang Y, Widgren S, de Jong MCM, Tratalos JA, More SJ, Hartemink N. Evaluating the effectiveness of badger vaccination combined with cattle test-and-removal in managing Bovine Tuberculosis: Insights from a two-host and multi-route transmission model. Prev Vet Med. 2024 Nov 28;235:106386.
09/11/2024
Wiseman J, Cassidy JP, Gormley E. The problem that residual Mycobacterium bovis infection poses for the eradication of bovine tuberculosis. Vet J. 2024 Dec;308:106266.
01/11/2024
Seery S, Schroeder P, Galloway T, Abernethy D, Hewinson G. Bovine tuberculosis trends in Wales between 2010 and 2021. Vet Rec. 2024 Nov 2;195(9):e4600.
25/09/2024
Didkowska A, Krajewska-Wędzina M, Miller M, Bochniarz M, Kozińska M, Szacawa E, Tracz A, Weiner M, Brzezińska S, Augustynowicz-Kopeć E, Anusz K, Nowakiewicz A. <i>Mycobacterium caprae</i> tuberculosis in a captive lion in Ukraine - Case Report.Ann Agric Environ Med. 2024 Sep 25;31(3):455-459.
31/08/2024
Jabeen C, Mushtaq MH, Khan JA, Umbreen G, Saeed MS, Sadiq S, Maqsood R, Bin Rashid H, Asif M, Iqbal K, Chaudhry M. A retrospective analysis of tuberculosis in livestock farmers in Lahore district, Pakistan. J Infect Dev Ctries. 2024 Aug 31;18(8):1249-1257.
26/08/2024
Tsasse MAF, Dilonga Meriki H, Nana Djeunga HC, Ngwa MA, Tatsilong Pambou HO, Dongmo R, Nguessi O, Kamgno J, Akoachere JTK, Nguipdop-Djomo P. Exposure to bovine livestock and latent tuberculosis infection in children: Investigating the zoonotic tuberculosis potential in a large urban and peri-urban area of Cameroon. PLOS Glob Public Health. 2024 Aug 26;4(8):e0003669.
14/08/2024
Van Der Zwan A, Campbell PT, Shi N, De Bortoli N, Villanueva-Cabezas JP. Systematic review of knowledge, attitudes, and practices of dairy farmers and consumers towards bovine tuberculosis in low- and middle-income countries. Prev Vet Med. 2024 Nov;232:106314.
03/08/2024
Jones T. TB testing and transmission. Vet Rec. 2024 Aug 3;195(3):114-115.