Home \ Research \ Scientific publications \


Antibiotic Resistance Genes in Freshwater Trout Farms in a Watershed in Chile

Investigation article published in Journal of Environmental Quality

In Press

Point sources such as wastewater treatment plants, terrestrial agriculture, and aquaculture may release antibiotic residues, antibiotic resistant bacteria, and antibiotic resistance genes (ARGs) into aquatic ecosystems. However, there is a lack of quantitative studies attributing environmental ARG abundance to specific sources. The goal of this study was to evaluate the role of freshwater trout farms in the release and dissemination of ARGs into the environment. Sediment samples upstream and downstream from five rainbow trout farms were collected over time in southern Chile. A microfluidic quantitative polymerase chain reaction approach was used to quantify an ARG array covering different mechanisms of resistance, and data were analyzed using principal component analysis (PCA) and linear mixed regression models. Surveys were also conducted to obtain information about management practices, including antibiotic use, at the farms. Florfenicol and oxytetracycline were used at these farms, although at different rates. A total of 93 samples were analyzed. In the PCA, strB, sul1, sul2, qacG, tet(A), tet(B), tet(C), tet(W), and floR grouped together. A statistically significant increase in abundance of qacG, strB, sul1, and several tet genes was found downstream from the farms compared with upstream sites, and retention ponds had the highest ARG abundance at each site. Antibiotic resistance gene levels returned to baseline at an average distance of 132.7 m downstream from the farms. Although results from this study indicate an influence of trout farms on the presence of ARGs in the immediate environment, the extent of their contribution to ARG dissemination is unknown and deserves further investigation




Bueno I., Travis D., Gonzalez-Rocha G., Alvarez J., Lima C., Garcia-Benitez C., Phelps NBD., Wass B., Johnson TJ., Zhang Q., Ishii S. and Singer RS.




See this article
Antibiotic Resistance Genes in Freshwater Trout Farms in a Watershed in Chile



Participants:

University of MinnesotaDepartment of Veterinary and Biomedical Sciences. Department of Veterinary Population Medicine. College of Veterinary Medicine. University of Minnesota (UMM).

Universidad de ConcepciónDepartamento de Microbiología. Facultad de Ciencias Biológicas. Universidad de Concepción.

Universidad de ConcepciónLaboratorio de Investigación en Agentes Antibacterianos (LIAA). Universidad de Concepción.

Millennium Nucleus on Interdisciplinary Approach to Antimicrobial ResistanceMillennium Nucleus on Interdisciplinary Approach to Antimicrobial Resistance.

Universidad ComplutenseCentro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Piscicola Entre RiosPiscicola Entre Rios.

University of MinnesotaWildlife and Conservation Biology. University of Minnesota (UMM).

University of MinnesotaBioTechnology Institute. University of Minnesota (UMM).

University of MinnesotaDepartment of Soil, Water, and Climate. University of Minnesota (UMM).







Journal of Environmental Quality
FACTOR YEAR Q
2.579 2018

ISSN: 1537-2537



TITLE: Antibiotic Resistance Genes in Freshwater Trout Farms in a Watershed in Chile


JOURNAL: J. Environ. Qual.


PUBLISHER: American Society of Agronomy


AUTHORS: Bueno I., Travis D., Gonzalez-Rocha G., Alvarez J., Lima C., Garcia-Benitez C., Phelps NBD., Wass B., Johnson TJ., Zhang Q., Ishii S. and Singer RS.


VISAVET PARTICIPANTS


Julio Álvarez Sánchez

DOI: https://doi.org/ 10.2134/jeq2018.12.0431


CITE THIS PUBLICATION:

Bueno I., Travis D., Gonzalez-Rocha G., Alvarez J., Lima C., Garcia-Benitez C., Phelps NBD., Wass B., Johnson TJ., Zhang Q., Ishii S. and Singer RS. Antibiotic Resistance Genes in Freshwater Trout Farms in a Watershed in Chile. Journal of Environmental Quality. In Press. (A). ISSN: 1537-2537. DOI: 10.2134/jeq2018.12.0431