Research

ONE HEALTH EJP granted by the European Commission

VISAVET-UCM participates in this Horizon 2020 project with a European grant of 45 million euros

The One Health concept recognizes that the human health is tightly connected to the health of animals and the environment, i.e. that animal feed, human food, animal and human health and environmental contamination are closely linked.… Leer más

Insights into a novel blaKPC-2 -encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain

Frontiers in Microbiology publish this investigation article

Untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. However, its role in the spread of antibiotic resistances in the human population remains poorly investigated. We used whole genome sequencing (WGS) to analyze 25 KPC-2-producing Enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical Citrobacter freundii isolates from a tertiary hospital in the same collection area in Spain. We detected a common, recently described, IncP-6 plasmid carrying the gene blaKPC… Leer más

Epidemiology of Q fever in domestic ruminants in the central zone of the Iberian peninsula

PhD Thesis defense by Teresa García-Seco Romero at the Faculty of Veterinary Medicine of de Complutense University of Madrid

Coxiella burnetii is the aetiological agent of Q fever, a worldwide distributed zoonosis whose main reservoirs and source of infection for humans are domestic ruminants. The main clinical sign associated with C. burnetii infection is late-term abortion in small ruminants, and fertility problems in cattle. However, the impact of Q fever in animal production has been traditionally considered limited since infection is more commonly unapparent. Still, its ability to cause important clinical outbreaks has become evident in the last years, so that nowadays it is considered one of the main causes of… Leer más

New advances in the diagnosis of diseases in marine mammals

Mar Melero Asensio defended the PhD Thesis at the Faculty of Veterinary Medicine of de Complutense University of Madrid

Marine mammals have long been considered good indicators of marine ecosystem health because they have long lifespans; blubber stores that can serve as depots for chemicals and toxins and some species occupy a high trophic level. In addition to this, many species reside along coasts; human activities like fishing and maritime traffic affect them, and they are vulnerable to many infectious diseases. Some of these have implications for human public health and others may serve as indicators of environmental distress syndrome. Furthermore, marine mammals are very charismatic animals, and thus provi… Leer más

Antibody detection tests improve the sensitivity of tuberculosis diagnosis in cattle

Investigation published in Research in Veterinary Science

We evaluated the sensitivity (Se) of the single cervical intradermal tuberculin (SIT) test, two interferon-gamma (IFN-γ) assays and three different antibody detection techniques for bovine tuberculosis (bTB) diagnosis in 131 mixed beef breed cattle. The results of the diagnostic techniques performed over the whole herd, and over the animals confirmed as infected based on the presence of lesions compatible with the disease and/or M. bovis isolation were compared to determine apparent prevalence (AP) and Se. The Se of the SIT test (severe interpretation) was 63.7% (95% CI, 54.54–72.00), wh… Leer más

A novel mutation in pmrB mediates colistin resistance during therapy of Acinetobacter baumannii

International Journal of Antimicrobial Agents publish this investigation article

Acinetobacter baumannii is a highly versatile nosocomial pathogen. Multidrug resistance among A. baumannii isolates led to the use of colistin, subsequently giving rise to colistin-resistant strains. In this study, the genetic and phenotypic profiles of two colistin-resistant A. baumannii isolates were investigated. Two A. baumannii isolates were obtained from Patient 1 (C071 and C440) and three isolates were obtained from Patient 2 (C080, C314 and C428). Susceptibility profiles were determined by VITEK®2 and Etest. Clonality was determined by RAPD analysis and trilocus multiplex PCR. The pmrC… Leer más

First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis

Journal of Equine Veterinary Science publish this investigation article

Strangles is one of the most frequently diagnosed equine respiratory infectious diseases in the world. It is caused by Streptococcus equi subsp. equi (S. equi), and it is an acute infection characterized by pyrexia, nasal discharge, pharyngitis, and abscessation of lymph nodes. Frequently, healthy horses might continue to harbor S. equi after clinical recovery. Although the genetic distance between S. equi isolates is short, strains can be differentiated by pulsed-field gel electrophoresis (PFGE) and single locus sequence typing for epidemiological studies. The aim of this study was to charact… Leer más

Human influence and biotic homogenization drive the distribution of Escherichia coli virulence genes in natural habitats

Investigation published in MicrobiologyOpen

Cattle are the main reservoirs for Shiga-toxin-producing Escherichia coli (STEC), the only known zoonotic intestinal E. coli pathotype. However, there are other intestinal pathotypes that can cause disease in humans, whose presence has been seldom investigated. Thus, our aim was to identify the effects of anthropic pressure and of wild and domestic ungulate abundance on the distribution and diversity of the main human E. coli pathotypes and nine of their representative virulence genes (VGs). We used a quantitative real-time PCR (qPCR) for the direct detection and quantification of the genus-sp… Leer más

Enterococcus, Salmonella y H2S: Control Remoto de la Resistencia a Antibioticos

Daniel Thomas López defended the PhD Thesis at the Faculty of Veterinary Medicine of de Complutense University of Madrid

Highly Tigecycline-Resistant Klebsiella pneumoniae Sequence Type 11 (ST11) and ST147 Isolates from Companion Animals

Investigation published in Antimicrobial Agents and Chemotherapy

In this study we have characterized two tigecycline resistant Klebsiella pneumoniae clones isolated from dog urine samples. The isolates were genetically unrelated, belonging to sequence types ST11 and ST147, both classically related to human isolates. To the best of our knowledge, this is the first identification of tigecycline resistant isolates from animals. We unveil the worrisome circulation among animals of bacterial clones resistant to this last resort antibiotic… Leer más

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- Investigadores de la Universidad Complutense localizan en perros un patógeno humano resistente a un antibiótico de último recurso - ecoaula.eleconomista.es

- Equipo de la UCM halla bacteria resistente al antibiótico de último recurso - elconfidencial.com

- Equipo de la UCM halla bacteria resistente al antibiótico de último recurso - abc.es

- Equipo de la UCM halla bacteria resistente al antibiótico de último recurso - lavanguardia.com

- Resistanace to antibiotic of las resort found in human pathogens infecting dogs - asm.org