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January 1st, 2018

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The relevance of host overcrowding in wildlife epidemiology: A new spatially explicit aggregation index

Ecological Indicators publish this investigation article

Species distribution and population abundance are keystone patterns in ecology, and currently also in epidemiology. The aggregation of individuals in the population is closely related to distribution and abundance, but they are not totally equivalent patterns. Despite the great efforts made in recent decades to harmonise the sampling protocols used to collect distribution and abundance data, studies regarding the development and testing of aggregation indices are scarce, even when individuals’ aggregation is quite relevant and necessary for the design of effective wildlife management policies.… Leer más

January 1st, 2018

Vía publiDB

Recoding of synonymous genes to expand evolutionary landscapes requires control of secondary structure affecting translation

Biotechnology and bioengineering publish this investigation article

Synthetic DNA design needs to harness the many information layers embedded in a DNA string. We previously developed the Evolutionary Landscape Painter (ELP), an algorithm that exploits the degeneracy of the code to increase protein evolvability. Here, we have used ELP to recode the integron integrase gene (intI1) in two alternative alleles. Although synonymous, both alleles yielded less IntI1 protein and were less active in recombination assays than intI1. We spliced the three alleles and mapped the activity decrease to the beginning of alternative sequences. Mfold predicted the presence of mo… Leer más

December 16th, 2017

Vía publiDB

BMC Veterinary Research

Genetic diversity assessment of Tunisian Mycobacterium bovis population isolated from cattle

BMC Veterinary Research publish this investigation article

Background The genetic diversity of M. bovis in Tunisia is still underestimated despite the implementation of an eradication program. The lack of data about spatial distribution of the M. bovis population hinders the control of bovine tuberculosis (bTB) progress. This study represents the largest molecular analysis of M. bovis isolates in Tunisia. It is aimed to upgrade the understanding of bTB epidemiology and the geographical distribution of the infection. Tuberculosis research was performed in cattle (n = 149) with TB-compatible lesions collected over 5 months from a slaughterh… Leer más

December 1st, 2017

Servicio de Informática y Comunicación

Preventing and reducing the transmission of antimicrobial resistance

The "HECTOR" project brings together research groups from Germany, the Netherlands, Spain and the United Kingdom 

Antibiotics have revolutionized human and veterinary medicine and saved countless lives. Their discovery represented a quantum leap in the treatment of infectious diseases. However, unfortunately, the global emergence and steady increase of bacteria resistant to multiple antimicrobials have jeopardized their efficacy, to the extent that resistant strains have become a major public health threat. Moreover, there is rising concern that some bacterial infections may become untreatable in the future. Multiple factors have contributed to the present situation. One such factor, exemplified by the recent emergence and global spread of colistin resistance or of extended spectrum β-lactamases (ESBL), is the presence of resistance determinants on mobile genetic elements, such as plasmids and transposons, which are rapidly transmitted between the same and different bacterial species.… Leer más

December 1st, 2017

Vía publiDB

Emerging Infectious Diseases

Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain

Investigation published in Emerging Infectious Diseases

Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited… Leer más

December 1st, 2017

Vía publiDB

Journal of Clinical Microbiology

Multi-Laboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens

Journal of Clinical Microbiology publish this investigation article

A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M. caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotyping or qPCR) in each laboratory; liquid (MGIT or BacT/Alert) and/or solid (Stonebrink, Coletsos or Lowenstein-Jensen) cultures were tested. In comparison to spoligotyping of acid-fast positive MGIT cultures, percentage agreement between positive LFD and spoligotyping results was ex… Leer más

December 1st, 2017

Vía publiDB

Research in Veterinary Science

Differentiation of Flavobacterium psychrophilum from Flavobacterium psychrophilum-like species by MALDI-TOF mass spectrometry

Investigation published in Research in Veterinary Science

Rainbow trout fry syndrome (RTFS) is an important infectious disease caused by Flavobacterium psychrophilum affecting farmed salmonids worldwide. Other Flavobacterium psychrophilum-like species (F. plurextorum, F. oncorhynchi, F. tructae, F. collinsii and F. piscis) have been isolated from diseased rainbow trout fry suspected of RTFS although the epidemiological and clinical relevance of these pathogens are unknown. The objective of this study was to evaluate the potential use of MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) Mass Spectrometry as method for specific ide… Leer más

November 15th, 2017

Vía publiDB

Frontiers in Microbiology

Genome comparison of erythromycin resistant Campylobacter from turkey identifies hosts and pathways for horizontal spread of erm(B) genes

Investigation article published in Frontiers in Microbiology

Pathogens in the genus Campylobacter are the most common cause of food-borne bacterial gastro-enteritis. Campylobacteriosis, caused principally by Campylobacter jejuni and C. coli, is transmitted to humans by food of animal origin, especially poultry. As for many pathogens, antimicrobial resistance in Campylobacter is increasing at an alarming rate. Erythromycin prescription is the treatment of choice for clinical cases requiring antimicrobial therapy but this is compromised by mobility of the erythromycin resistance gene erm(B) between strains. Here we evaluate resistance to six antimicrobial… Leer más

November 10th, 2017

Vía publiDB

Journal of Fish Diseases

First analysis by MALDI-TOF MS technique of Chryseobacterium species relevant

Journal of Fish Diseases publish this investigation article

November 6th, 2017

Vía publiDB

Diego Flórez Cuadrado

Genomic characterization and antimicrobial resistance of Campylobacter

PhD Thesis defense by Diego Flórez Cuadrado at the Faculty of Veterinary Medicine of de Complutense University of Madrid

Bacteria of the genus Campylobacter, mainly C. jejuni and C. coli, represent the most frequent cause of gastroenteritis of alimentary origin worldwide. These microorganisms colonize the digestive tract of birds and mammals. The main route of infection for humans is through the consumption of contaminated poultry meat. The infection produced by Campylobacter is usually self-limiting but can occasionally produce complications that require antimicrobial treatment. Erythromycin is the main treatment of choice. Resistance to erythromycin in Campylobacter has been related to mutations in genes or… Leer más

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Scientific publications
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Last Research lines
Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ICM 186 ZTA 76 SUAT 14
2 ZTA 179 SUAT 74 MYC 13
3 MYC 159 MYC 64 ZTA 11
4 SUAT 146 ICM 42 ICM 11
5 NED 65 NED 13 NED 2
  VISAVET 731 279 54

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.45 ZTA 3.519 ZTA 3.769
2 MYC 2.6 ICM 2.488 ICM 2.75
3 SUAT 2.449 SUAT 2.46 SUAT 2.489
4 ICM 2.285 SEVISEQ 2.251 SEVISEQ 2.442
5 SEVISEQ 2.251 SAP 2.236 MYC 2.276
VISAVET 2.617 2.643 2.762

ISI Scientific Publications
Total Last 60 mo.
1 Vet. Microbiol. 59 Transbound. Emerg. Dis. 24
2 J. Clin. Microbiol. 38 Vet. Microbiol. 22
3 Transbound. Emerg. Dis. 35 Res. Vet. Sci. 21
4 Int. J. Syst. Evol. Microbiol. 31 PLoS ONE 17
5 Res. Vet. Sci. 30 BMC Vet. Res 15
  All journals 207   All journals 34

PhD Thesis
Total Last 60 mo. Last 12 mo.
1 ZTA 20 ZTA 9 ZTA 4
2 SUAT 20 SUAT 8 NED 1
3 ICM 11 ICM 3 SUAT 1
4 MYC 10 MYC 2
5 NED 5 NED 2
  VISAVET 71 25 6

Master Thesis
Total Last 60 mo. Last 12 mo.
1 SUAT 17 ZTA 5 ZTA 1
2 ZTA 13 MYC 3
3 MYC 9 NED 2
4 ICM 7 SAP 1
5 NED 6 ICM 1
  VISAVET 54 12 1