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Last research news
September 1st, 2018
Vía publiDB
Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii in the Intensive Care Unit of Uruguay`s University Hospital Identifies the First rmtC Gene in the Species
Microbial Drug Resistance (Larchmont, N.Y.) publish this investigation article
Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are an increasing concern in intensive care units (ICUs) worldwide. The combination of carbapenemases and 16S rRNA-methyltransferases (16S-RMTases) further reduces the therapeutic options. OXA-carbapenemase/A. baumannii clone tandems in Latin America have already been described; however, no information exists in this region regarding the occurrence of 16S-RMTases in this microorganism. In addition, the epidemiology of A. baumannii in ICUs and its associated resistance profiles are poorly understood. Our objectives were as follows: …
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September 1st, 2018
Vía publiDB
Novel and highly sensitive sybr® green real-time pcr for poxvirus detection in odontocete cetaceans
Investigation published in Journal of Virological Methods
Poxviruses are emerging pathogens in cetaceans, temporarily named `Cetaceanpoxvirus` (CePV, family Poxviridae), classified into two main lineages: CePV-1 in odontocetes and CePV-2 in mysticetes. Only a few studies performed the molecular detection of CePVs, based on DNA-polymerase gene and/or DNA-topoisomerase I gene amplification. Herein we describe a new real-time PCR assay based on SYBR® Green and a new primer set to detect a 150 bp fragment of CePV DNA-polymerase gene, also effective for conventional PCR detection. The novel real-time PCR was able to detect 5 up to 5 × 106 copi…
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August 29th, 2018
Vía publiDB
MultiOrgan Disease and Death Associated With Streptococcus equi spp. zooepidemicus in a 2-Month-Old Foal
Investigation article published in Journal of Equine Veterinary Science
A 2-month-old Andalusian colt in central Spain presented dyspnea, diarrhea, and depression. Despite the initial treatment for presumptive pneumonia caused by Rhodococcus equi, after 1 hour, the colt showed signs of shock and died. The necropsy revealed multiple abscesses in the lung, as well as enlarged mesenteric lymph nodes, and hemorrhagic peritonitis. Samples were taken from trachea, lung parenchyma, small intestine, and colon. All samples yielded a pure culture of Streptococcus equi spp. zooepidemicus, whereas R. equi was excluded by using a specific qPCR performed on all samples. Isolate…
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August 25th, 2018
Vía publiDB
Modeling cost-effectiveness of risk-based bovine tuberculosis surveillance in Minnesota
Investigation published in Preventive Veterinary Medicine
In the United States, slaughter surveillance combined with other measures has effectively maintained a very low prevalence of bovine tuberculosis (bTB). However, bTB continues to be sporadically detected, causing substantial economic burden to the government and cattle producers. To detect the infection earlier and reduce sudden economic losses, additional risk-based surveillance of live animals might be more cost-effective than slaughter surveillance alone to detect and prevent bTB infection. The objective of this study was to evaluate alternative risk-based surveillance strategies targeting …
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August 24th, 2018
Vía publiDB
Genetic heterogeneity of dolphin morbilliviruses detected in the Spanish Mediterranean in inter-epizootic period
Investigation article published in BMC Veterinary Research
BACKGROUND:
In the last 20 years, Cetacean Morbillivirus (CeMV) has been responsible for many die-offs in marine mammals worldwide, as clearly exemplified by the three dolphin morbillivirus (DMV) epizootics of 1990-1992, 2006-2008 and 2011 that affected Mediterranean striped dolphins (Stenella coeruleoalba). Systemic infection caused by DMV in the Mediterranean has been reported only during these outbreaks.
RESULTS:
We report the infection of five striped dolphins (Stenella coeruleoalba) stranded on the Spanish Mediterranean coast of Valencia after the last DMV outbreak that ended in 2011…
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August 15th, 2018
Vía publiDB
Could African swine fever and classical swine fever viruses enter into the United States via swine products carried in air passengers` luggage?
Transboundary and Emerging Diseases publish this investigation article
On average 8,000 pork derived products are annually confiscated by Customs and Border Protection at the United States (US) ports of entry such as international airports, harbours or mail offices. These swine products with unknown sanitary status could pose a risk for foreign animal diseases introduction into the US. This study aimed at analysing the risk of African swine fever virus (ASFV) and classical swine fever virus (CSFV) being introduced into the US through prohibited swine products carried by air passengers (PSPAP) and identifying locations and time periods at higher risk where and whe…
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August 13rd, 2018
Vía publiDB
Development and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas
Investigation article published in Frontiers in veterinary science
South American camelids are susceptible to tuberculosis, caused mainly by Mycobacterium bovis and M. microti. Despite the tuberculin skin test being the official test for tuberculosis, it has a very low sensitivity in these species (14–20%). Serological tests present the advantages of being rapid, easy to perform and facilitate analysis of large numbers of samples in a short period of time. Novel antigen discovery and evaluation would provide enhanced detection of specific antibodies against members of M. tuberculosis complex. Here, we describe the development and evaluation of an ELISA-type i…
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August 1st, 2018
Vía publiDB
Exploring the oxidative, antimicrobial and genomic properties of Campylobacter jejuni strains isolated from poultry
Investigation article published in Research in Veterinary Science
Campylobacter jejuni is the leading cause of food-borne bacterial enteritis in humans, with contaminated poultry products considered the main source of infection. To survive the food chain, C. jejuni utilizes multiple defense mechanisms that counter oxidative and aerobic stresses. In this study, we phenotypically characterised 63 C. jejuni strains with oxidative stress survival and antimicrobial susceptibility testing to investigate correlations between these two phenotypes against the source of the strains and the presence of the MarR regulators RrpA and RrpB which have a role in regulating t…
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July 27th, 2018
Servicio de Informática y Comunicación
José Antonio Escudero, nuevo ERC 2018
Nuestro investigador ha sido seleccionado para la financiación en el campo de las ciencias de la vida por su proyecto KryptonInt
El proyecto "Erasing the superintegron to understand the role of chromosomal integrons in bacterial evolution"
(KryptonInt) dirigido por José Antonio Escudero, investigador de la Universidad Complutense de Madrid, ha sido seleccionado para las financiaciones Starting Grants 2018 del European Research Council (ERC).…
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July 27th, 2018
Vía publiDB
Different lesion distribution in calves orally or intratracheally challenged with Mycobacterium bovis: implications for diagnosis
Investigation published in Veterinary Research
Animal tuberculosis (TB) remains a major problem in some countries despite the existence of control programmes focused mainly on cattle. In this species, aerogenous transmission is accepted as the most frequent infection route, affecting mainly the respiratory system. Under the hypothesis that the oral route could be playing a more relevant role in transmission, diagnosis and disease persistence than previously thought, this study was performed to assess the course of TB infection in cattle and its effects on diagnosis depending on the route of entry of Mycobacterium bovis. Two groups of five …
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