Research

Asociación entre resistencia a antibióticos y factores del hospedador y la bacteria en patógenos de transmisión alimentaria

Degree Final Project defended by María Juez García

Salmonellosis is one of the main foodborne zoonosis worldwide and also in the European Union. It is caused by Salmonella enterica subesp. enterica, that can be divided in over 2,500 serotypes. Salmonella is a very ubiquitous bacteria that is also very resistant to a wide range of environmental conditions. Its importance is further complicated by the increasing problem of antimicrobial resistance. Among the large variety of food products associated with its transmission, egg and egg products are among the most frequently reported sources of infection. Salmonellosis mainly leads to gastrointesti… Leer más

Estudio sobre el efecto de los corticoides y la presensibilización con tuberculina empleados con fine fraudulentos en la prueba de intradermotuberculinización (IDTB)

Abel Díaz Castillo defended this Degree Final Project

Detección de Bornavirus tipo (BDV-1) mediante RT-PCR a tiempo real

Degree Final Project defended by Lucía Aroca Lara

Carbapenemase-producing Elizabethkingia meningoseptica from healthy pigs associated with colistin use in Spain

Investigation published in Antibiotics-Basel

Carbapenems are considered last-resort antimicrobials, especially for treating infections involving multidrug-resistant Gram-negative bacteria. In recent years, extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Gram-negative bacteria have become widespread in hospitals, community settings, and the environment, reducing the range of effective therapeutic alternatives. The use of colistin to treat infection caused by these multi-drug bacteria may favour the selection and persistence of carbapenem-resistant bacteria. In this study, it is described, for the first time to our kn… Leer más

Modeling the Accuracy of Two in-vitro Bovine Tuberculosis Tests Using a Bayesian Approach

Frontiers in veterinary science publish this investigation article

Accuracy of new or alternative diagnostic tests is typically estimated in relation to a well-standardized reference test referred to as a gold standard. However, for bovine tuberculosis (bTB), a chronic disease of cattle, affecting animal and public health, no reliable gold standard is available. In this context, latent-class models implemented using a Bayesian approach can help to assess the accuracy of diagnostic tests incorporating previous knowledge on test performance and disease prevalence. In Uruguay, bTB-prevalence has increased in the past decades partially because of the limited accu… Leer más

The bla NDM-1 carrying IncA/C2 plasmid underlies structural alterations and co-integrate formation in vivo

Antimicrobial Agents and Chemotherapy publish this investigation article

In 2012, a carbapenemase-producing Salmonella (S.) Corvallis carrying a bla NDM-1 multi-resistance IncA/C2 plasmid, apart from IncHI2 and ColE-like plasmids, was detected in a wild bird in Germany. In recent broiler chicken infection study, we observed transfer of this bla NDM-1-carrying IncA/C2 plasmid to other Enterobacteriaceae Here, we focused on the stability of this plasmid and gained insight into type and frequency of its structural alterations after an in vivo passage in a broiler infection study… Leer más

Identificación de factores predictores de virulencia en cepas de Mycobacterium bovis

Alberto Gómez Buendía defended this Degree Final Project

Detección molecular de Trypanosoma cruzi en pelo de ratón infectado experimentalmente

Degree Final Project defended by Silvia Díaz Franchy

Trypanosoma cruzi es el parásito protozoo causante de la enfermedad de Chagas, que afecta entre 6 y 8 millones de personas. Es una enfermedad transmitida principalmente por insectos de la familia Reduviidae, aunque también se puede transmitir por vía oral, mediante transfusiones o vía congénita. Se trata de una enfermedad infradiagnosticada en Europa, siendo España el país europeo con mayor número de personas afectadas por T. cruzi. En los países endémicos los animales salvajes suponen uno de los principales reservorios, haciendo el manejo y obtención de muestras difícil. Por tanto, se ha cons… Leer más

A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)

Investigation article published in Transboundary and Emerging Diseases

In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT), and quantitative real‐time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DF… Leer más

Desarrollo y puesta a punto de una técnica de base inmunológica para la detección conjunta de ricina y aglutinina

Presentation by Virginia Colmena García to obtain the Master in Disaster Management

La ricina es una de las toxinas más potentes conocidas, que se encuentra en la semilla del ricino (Ricinus communis). El Centro para el Control y la Prevención de Enfermedades de Estados Unidos (CDC) la clasifica como posible arma biológica. Existen diferentes métodos para la detección de esta proteína, siendo la técnica inmunológica ELISA, especialmente el ELISA tipo sándwich, la que está considerada como la técnica más robusta para su análisis, ya que es sensible, específica, con límites de detección de picogramos y fácil de realizar. No obstante, el reto está en poder diferenciar la ricina … Leer más