Research

Prevención y tratamiento de diarreas postdestete en lechones. Cuantificación de E. coli positivos a mcr-1 mediante real time-qPCR con SYBR Green I en heces de porcino

Cristina Montes Solís defended this presentation at the Faculty of Veterinary Medicine of the Complutense University of Madrid in order to obtain the Master of Research

Experts, ranchers and hunting managers work on tuberculosis control

The supra-regional operating group "Tuberculosis" has held a new working meeting

The members of the supra-regional Operative Group "Tuberculosis" have held a meeting in Madrid to continue working on the development of an innovation and transfer project whose objective is to implement management measures that contribute to the control of a disease as important as bovine tuberculosis.… Leer más

Motion-based video monitoring for early detection of livestock diseases: The case of African swine fever

Investigation published in PLoS ONE

Early detection of infectious diseases can substantially reduce the health and economic impacts on livestock production. Here we describe a system for monitoring animal activity based on video and data processing techniques, in order to detect slowdown and weakening due to infection with African swine fever (ASF), one of the most significant threats to the pig industry. The system classifies and quantifies motion-based animal behaviour and daily activity in video sequences, allowing automated and non-intrusive surveillance in real-time. The aim of this system is to evaluate significant changes… Leer más

Short communication: Isolation frequency of bacteria causing lymphadenitis and abscesses in small ruminants in central Spain

Small Ruminant Research publish this investigation article

Infectious lymphadenitis in small ruminants caused by Corynebacterium pseudotuberculosis and Staphylococcus aureus subsp. anaerobius are widely distributed throughout the world, and result in significant economic losses. Trueperella pyogenes has also been associated with lymphadenitis in sheep and goats. In order to determinate the isolation frequency of the different agents associated with lymphadenitis and abscesses, 171 pus samples (135 from sheep and 36 from goats) from 46 flocks were investigated. Isolated bacteria were identified by MALDI-TOF method. S. aureus subsp. anaerobius was the m… Leer más

The mobile dso-gene-sso element in rolling circles plasmids of staphylococci reflects the evolutionary history of its resistance gene

Investigation article published in Letters in Applied Microbiology

The qacC and lnuA genes of Staphylococcus species were recently proposed to comprise a mobile element when residing on rolling-circle plasmids. Here we present other examples of resistance genes on staphylococcal rolling-circle plasmids, including fosB producing resistance to fosfomycin, cat resulting in resistance to chloramphenicol, and cadB for resistance to the toxic heavy metal cadmium. For three of these genes (qacC, lnuA and fosB) evidence was obtained that the genes have spread between different plasmid backgrounds. The lack of mutations in qacC suggests that the spread occurred relati… Leer más

Application of a specific quantitative real-time PCR (qPCR) to identify Leishmania infantum DNA in spleen, skin and hair samples of wild Leporidae

Veterinary Parasitology publish this investigation article

The aim of this study was to compare a quantitative real-time PCR (qPCR) validated for the detection of Leishmania infantum in dogs with a nested PCR but in wild Leporidae. Additionally, L. infantum results from indirect immunofluorescent antibody test (IFAT) and in vitro culture were also compared with qPCR. Different samples (spleen, skin and hair) recovered from 224 European rabbits and 70 Iberian hares from two green areas of Madrid Council were analysed for the detection of L. infantum. The presence of Leishmania kDNA was detected by qPCR in 58 out of 221 (26.24%), 162 out of 203 (79.8%) … Leer más

The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain

Investigation article published in Veterinary Microbiology

Deer species (family Cervidae) are often part of the Mycobacterium tuberculosis complex maintenance host community, and tuberculosis (TB) control in deer, including vaccination, is consequently an area of ongoing research. However, most research into deer vaccination against TB is focused on using the live bacillus Calmette Guerin (BCG). Oral inactivated vaccines represent an interesting alternative to either oral or parenteral BCG, since neither diagnostic cross-reactions nor vaccine strain survival are likely to occur. In order to describe the red deer response to heat-inactivated M. bovis (… Leer más

Oral Vaccination with Heat-Inactivated Mycobacterium bovis Does Not Interfere with the Antemortem Diagnostic Techniques for Tuberculosis in Goats

Frontiers in veterinary science publish this investigation article

Vaccination against tuberculosis (TB) is prohibited in cattle or other species subjected to specific TB eradication campaigns, due to the interference that it may cause with the official diagnostic tests. However, immunization with a heat-inactivated (HI) Mycobacterium bovis vaccine via the oral route has been suggested to overcome this issue. In this study, the main goal was to assess the interference of the HI vaccine by different routes of administration using a previous vaccination and re-vaccination (boosting) protocol. TB-free kid goats were divided into three groups: oral (n = 16), intr… Leer más

Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015

Investigation published in Eurosurveillance

Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.… Leer más

Escherichia coli ST167 carrying plasmid mobilisable mcr-1 and blaCTX-M-15 resistance determinants isolated from a human respiratory infection

Investigation published in International Journal of Antimicrobial Agents

The emergence of polymyxin resistance in Enterobacteriaceae is a matter of major concern and has instigated the recommendation for its surveillance since it compromises the use of colistin, a last-resort antibiotic.… Leer más