Last SCI scientific publications
Trans-species transmission of Brucellae among ruminants hampering brucellosis control efforts in Egypt
Journal Of Applied Microbiology 132(1):90-100.
This study aimed to identify the genotypic fingerprinting of B. melitensis biovar 3 isolates from ruminants in Kafr El-Sheikh, Egypt, to compare with other peers globally, and to highlight the epidemiology and potential causes of brucellosis control failure.
A multilocus variable-number tandem-repeat analysis (MLVA 16) was carried out on 41 B. melitensis bv3 isolates, 31 from the preferential hosts (28 sheep and three goats), and ten from atypical hosts (nine cattle and one buffalo), identified by bacteriological and molecular techniques. MLVA-16 analysis revealed 19 genotypes with nine as singletons. The most prevalent genotypes were M3_K.E (3,5,3,13,1,1,3,3,7,43,8,7,6,7,5,3), M13_K.E (3,5,3,13,1,1,3,3,7,43,8,5,8,7,7,3), and… Leer más
Hegazy Y., Abdel-Hamid N., Eldahiey M., Oreiby A., Algaabary M., Hamdy M., Beleta E., Martinez I., Shahein M., Garcia N. and Eltholth M.
Genomic and Pathogenic Investigations of Streptococcus suis Serotype 7 Population derived from a Human Patient and Pigs
Emerging Microbes And Infections 1-41.
Streptococcus suis is one of the important emerging zoonotic pathogens. Serotype 2 is most prevalent in patients worldwide. In the present study, we first isolated one S. suis serotype 7 strain GX69 from the blood culture of a patient with septicemia complicated with pneumonia in China. In order to deepen the understanding of S. suis serotype 7 population characteristics, we investigated the phylogenetic structure, genomic features, and virulence of S. suis serotype 7 population, including 35 strains and 79 genomes. Significant diversities were revealed in S. suis serotype 7 population, which were clustered into 22 sequence types (STs), five minimum core genome (MCG) groups, and six lineages. Lineages 1, 3a, and 6 were mainly constituted by… Leer más
Liang P., Wang M., Gottschalk M., Vela AI., Estrada AA., Wang J., Du P., Luo M., Wu Z. and Zheng H.
Safety of African Swine Fever Vaccine Candidate Lv17/WB/Rie1 in Wild Boar: Overdose and Repeated Doses
Frontiers In Immunology 12:761753.
African swine fever (ASF) is a highly lethal infectious disease that affects domestic pigs and wild boar. Outbreaks of ASF have grown considerably in the last decade causing important economic consequences for the swine industry. Its control is hampered by the lack of an effective treatment or vaccine. In Europe, the wild boar is a key wild reservoir for ASF. The results of the oral vaccination trial of wild boar with Lv17/WB/Rie1 are hope for this problem. However, this vaccine candidate has certain safety concerns, since it is a naturally attenuated vaccine. Therefore, the current study aims to evaluate the safety of this vaccine candidate in terms of overdose (high dose) and repeated doses (revaccination) in wild boar. Low-dose orally va… Leer más
Barasona JA., Cadenas-Fernández E., Kosowska A., Barroso-Arevalo S., Rivera B., Sanchez R., Porras-Gonzalez N., Gallardo C. and Sanchez-Vizcaino JM..
Evolution of ColE1-like plasmids across γ-Proteobacteria: From bacteriocin production to antimicrobial resistance
PLoS Genetics 17(11):e1009919.
Antimicrobial resistance is one of the major threats to Public Health worldwide. Understanding the transfer and maintenance of antimicrobial resistance genes mediated by mobile genetic elements is thus urgent. In this work, we focus on the ColE1-like plasmid family, whose distinctive replication and multicopy nature has given rise to key discoveries and tools in molecular biology. Despite being massively used, the hosts, functions, and evolutionary history of these plasmids remain poorly known. Here, we built specific Hidden Markov Model(HMM) profiles to search ColE1 replicons within genomes. We identified 1,035 ColE1 plasmids in five Orders of γ-Proteobacteria, several of which are described here for the first time. The phylogenetic a… Leer más
Ares M., Rocha EPC. and Gonzalez-Zorn B..
Differences in wild boar spatial behaviour among land uses and management scenarios in Mediterranean ecosystems
Science Of The Total Environment 796:148966.
The ubiquitous wild boar is causing diverse and growing conflicts of socio-ecological and economic relevance worldwide. For that reason, knowledge of its spatial ecology is crucial to designing effective management programmes. But this knowledge is scarce in Mediterranean areas with mixed land uses. We describe the spatial ecology and habitat selection of 41 adult wild boar monitored using GPS collars and analyse the effects of sex and the period (food shortage period, hunting season and food abundance period) under different land uses (protected areas, mixed farms and fenced hunting estates). The spatial ecology of wild boar was characterised by marked temporality, mediated by sex and the land uses in the area. The activity (ACT), daily ra… Leer más
Laguna E., Barasona JA., Vicente J., Keuling O. and Acevedo O.
Bottlenose dolphins (Tursiops truncatus) aggressive behavior towards other cetacean species in the western Mediterranean
Scientific Reports 11(1):21582.
Aggressive behavior of bottlenose dolphins (Tursiops truncatus) towards conspecifics is widely described, but they have also often been reported attacking and killing harbour porpoises (Phocoena phocoena) around the world. However, very few reports exist of aggressive interactions between bottlenose dolphins and other cetacean species. Here, we provide the first evidence that bottlenose dolphins in the western Mediterranean exhibit aggressive behavior towards both striped dolphins (Stenella coeruleoalba) and Risso`s dolphins (Grampus griseus). Necropsies and visual examination of stranded striped (14) and Risso`s (2) dolphins showed numerous lesions (external rake marks and different bone fractures or internal organ damage by blunt trauma).… Leer más
Crespo JL., Rubio-Guerri C., Jimenez MA., Aznar JF., Marco-Cabedo V., Melero M., Sanchez-Vizcaino JM., Gozalbes P. and Garcia-Parraga D.
A new method for sampling African swine fever virus genome and its inactivation in environmental samples
Scientific Reports 11(1):21560.
African swine fever (ASF) is currently the most dangerous disease for the global pig industry, causing huge economic losses, due to the lack of effective vaccine or treatment. Only the early detection of ASF virus (ASFV) and proper biosecurity measures are effective to reduce the viral expansion. One of the most widely recognized risks as regards the introduction ASFV into a country is infected animals and contaminated livestock vehicles. In order to improve ASF surveillance, we have assessed the capacity for the detection and inactivation of ASFV genome by using Dry-Sponges (3 M) pre-hydrated with a new surfactant liquid. We sampled different surfaces in ASFV-contaminated facilities, including animal skins, and the results were compared to… Leer más
Kosowska A., Barasona JA., Barroso-Arevalo S., Rivera B., Dominguez L. and Sanchez-Vizcaino JM..
Large-scale study on virological and serological prevalence of SARS-CoV-2 in cats and dogs in Spain
Transboundary And Emerging Diseases 1-16.
The disease produced by the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), is currently one of the primary concerns worldwide. Knowing the zoonotic origin of the disease and that several animal species, including dogs and cats, are susceptible to viral infection, it is critical to assess the relevance of pets in this pandemic. Here, we performed a large-scale study on SARS-CoV-2 serological and viral prevalence in cats and dogs in Spain in order to elucidate their role and susceptibility. Samples from animals in contact with COVID-19 positive people and/or compatible symptoms (n = 492), as well as from random animals (n = 1024), were taken. Despite the large number of animals analyzed, only 12 animals (eight dogs and … Leer más
Barroso-Arevalo S., Barneto A., Ramos AM., Rivera B., Sanchez R., Sanchez-Morales I., Perez-Sancho M., Buendia A., Ferreras E., Ortiz-Menendez JC., Moreno I., Serres C., Vela C., Risalde MA., Dominguez L. and Sanchez-Vizcaino JM..
Equine infection with Leishmania spp. in Costa Rica: Study of five cases
Veterinary Medicine And Science 7(6):2234-2239.
Cutaneous forms of leishmaniosis due to Leishmania braziliensis have been reported in horses in the New World. Domestic animals play a role in the transmission of the disease. In Costa Rica, human cases of L. braziliensis, L. panamensis and L. infantum have been reported. The present report describes five cases of equine cutaneous leishmaniosis in Costa Rica. The aetiological diagnosis was based on the presence of the parasite within the lesions. Skin biopsies were used to perform histopathological analyses of the lesions. Immunohistochemistry was used to detect the presence of the Leishmania spp. antigens in tissue sections. Laser-capture micro-dissection and quantitative real-time PCR techniques were carried out to detect the pathogen nuc… Leer más
Ortega-García MV., Salguero FJ., Garcia N., Dominguez M., Moreno I. and Berrocal A.
Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions
Viruses-Basel 13(11).
The monitoring of herpesvirus infection provides useful information when assessing marine mammals’ health. This paper shows the prevalence of herpesvirus infection (80.85%) in 47 cetaceans stranded on the coast of the Valencian Community, Spain. Of the 966 tissues evaluated, 121 tested positive when employing nested-PCR (12.53%). The largest proportion of herpesvirus-positive tissue samples was in the reproductive system, nervous system, and tegument. Herpesvirus was more prevalent in females, juveniles, and calves. More than half the DNA PCR positive tissues contained herpesvirus RNA, indicating the presence of actively replicating virus. This RNA was most frequently found in neonates. Fourteen unique sequences were identified. Most amplif… Leer más
Vargas-Castro I., Melero M., Crespo JL., Jimenez MA., Sierra E., Rubio-Guerri C., Arbelo M., Fernandez A., Garcia-Parraga D. and Sanchez-Vizcaino JM..
