Last SCI scientific publications
Identifying high-risk areas for dog-mediated rabies using Bayesian spatial regression
One Health 15:100411.
Despite ongoing control efforts, rabies remains an endemic zoonotic disease in many countries. Determining high-risk areas and the space-time patterns of rabies spread, as it relates to epidemiologically important factors, can support policymakers and program managers alike to develop evidence-based targeted surveillance and control programs. In this One Health approach which selected Thailand as the example site, the location-based risk of contracting dog-mediated rabies by both human and animal populations was quantified using a Bayesian spatial regression model. Specifically, a conditional autoregressive (CAR) Bayesian zero-inflated Poisson (ZIP) regression was fitted to the reported human and animal rabies case counts of each district, … Leer más
Kanankege K., Errecaborde KM., Wiratsudakul A., Wongnak P., Yoopatthanawong C., Thanapongtharm W., Alvarez J. and Perez A.
Environment and Offspring Surveillance in Porcine Brucellosis
Frontiers In Veterinary Science 1-7.
Porcine brucellosis, caused by Brucella suis (B. suis), is a notifiable disease causing significant economic losses in production systems. Most infected pigs may act as carriers and shed B. suis even if asymptomatic. This can contribute to environmental persistence, thus hindering control efforts. Here, the environment and the offspring were investigated during and after a B. suis outbreak at a sow breeding farm. The diagnosis of B. suis in sows (n = 1,140) was performed by culture and polymerase chain reaction (PCR) from vaginal swabs, indirect enzyme-linked immunosorbent assay (I-ELISA) from sera, and brucellin skin test (BST). B. suis diagnosis in post-weaning pigs (n = 899) was performed by I-ELISA in sera and BST. The environmental sur… Leer más
Rebollada A., Perez-Sancho M., Rodriguez-Bertos A., Garcia N., Martinez I., Navarro A., Dominguez L. and Garcia-Seco T..
Bacteremia and Aortic Valvular Endocarditis in a Eurasian Stone-Curlew (Burhinus oedicnemus distinctus) Due to Streptococcus dysgalactiae
Journal Of Wildlife Diseases 1.
Burhinus oedicnemus distinctus is an endemic subspecies of Eurasian Stone-curlew present in the Canary Islands. Their populations are rapidly declining, mainly because of anthropogenic impacts. This report describes valvular endocarditis and septicemia in a Eurasian Stone-Curlew with left foot loss and severe contralateral bumblefoot… Leer más
Suarez-Santana C., Fernandez A., Quesada-Canales O., Vela AI., Sarmiento-Navarro J. and Sierra E.
Feline Gastrointestinal Eosinophilic Sclerosing Fibroplasia-Extracellular Matrix Proteins and TGF-β1 Immunoexpression
Veterinary Sciences 9(6):291.
Feline gastrointestinal eosinophilic sclerosing fibroplasia (FGESF) has been described as an inflammatory disorder with an eosinophilic component with etiopathogenesis that is still unknown. Sixteen intestinal samples from two veterinary diagnostic services (2014–2017) were included in the study. A histopathological criterion classified the cases into three grades (mild, moderate, and severe) according to the distribution of the lesions and the course. An immunohistochemical study of collagen I, collagen III, fibronectin, and transforming growth factor β1 (TGF-β1) was performed in each case. An immunohistochemical study of mild grades shows greater collagen III immunoexpression, compared to collagen I and fibronectin, which sugges… Leer más
Porras-Gonzalez N., Rebollada A., Rodriguez-Franco F., Calvo-Ibbitson A. and Rodriguez-Bertos A..
Molecular detection of Tritrichomonas foetus in bovine samples: a novel real-time polymerase chain reaction (PCR) assay targeting EF1-alpha-Tf1 and a comparative study of published PCR techniques
Parasitology Research 00.
The parasite T. foetus causes trichomonosis in cattle but is generally asymptomatic in males. Thus, many bulls carrying the disease go unnoticed, making the detection of T. foetus in bulls an important aspect for its control. Due to drawbacks posed by its cultivation, PCR is a preferred option for diagnostic laboratories. Most published PCR protocols target the genomic region compring the 18S, 5.8S, and 28S rRNA genes and internal transcribed spacers 1 and 2 (rRNA-ITS region), homologous to that of other Tritrichomonas species. There is minimal information on alternative genetic targets and no comparative studies have been published. We compared a protocol based on the microsatellite TfRE (called H94) and five protocols based on the rRNA-IT… Leer más
Polo C., Garcia-Seco T., Fernandez V., Hernandez M., Briones V., Diez-Guerrier A., Dominguez L. and Perez-Sancho M..
Permeability of artificial barriers (fences) for wild boar Sus scrofa in Mediterranean mixed landscapes
Pest Management Science 78(6):2277-2286.
Background: Fences are one of the most widespread manmade features in nature, constituting an artificial limitation to the movement of wildlife. To date, their effects on wildlife behaviour have been understudied but this knowledge is required to design effective management procedures. Using 21 GPS-monitored wild boar, we evaluated the permeability of different types of fences and described temporal patterns and spatial hotspots for crossing events. A fence`s permeability was inferred by the crossing success, i.e., the number of times that animals crossed a barrier vs the number of times they did not cross. The vulnerability of fences at watercourses was explored by assessing whether the frequency of crossings was higher around watercourse … Leer más
Laguna E., Barasona JA., Carpio AJ., Vicente J. and Acevedo P.
Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples
Research In Veterinary Science 145:40-45.
Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB eradication programmes are based mainly on intradermal tuberculin tests and slaughterhouse surveillance. However, the use of serological test has been extended as a potential diagnostic tool in goats through the use of serum, plasma, or even milk samples. Milk production and the antibodies (Ab) present in milk can vary depending on several circumstances. In the present study, different factors that may affect the performance of humoral TB diagnosis were analysed using goat milk samples: 1) lactation stage, 2) a recent previous skin test (booster effect) and 3) the effect of freeze-thaw cycles on milk samples preserved with … Leer más
Ortega J., Infantes-Lorenzo JA., Roy A., de Juan L., Romero B., Moreno I., Dominguez M., Dominguez L. and Bezos J..
A subunit vaccine candidate based on the Spike protein of SARS-CoV-2 prevents infectious virus shedding in cats
Research In Veterinary Science 1-36.
Of the numerous animal species affected by the SARS-CoV-2 virus, cats are one of the most susceptible, and cat-to-cat transmission has been described. Although cat-to-human infection has not, as yet, been demonstrated, preventive measures should be taken in order to avoid both viral infection in cats and transmission among them. In this respect, the application of an effective vaccine to at-risk populations would be a useful tool for controlling the disease in this species. Here, we test a new vaccine prototype based on the Spike protein of the virus in order to prevent infection and infectious virus shedding in cats. The vaccine employed in experimentation, and which is easily produced, triggered a strong neutralizing antibody response in … Leer más
Barroso-Arevalo S., Sánchez-Morales L., Dominguez M., Garcia-Seco T., Risalde MA., Garcia-Bocanegra I., Dominguez L. and Sanchez-Vizcaino JM..
Clonal and plasmid-mediated flow of ESBL/AmpC genes in Escherichia coli in a commercial laying hen farm
Veterinary Microbiology 270:109453.
Resistance to third- and fourth-generation cephalosporins in Escherichia coli is mainly due to extended-spectrum beta-lactamases (ESBL) and AmpC cephalosporinases, which have been increasingly reported, mainly in isolates from humans and poultry.
The aim of this study was to address the flow of antimicrobial resistance determinants in the full laying hen production cycle (four batches followed from day-old chicks to 83/84-week-old layers), using cephalosporin-resistant E. coli as a model and their characterization using whole genome sequencing (WGS).
Fifteen out of 22 samples analysed yielded growth on MacConkey agar with cefotaxime (1 mg/L). Of these, 141 isolates were identified as E. coli and 47 were characterized by WGS.
Aldea-Ramos I., Gibello A., Hernandez M., Leekitcharoenphon P., Bortolaia V. and Moreno MA.
Zoonotic Transmission of Diphtheria from Domestic Animal Reservoir, Spain
Emerging Infectious Diseases 28:6.
Toxigenic Corynebacterium ulcerans is as an emerging zoonotic agent of diphtheria. We describe the zoonotic transmission of diphtheria caused by toxigenic C. ulcerans from domestic animals in Spain, confirmed by core-genome multilocus sequence typing. Alongside an increasing number of recent publications, our findings highlight the public health threat posed by diphtheria reemergence.
Diphtheria has been increasing in relevance because of increasing individual travel and surges in mass relocation events of refugees, asylum seekers, and immigrants from countries where diphtheria remains endemic (1–3). These importation events, in combination with growing vaccine hesitancy in nonendemic countries, give diphtheria a high potential for r… Leer más
Hoefer A., Herrera-Leon S., Dominguez L., Ordovas-Gavin M., Romero B., Araujo-Piedra XB., Sobrino-Calzada C., Uria-Gonzalez MJ., Herrera-Leon L. and Case Study Investigation Group.
