Last SCI scientific publications
Serological technique for detecting tuberculosis prevalence in sheep in Atlantic Spain
Research In Veterinary Science 129:96-98.
Recent studies show that sheep could be considered to be a maintenance host for the causative agents of animal tuberculosis (TB). The performance of diagnostic tests is not well established, and new tests need to be developed for this species. In addition, information about TB prevalence in sheep is scarce. Our objectives were to evaluate a new P22 ELISA for detection of specific antibodies against Mycobacterium tuberculosis Complex (MTC), and to assess the seropositivity in 3998 sheep from herds sampled in TB hotspot areas of northern Atlantic Spain with a low TB prevalence in cattle. Results based on 80 sheep of known infection status suggest excellent sensitivity and specificity (100% and 98%, respectively) even in a M. avium susbsp. par… Leer más
Infantes-Lorenzo JA., Gortazar C., Dominguez L., Munoz-Mendoza M., Dominguez M. and Balseiro A.
Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar
Pathogens 9(4), 242.
The presence of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex (MTC) is a main concern in wildlife populations such as the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against the MTC are valuable for tuberculosis (TB) monitoring and control and particularly useful in suids. The development of accurate, efficient, and non-invasive new tools to detect exposure to MTC would be highly beneficial for improving disease surveillance. This study aimed to determine if antibodies against MTC could be detected in oral fluid (OF) samples by a new ELISA test (IgG detection) from naturally TB-infected wild boar. For this, individual, paired serum and OF samples were collected from 148 live wild boar in two… Leer más
Barasona JA., Barroso-Arevalo S., Rivera B., Gortazar C. and Sanchez-Vizcaino JM..
Liver Transudate, a Potential Alternative to Detect Anti-Hepatitis E Virus Antibodies in Pigs and Wild Boars (Sus scrofa)
Microorganisms 8(3):450.
In recent years, cases of hepatitis E virus (HEV) infection have increased in Europe in association with the consumption of contaminated food, mainly from pork products but also from wild boars. The animal’s serum is usually tested for the presence of anti-HEV antibodies and viral RNA but, in many cases such as during hunting, an adequate serum sample cannot be obtained. In the present study, liver transudate was evaluated as an alternative matrix to serum for HEV detection. A total of 125 sera and liver transudates were tested by enzyme-linked immunosorbent assay at different dilutions (1:2, 1:10, 1:20), while 58 samples of serum and liver transudate were checked for the presence of HEV RNA by RT-qPCR. Anti- HEV antibodies were detected by… Leer más
Navarro A., Barcena C., Pozo P., Diez-Guerrier A., Martinez I., Polo Vaquero C., Duque C., Rodriguez-Lazaro D., Goyache J. and Garcia N..
Towards the comparison of home range estimators obtained from contrasting tracking regimes: the wild boar as a case study
European Journal Of Wildlife Research 66:3200.
Traditional VHF radio-tracking is gradually being replaced by GPS tracking devices in spatial ecology studies, although both technologies continue to be used. Differences between tracking regimes (time and fix frequency) may lead to home range estimates that are not directly comparable. Our primary aim was to test the reliability of comparisons in home range estimates using different estimators (minimum convex polygon [MCP], fixed kernels [KDE] and dynamic Brownian Bridge Models [dBBMM]) and tracking regimes (see above) and to provide an empirical basis for linking VHS and GPS tracking data analysis, using the wild boar (Sus scrofa) as a study model. Data were obtained from 15 GPS-collared individuals from three areas in Mediterranean Spain… Leer más
Peris A., Closa F., Marco I., Acevedo P., Barasona JA. and Casas Diaz E.
Antimicrobial susceptibility of Trueperella pyogenes isolated from food-producing ruminants
Veterinary Microbiology 242:108593.
A total of 96 Trueperella pyogenes isolates, an opportunistic pathogen of food-producing ruminants, obtained from cattle (n = 34), sheep (n = 35) and goats (n = 27), and identified by Real Time PCR (qPCR), were analysed to determine the susceptibility to 12 antimicrobials commonly used in livestock, using a broth microdilution. The Minimal Inhibitory Concentration (MIC) distribution was unimodal for half of the antimicrobials tested with the exception of apramycin, gentamicin, streptomycin, oxytetracycline, tylosin, and erythromycin all of which showed bimodal MIC distributions. Low MIC90 values for penicillin, amoxicillin, ceftiofur, enrofloxacin, and gentamicin (<1 μg/ml) were obtained, sugge… Leer más
Galan-Relaño A., Gomez-Gascon L., Barrero-Dominguez B., Luque I., Jurado-Martos F., Vela AI., Sanz-Tejero C. and Tarradas C.
Importance of equine piroplasmosis antibody presence in Spanish horses prior to export
Ticks And Tick-Borne Diseases 11:101329.
Serological analysis of equine piroplasmosis (EP), caused by Theileria equi and Babesia caballi, is included in the export testing requirements for most of the countries worldwide, thus involving a high economic impact on equine industry of EP-endemic countries, such as Spain. A total of 3368 serum samples from healthy horses collected prior to export between 2015 and 2018 in Spain were tested for antibodies against T. equi and B. caballi by using a competitive inhibition enzyme-linked immunosorbent assay (cELISA). The overall seroprevalence results in Spain revealed that almost a quarter of the horses analysed (24.1%; 95% CI 22.6-25.5) could not be exported to countries free from EP. The implementation of prevention measures such as the us… Leer más
Camino E., Pozo P., Dorrego Rodríguez A., Carvajal KA., Buendia A., Gonzalez S., de Juan L., Dominguez L. and Cruz F..
Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
Pathogens 9(171):1-14.
African swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insufficient and an effective vaccine against ASFV needs to be urgently developed. We hypothesized that immunization with a cocktail of thirty-five rationally selected antigens would improve the protective efficacy of subunit vaccine prototypes given that the combination of fewer immunogenic antigens (between 2 and 22) has failed to elicit protective efficacy. To this end,
immunogenicity and efficacy of thirty-five adenovirus-vectored ASFV antigens… Leer más
Cadenas-Fernández E., Sanchez-Vizcaino JM., Kosowska A., Rivera B., Mayoral-Alegre F., Rodriguez-Bertos A., Yao J., Bray J., Lokhandwala S., Mwangi W. and Barasona JA..
Prevalence and trend analysis of antimicrobial resistance in clinical Escherichia coli isolates collected from diseased pigs in the USA between 2006 and 2016
Transboundary And Emerging Diseases 00.
Antimicrobial resistance (AMR) is an emerging threat to both human and animal health. Antimicrobial use and resistance in food animal production including swine has received increased scrutiny as a source of resistant foodborne pathogens. Continuous surveillance of AMR in bacterial isolates of swine origin can guide in conservation of antimicrobials used in both human and swine medicine. The objective of this study was to evaluate the prevalence and trends of the phenotypic AMR in Escherichia coli of swine origin isolated from clinical samples at the Minnesota Veterinary Diagnostic laboratory between 2006‐2016. The prevalence of resistance to ampicillin, tetracyclines and sulphadimethoxine remained greater than 50% throughout the peri… Leer más
Hayer SS., Rovira A., Olsen K., Johnson TJ., Vannucci F., Rendahl A., Perez A. and Alvarez J..
Disease-mediated piglet mortality prevents wild boar population growth in fenced overabundant settings
European Journal Of Wildlife Research 66:26.
Assessing Eurasian wild boar (Sus scrofa) mortality is a key for understanding population dynamics and adjusting hunting harvest and population management. We used radio-tagging and video-trapping to quantify piglet summer mortality in a managed (i.e. fenced and year-round fed) wild boar population from southern Spain. We used two independent tools to assess wild boar mortality, i.e. radio-tracking and video footage. A total of 32 wild boar piglets were captured at selective feeders acting as cage-traps, and all the piglets were equipped with ear-tag transmitters including a mortality sensor. Additionally, 20 camera traps were set up in video mode at wild boar feeders. Videos were visualized calculating the proportion of piglets seen per ad… Leer más
Barasona JA., Risalde MA., Ortiz JA., Gonzalez-Barrio D., Che Amat A., Perez-Sancho M., Vargas-Castillo L., Xeidakis A., Jurado-Tarifa E. and Gortazar C.
Dynamic Network of Interactions in the Wildlife-Livestock Interface in Mediterranean Spain: An Epidemiological Point of View
Pathogens 9:120(1-16).
The correct management of diseases that are transmitted between wildlife and livestock requires a reliable estimate of the pathogen transmission rate. The calculation of this parameter is a challenge for epidemiologists, since transmission can occur through multiple pathways. The social network analysis is a widely used tool in epidemiology due to its capacity to identify individuals and communities with relevant roles for pathogen transmission. In the present work, we studied the dynamic network of interactions in a complex epidemiological scenario using information from different methodologies. In 2015, nine red deer, seven fallow deer, six wild boar and nine cattle were simultaneously monitored using GPS-GSM-Proximity collars in Doñana N… Leer más
Triguero-Ocana R., Martinez-Lopez B., Vicente J., Barasona JA., Martinez-Guijosa J. and Acevedo P.
