Last SCI scientific publications
Carbapenemase-producing Elizabethkingia meningoseptica from healthy pigs associated with colistin use in Spain
Antibiotics-Basel 8,146.
Carbapenems are considered last-resort antimicrobials, especially for treating infections involving multidrug-resistant Gram-negative bacteria. In recent years, extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Gram-negative bacteria have become widespread in hospitals, community settings, and the environment, reducing the range of effective therapeutic alternatives. The use of colistin to treat infection caused by these multi-drug bacteria may favour the selection and persistence of carbapenem-resistant bacteria. In this study, it is described, for the first time to our knowledge, a carbapenemase-producing isolate of Elizabethkingia meningoseptica from healthy pigs in Spain. The isolate we report was recovered during a … Leer más
Miguela-Villoldo P., Hernandez M., Moreno MA., Rodriguez-Lazaro D., Quesada A., Dominguez L. and Ugarte-Ruiz M..
Modeling the Accuracy of Two in-vitro Bovine Tuberculosis Tests Using a Bayesian Approach
Frontiers In Veterinary Science 6:261.
Accuracy of new or alternative diagnostic tests is typically estimated in relation to a well-standardized reference test referred to as a gold standard. However, for bovine tuberculosis (bTB), a chronic disease of cattle, affecting animal and public health, no reliable gold standard is available. In this context, latent-class models implemented using a Bayesian approach can help to assess the accuracy of diagnostic tests incorporating previous knowledge on test performance and disease prevalence. In Uruguay, bTB-prevalence has increased in the past decades partially because of the limited accuracy of the diagnostic strategy in place, based on intradermal testing (caudal fold test, CFT, for screening and comparative cervical test, CCT, for c… Leer más
Picasso C., Perez A., Gil A., Nunez A., Salaberry X., Suanses A. and Alvarez J..
The bla NDM-1 carrying IncA/C2 plasmid underlies structural alterations and co-integrate formation in vivo
Antimicrobial Agents And Chemotherapy 63(8).
In 2012, a carbapenemase-producing Salmonella (S.) Corvallis carrying a bla NDM-1 multi-resistance IncA/C2 plasmid, apart from IncHI2 and ColE-like plasmids, was detected in a wild bird in Germany. In recent broiler chicken infection study, we observed transfer of this bla NDM-1-carrying IncA/C2 plasmid to other Enterobacteriaceae Here, we focused on the stability of this plasmid and gained insight into type and frequency of its structural alterations after an in vivo passage in a broiler infection study… Leer más
Hadziabdic S., Fischer J., Borowiak M., Malorny B., Juraschek K., Kasbohrer A., Guerra B., Deneke C., Gonzalez-Zorn B. and Szabo I.
A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)
Transboundary And Emerging Diseases 1-8.
In this study, we describe the pathology of Leishmania infantum infection in naturally infected wild Leporidae and compare diagnosis of infection using histopathology, direct fluorescent antibody (DFA) assay, immunofluorescence antibody test (IFAT), and quantitative real‐time PCR (qPCR). Tissues were analysed from 52 European rabbits (Oryctolagus cuniculus) and 7 Iberian hares (Lepus granatensis) from the Community of Madrid (Spain). Our results show that L. infantum infection is associated with only minimal histopathological lesions and that L. infantum amastigotes can be detected by DFA assay in all tissues types tested, including skin. These results were confirmed by qPCR on fresh frozen tissues in 13% of rabbits and 100% of hares.… Leer más
Ortega-García MV., Salguero FJ., Rodriguez-Bertos A., Moreno I., Garcia N., Garcia-Seco T., Torre G., Dominguez L. and Dominguez M.
Circulation of a novel strain of dolphin morbillivirus (DMV) in stranded cetaceans in the Mediterranean Sea
Scientific Reports 9:9792.
Dolphin morbillivirus (DMV) has been responsible for several outbreaks of systemic infection and has resulted in cetacean strandings in the Mediterranean. In August-October 2016, seven striped dolphins (Stenella coeruleoalba) stranded on the Sicilian coastline (Italy) tested positive for DMV. Tissue samples from brain, lung, pulmonary lymph nodes, heart, spleen, liver, stomach, intestine, kidneys and urinary bladder, as well as blowhole swabs, were collected during necropsy for molecular diagnostics and pathology studies. Extracted tissue RNA was screened for DMV by real-time reverse transcription polymerase chain reaction (PCR). Some tissues exhibited microscopic lesions that were consistent with DMV infection on histopathological and immu… Leer más
Mira F., Rubio-Guerri C., Purpari G., Puleio R., Caracappa G., Gucciardi F., Russoto L., Ruggero-Loria G. and Guercio A.
Nucleotide sequence variations may be associated with virulence of deformed wing virus
Apidologie 1-15.
Western honey bees (Apis mellifera) are key players in crop pollination and in the maintenance of global biodiversity. Their viability is threatened by Varroa destructor, which acts as a vector of the deformed wing virus (DWV). Several genetic DWV variants have been reported, but it is unclear whether their virulence differs. We examined the prevalence of V. destructor and DWV as well as bee health in two colonies over 21 months and then characterizing DWV variants from each colony using phylogenetics. Colony H showed no signs of disease or mortality, and DWV sequence from this colony clustered with VDV/DWV-B sequences previously reported in healthy colonies. Colony W showed DWV symptoms, and DWV sequence clustered with DWV-A sequences prev… Leer más
Barroso-Arevalo S., Vicente-Rubiano M., Molero F., Puerta F. and Sanchez-Vizcaino JM..
Effect of litter aggregation and pooling on detection of porcine reproductive and respiratory virus in piglet processing fluids
Journal Of Veterinary Diagnostic Investigation 31(4):625-628.
A sampling technique has been validated to monitor porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) using the serosanguinous exudate known as processing fluids (PFs) that accumulate from tissues obtained during tail docking and castration. PFs are an aggregate sample of large numbers of piglets and litters. However, little is known about the effect of litter aggregation on the ability of PCR to correctly classify an aggregated PF sample as positive. We evaluated both the effect of litter aggregation and of PF pooling on PCR detection. We estimated that aggregation of at least 50 litters was possible when a pig with a Ct value of ~22 was present in the sample, and aggregation of up to 40 litters was possible when there was a s… Leer más
Vilalta C., Baker J., Sanhueza J., Murray D., Sponheim A., Alvarez J., Sylvia F., Polson D., Torremorell M., Corzo C. and Morrison RB.
The possible route of introduction of bluetongue virus serotype 3 into Sicily by windborne transportation of infected Culicoides spp
Transboundary And Emerging Diseases 66(4):1665-1673.
In October 2017, the first outbreak of bluetongue virus serotype 3 (BTV-3) began in Italy, specifically in western Sicily. The route of entrance remains unclear, although since 2016 the same strain had been circulating only 150 km away, on the Tunisian peninsula of Cape Bon. The present analysis assessed the feasibility that wind could have carried BTV-3-infected Culicoides spp. from Tunisia to Sicily. An advection-deposition-survival (ADS) model was used to estimate when and where Culicoides spp. were likely to be introduced prior to the first BTV-3 report in Italy. Additionally, the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model was used to support ADS outputs. The modelling suggests that during September 2017, st… Leer más
Aguilar-Vega C., Fernandez-Carrion E. and Sanchez-Vizcaino JM..
Potentially human-virulent Vibrio vulnificus isolates from diseased great pompano (Trachinotus goodei)
Transboundary And Emerging Diseases 66(4):1765-1770.
Vibrio vulnificus is an opportunistic human pathogen responsible for the majority of seafood-associated deaths worldwide and is also a relevant fish pathogen for the aquaculture industry. In addition to infections in aquatic livestock, Vibrio vulnificus also represents a risk to aquarium animals. For the first time, this work describes an important mortality outbreak in Trachinotus goodei in a zoo aquarium, with the isolation of Vibrio vulnificus (Vv) from the internal organs of the diseased fish. The isolates were identified by MALDI-TOF MS, serotyped and characterized by PFGE. Although the isolates from great pompanos did not belong to pathovar piscis (formerly biotype 2) or to any of the fish-related serovars, they all had identical phen… Leer más
Gibello A., Vela AI., Martinez-Nevado E., Rodriguez-Bertos A., Casamayor A., Garcia J., Dominguez L., Montoto P., Fernandez-Garayzabal JF. and Amaro C.
Host Richness Increases Tuberculosis Disease Risk in Game-Managed Areas
Microorganisms 7(6):182.
Current scientific debate addresses whether species richness in animal communities may negatively moderate pathogen transmission and disease outcome (dilution effect), or to the contrary, if disease emergence benefits from more diverse community assemblages (amplification effect). The result may not depend exclusively on patterns of host species biodiversity but may depend on the specific composition of reservoir hosts and vectors, and their ecology. Host–pathogen interactions have shaped variations in parasite virulence, transmissibility and specificity. In the same way the importance of factors related to host exposure or to life history trade-offs are expected to vary. In this study, we demonstrate that ungulate host species richness cor… Leer más
Barasona JA., Gortazar C., de la Fuente J. and Vicente J.
