Last SCI scientific publications
First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain
Veterinary Microbiology 276:109604.
Taylorella asinigenitalis is a non-pathogenic bacteria isolated from the genital tract of donkeys but also a cause of metritis and vaginal discharge in mares. It is closely related to Taylorella equigenitalis, the cause of Contagious Equine Metritis (CEM) in horses, and has been present in different countries in Europe since 1995. Up to date, there are no studies on the prevalence of T. asinigenitalis in the equine or asinine populations in Spain; this is the first report of the presence of T. asinigenitalis in donkeys (Equus asinus) from different breeds in three regions of Spain. A total of 106 healthy animals of three different Spanish donkey breeds: Andaluza (26), Majorera (12) and Zamorano-Leonés (68) were sampled between June and July… Leer más
Dorrego-Rodriguez A., Herranz-Benito C., Perez-Sancho M., Camino E., Gomez-Arrones V., Carrasco JJ., de Gabriel-Perez J., Serres C. and Cruz F..
Exploiting 16S rRNA-based metagenomics to reveal neglected microorganisms associated with infertility in breeding bulls in Spanish extensive herds
Research In Veterinary Science 150:52-57.
Bovine infectious infertility represents a problem due to the high impact on animal production and, in many cases, in public health. A lack of information on the characteristics of the bacterial population of the bovine reproductive system can hamper a comprehensive understanding of reproductive pathologies and the role that the microbiome could play. A metagenomic study based on the V3-V4 hypervariable region of the bacterial 16S rRNA gene was performed in 1029 preputial samples from bulls raised in an extensive regimen in Spain (944 from herds with low fertility rates -case group-, and 85 samples from reproductively healthy herds -control group-). The most representative phyla as well as the most 10 abundant bacterial families and their a… Leer más
Polo C., Hernandez M., Garcia-Seco T., Fernandez V., Briones V., Diez-Guerrier A., Abad D., Rodriguez-Lazaro D., Dominguez L. and Perez-Sancho M..
Identifying high-risk areas for dog-mediated rabies using Bayesian spatial regression
One Health 15:100411.
Despite ongoing control efforts, rabies remains an endemic zoonotic disease in many countries. Determining high-risk areas and the space-time patterns of rabies spread, as it relates to epidemiologically important factors, can support policymakers and program managers alike to develop evidence-based targeted surveillance and control programs. In this One Health approach which selected Thailand as the example site, the location-based risk of contracting dog-mediated rabies by both human and animal populations was quantified using a Bayesian spatial regression model. Specifically, a conditional autoregressive (CAR) Bayesian zero-inflated Poisson (ZIP) regression was fitted to the reported human and animal rabies case counts of each district, … Leer más
Kanankege K., Errecaborde KM., Wiratsudakul A., Wongnak P., Yoopatthanawong C., Thanapongtharm W., Alvarez J. and Perez A.
Animal abuse in Spanish-breed fighting cocks (Gallus gallus domesticus)
Forensic Science International 2:100050.
Cockfighting is illegal both in the European Union and the United States, although fighting cock husbandry and breed preservation are allowed. Here, we describe the pathological findings observed in the carcasses of two Spanish-breed fighting cocks submitted to our laboratory after an animal abuse-suspected death. The postmortem examination revealed these cocks may have been used for cockfighting (comb, ear lobes, wattles, and chest feather removal; and trimmed spurs). The severe extensive hemorrhages observed in the cervical region of both birds, affecting C1-C4 in bird No. 1 and C8-C11 in No. 2, suggested cervical dislocation in a non-professional manner. To the best of our knowledge, this is the first report of abuse in a Spanish-breed f… Leer más
Rebollada A., Barcena C., Barreno-San Antolin L., Pizarro M., Dominguez L. and Rodriguez-Bertos A..
Intestinal T-cell lymphoma in an Asian small-clawed otter: case report and literature review of lymphoma in the subfamily Lutrinae
Journal Of Veterinary Diagnostic Investigation 10406387221138293.
The Asian small-clawed otter (Aonyx cinereus) is an endangered species that is common in zoologic collections. A 17-y-old female Asian small-clawed otter under human care, with a clinical history of chronic renal disease, was euthanized because of deteriorating health. Histologically, the jejunal wall was infiltrated by a monomorphic population of small neoplastic lymphocytes that expanded the lamina propria of the villi and crypts, and on rare occasions invaded the submucosa. The tumor was composed of T cells (CD3+) with a proliferation index of 16%. Based on the World Health Organization (WHO) Classification of Hematopoietic Neoplasms in Domestic Animals, this lymphoma was classified as an enteropathy-associated T-cell lymphoma (EATL) typ… Leer más
Rebollada A., Canales R., Romani-Cremaschi U. and Rodriguez-Bertos A..
Effect of a recent parenteral dexamethasone and ketoprofen administration on the immunological diagnosis of tuberculosis in goats
Frontiers In Veterinary Science 1-13.
Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB eradication programmes are based mainly on intradermal tuberculin tests and slaughterhouse surveillance. Different factors may affect the performance of the TB diagnostic tests used in caprine herds and, therefore, their ability to detect infected animals. The present study evaluates the effect of the fraudulent administration of two anti-inflammatory substances, dexamethasone and ketoprofen, on the performance of the TB diagnostic techniques used in goats, as well as the suitability of high performance liquid chromatography (HPLC) for their detection in hair samples. The animals (n = 90) were distributed in three groups: (… Leer más
Ortega J., de Juan L., Sevilla IA., Garrido JM., Velasco C., Romero B., Dominguez M., Perez del Val B., Nebot C., Saez-Llorente JL., Alvarez J. and Bezos J..
P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection
Comparative Immunology, Microbiology And Infectious Diseases 90-91:101891.
The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, but without altering the negative or positive status of each sample. P22 ELISA presented 5.71 % cross-reactivity when CLA-positive sheep were evaluated,… Leer más
Doria-Barral T., Infantes-Lorenzo JA., Moreno I., de Garnica-Garcia MG., Perez del Val B., Gortazar C., Meyer B., Portela RD., Dominguez L., Dominguez M. and Balseiro A.
A subunit vaccine candidate based on the Spike protein of SARS-CoV-2 prevents infectious virus shedding in cats
Research In Veterinary Science 148:52-64.
Of the numerous animal species affected by the SARS-CoV-2 virus, cats are one of the most susceptible, and cat-to-cat transmission has been described. Although cat-to-human infection has not, as yet, been demonstrated, preventive measures should be taken in order to avoid both viral infection in cats and transmission among them. In this respect, the application of an effective vaccine to at-risk populations would be a useful tool for controlling the disease in this species. Here, we test a new vaccine prototype based on the Spike protein of the virus in order to prevent infection and infectious virus shedding in cats. The vaccine employed in experimentation, and which is easily produced, triggered a strong neutralizing antibody response in … Leer más
Barroso-Arevalo S., Sánchez-Morales L., Dominguez M., Garcia-Seco T., Risalde MA., Garcia-Bocanegra I., Dominguez L. and Sanchez-Vizcaino JM..
Increasing the data on elasmobranch plasma protein electrophoresis: electrophoretogram reference values determination in the undulate skate (Raja Undulata) and the nursehound shark (Scyliorhinus stellaris) maintained under human care
BMC Veterinary Research 18(1):380.
Background: This study determined plasma protein electrophoresis (PPE) reference intervals in two elasmobranch species: the undulate skate (Raja undulata) and the nursehound shark (Scyliorhinus stellaris), using a reference population of 48 undulate skates (27 males, 21 females) and 62 nursehounds (32 males, 30 females), considered to be clinically healthy. Plasma samples were analyzed using capillary zone electrophoresis (CZE).
Results: The undulate skate electrophoretogram resembled those previously reported in other batoids and could be divided into seven consistent fractions. No statistically significant differences were detected between sexes and developmental stages. The nursehound electrophoretogram was similar to that previou… Leer más
Moron-Elorza P., Rojo-Solis C., Steyrer C., Alvaro-Alvarez T., Valls-Torres M., Ortega J., Encinas T. and Garcia-Parraga D.
Antimicrobial resistance and One Health
Revista Espannola De Quimioterapia 35 Suppl 3:37-40.
Antimicrobial resistance is one of the major health problems we face in the 21st century. Nowadays we cannot understand global health without the interdependence between the human, animal and environmental dimensions. It is therefore logical to adopt a "One Health" approach to address this problem. In this review we show why a collaboration of all sectors and all professions is necessary in order to achieve optimal health for people, animals, plants and our environment… Leer más
Serna C. and Gonzalez-Zorn B..
