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January 1st, 2022

Trans-species transmission of Brucellae among ruminants hampering brucellosis control efforts in Egypt

Journal Of Applied Microbiology 132(1):90-100.

     This study aimed to identify the genotypic fingerprinting of B. melitensis biovar 3 isolates from ruminants in Kafr El-Sheikh, Egypt, to compare with other peers globally, and to highlight the epidemiology and potential causes of brucellosis control failure.

A multilocus variable-number tandem-repeat analysis (MLVA 16) was carried out on 41 B. melitensis bv3 isolates, 31 from the preferential hosts (28 sheep and three goats), and ten from atypical hosts (nine cattle and one buffalo), identified by bacteriological and molecular techniques. MLVA-16 analysis revealed 19 genotypes with nine as singletons. The most prevalent genotypes were M3_K.E (3,5,3,13,1,1,3,3,7,43,8,7,6,7,5,3), M13_K.E (3,5,3,13,1,1,3,3,7,43,8,5,8,7,7,3), and… Leer más

Hegazy Y., Abdel-Hamid N., Eldahiey M., Oreiby A., Algaabary M., Hamdy M., Beleta E., Martinez I., Shahein M., Garcia N. and Eltholth M.

December 1st, 2021

Genomic and Pathogenic Investigations of Streptococcus suis Serotype 7 Population derived from a Human Patient and Pigs

Emerging Microbes And Infections 1-41.

     Streptococcus suis is one of the important emerging zoonotic pathogens. Serotype 2 is most prevalent in patients worldwide. In the present study, we first isolated one S. suis serotype 7 strain GX69 from the blood culture of a patient with septicemia complicated with pneumonia in China. In order to deepen the understanding of S. suis serotype 7 population characteristics, we investigated the phylogenetic structure, genomic features, and virulence of S. suis serotype 7 population, including 35 strains and 79 genomes. Significant diversities were revealed in S. suis serotype 7 population, which were clustered into 22 sequence types (STs), five minimum core genome (MCG) groups, and six lineages. Lineages 1, 3a, and 6 were mainly constituted by… Leer más

Liang P., Wang M., Gottschalk M., Vela AI., Estrada AA., Wang J., Du P., Luo M., Wu Z. and Zheng H.

November 30th, 2021

Safety of African Swine Fever Vaccine Candidate Lv17/WB/Rie1 in Wild Boar: Overdose and Repeated Doses

Frontiers In Immunology 12:761753.

     African swine fever (ASF) is a highly lethal infectious disease that affects domestic pigs and wild boar. Outbreaks of ASF have grown considerably in the last decade causing important economic consequences for the swine industry. Its control is hampered by the lack of an effective treatment or vaccine. In Europe, the wild boar is a key wild reservoir for ASF. The results of the oral vaccination trial of wild boar with Lv17/WB/Rie1 are hope for this problem. However, this vaccine candidate has certain safety concerns, since it is a naturally attenuated vaccine. Therefore, the current study aims to evaluate the safety of this vaccine candidate in terms of overdose (high dose) and repeated doses (revaccination) in wild boar. Low-dose orally va… Leer más

Barasona JA., Cadenas-Fernández E., Kosowska A., Barroso-Arevalo S., Rivera B., Sanchez R., Porras-Gonzalez N., Gallardo C. and Sanchez-Vizcaino JM..

November 30th, 2021

Evolution of ColE1-like plasmids across γ-Proteobacteria: From bacteriocin production to antimicrobial resistance

PLoS Genetics 17(11):e1009919.

     Antimicrobial resistance is one of the major threats to Public Health worldwide. Understanding the transfer and maintenance of antimicrobial resistance genes mediated by mobile genetic elements is thus urgent. In this work, we focus on the ColE1-like plasmid family, whose distinctive replication and multicopy nature has given rise to key discoveries and tools in molecular biology. Despite being massively used, the hosts, functions, and evolutionary history of these plasmids remain poorly known. Here, we built specific Hidden Markov Model(HMM) profiles to search ColE1 replicons within genomes. We identified 1,035 ColE1 plasmids in five Orders of γ-Proteobacteria, several of which are described here for the first time. The phylogenetic a… Leer más

Ares M., Rocha EPC. and Gonzalez-Zorn B..

November 20th, 2021

Differences in wild boar spatial behaviour among land uses and management scenarios in Mediterranean ecosystems

Science Of The Total Environment 796:148966.

     The ubiquitous wild boar is causing diverse and growing conflicts of socio-ecological and economic relevance worldwide. For that reason, knowledge of its spatial ecology is crucial to designing effective management programmes. But this knowledge is scarce in Mediterranean areas with mixed land uses. We describe the spatial ecology and habitat selection of 41 adult wild boar monitored using GPS collars and analyse the effects of sex and the period (food shortage period, hunting season and food abundance period) under different land uses (protected areas, mixed farms and fenced hunting estates). The spatial ecology of wild boar was characterised by marked temporality, mediated by sex and the land uses in the area. The activity (ACT), daily ra… Leer más

Laguna E., Barasona JA., Vicente J., Keuling O. and Acevedo O.

November 3rd, 2021

Bottlenose dolphins (Tursiops truncatus) aggressive behavior towards other cetacean species in the western Mediterranean

Scientific Reports 11(1):21582.

     Aggressive behavior of bottlenose dolphins (Tursiops truncatus) towards conspecifics is widely described, but they have also often been reported attacking and killing harbour porpoises (Phocoena phocoena) around the world. However, very few reports exist of aggressive interactions between bottlenose dolphins and other cetacean species. Here, we provide the first evidence that bottlenose dolphins in the western Mediterranean exhibit aggressive behavior towards both striped dolphins (Stenella coeruleoalba) and Risso`s dolphins (Grampus griseus). Necropsies and visual examination of stranded striped (14) and Risso`s (2) dolphins showed numerous lesions (external rake marks and different bone fractures or internal organ damage by blunt trauma).… Leer más

Crespo JL., Rubio-Guerri C., Jimenez MA., Aznar JF., Marco-Cabedo V., Melero M., Sanchez-Vizcaino JM., Gozalbes P. and Garcia-Parraga D.

November 3rd, 2021

A new method for sampling African swine fever virus genome and its inactivation in environmental samples

Scientific Reports 11(1):21560.

     African swine fever (ASF) is currently the most dangerous disease for the global pig industry, causing huge economic losses, due to the lack of effective vaccine or treatment. Only the early detection of ASF virus (ASFV) and proper biosecurity measures are effective to reduce the viral expansion. One of the most widely recognized risks as regards the introduction ASFV into a country is infected animals and contaminated livestock vehicles. In order to improve ASF surveillance, we have assessed the capacity for the detection and inactivation of ASFV genome by using Dry-Sponges (3 M) pre-hydrated with a new surfactant liquid. We sampled different surfaces in ASFV-contaminated facilities, including animal skins, and the results were compared to… Leer más

Kosowska A., Barasona JA., Barroso-Arevalo S., Rivera B., Dominguez L. and Sanchez-Vizcaino JM..

November 1st, 2021

Large-scale study on virological and serological prevalence of SARS-CoV-2 in cats and dogs in Spain

Transboundary And Emerging Diseases 1-16.

     The disease produced by the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), is currently one of the primary concerns worldwide. Knowing the zoonotic origin of the disease and that several animal species, including dogs and cats, are susceptible to viral infection, it is critical to assess the relevance of pets in this pandemic. Here, we performed a large-scale study on SARS-CoV-2 serological and viral prevalence in cats and dogs in Spain in order to elucidate their role and susceptibility. Samples from animals in contact with COVID-19 positive people and/or compatible symptoms (n = 492), as well as from random animals (n = 1024), were taken. Despite the large number of animals analyzed, only 12 animals (eight dogs and … Leer más

Barroso-Arevalo S., Barneto A., Ramos AM., Rivera B., Sanchez R., Sanchez-Morales I., Perez-Sancho M., Buendia A., Ferreras E., Ortiz-Menendez JC., Moreno I., Serres C., Vela C., Risalde MA., Dominguez L. and Sanchez-Vizcaino JM..

November 1st, 2021

Equine infection with Leishmania spp. in Costa Rica: Study of five cases

Veterinary Medicine And Science 7(6):2234-2239.

     Cutaneous forms of leishmaniosis due to Leishmania braziliensis have been reported in horses in the New World. Domestic animals play a role in the transmission of the disease. In Costa Rica, human cases of L. braziliensis, L. panamensis and L. infantum have been reported. The present report describes five cases of equine cutaneous leishmaniosis in Costa Rica. The aetiological diagnosis was based on the presence of the parasite within the lesions. Skin biopsies were used to perform histopathological analyses of the lesions. Immunohistochemistry was used to detect the presence of the Leishmania spp. antigens in tissue sections. Laser-capture micro-dissection and quantitative real-time PCR techniques were carried out to detect the pathogen nuc… Leer más

Ortega-García MV., Salguero FJ., Garcia N., Dominguez M., Moreno I. and Berrocal A.

October 28th, 2021

Systematic Determination of Herpesvirus in Free-Ranging Cetaceans Stranded in the Western Mediterranean: Tissue Tropism and Associated Lesions

Viruses-Basel 13(11).

     The monitoring of herpesvirus infection provides useful information when assessing marine mammals’ health. This paper shows the prevalence of herpesvirus infection (80.85%) in 47 cetaceans stranded on the coast of the Valencian Community, Spain. Of the 966 tissues evaluated, 121 tested positive when employing nested-PCR (12.53%). The largest proportion of herpesvirus-positive tissue samples was in the reproductive system, nervous system, and tegument. Herpesvirus was more prevalent in females, juveniles, and calves. More than half the DNA PCR positive tissues contained herpesvirus RNA, indicating the presence of actively replicating virus. This RNA was most frequently found in neonates. Fourteen unique sequences were identified. Most amplif… Leer más

Vargas-Castro I., Melero M., Crespo JL., Jimenez MA., Sierra E., Rubio-Guerri C., Arbelo M., Fernandez A., Garcia-Parraga D. and Sanchez-Vizcaino JM..

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 230 ZTA 62 SUAT 14
2 ICM 208 SUAT 61 ZTA 13
3 MYC 200 MYC 53 MYC 6
4 SUAT 191 ICM 33 ICM 4
  VISAVET 979 303 57

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.802 ZTA 4.813 ZTA 5.649
2 SAP 3.238 SAP 3.713 SUAT 4.454
3 DICM 2.776 SUAT 3.402 NED 3.576
4 SUAT 2.767 NED 3.314 MYC 3.451
5 EPI 2.67 ICM 2.884 SEVISEQ 3.102
VISAVET 2.992 3.788 4.269

ISI Scientific Publications
Total Last 60 mo.
1 Transbound. Emerg. Dis. 86 Transbound. Emerg. Dis. 47
2 Vet. Microbiol. 84 Front Vet Sci 39
3 Prev. Vet. Med. 73 Prev. Vet. Med. 21
4 PLoS ONE 54 PLoS ONE 20
5 Res. Vet. Sci. 47 Res. Vet. Sci. 17
  All journals 333   All journals 36