Last SCI scientific publications
Effects on Intestinal Mucosal Morphology, Productive Parameters and Microbiota Composition after Supplementation with Fermented Defatted Alperujo (FDA) in Laying Hens
Antibiotics 8:215.
The olive oil sector is currently adapting its traditional function to also become a supplier of high-value by-products that possess antioxidant, anti-inflammatory and antimicrobial properties. In this study, we evaluated the effect of the fermented defatted alperujo (FDA) on the intestinal health of laying hens. The morphology of the duodenal and cecal mucosa, the composition of the intestinal
microbiota and the productivity of a batch of laying hens were evaluated after FDA supplementation. At early life stages, significant differences (p < 0.001) were observed in duodenal villi height and in crypt depth of both the duodenum and the cecum in the FDA-supplemented group, indicating improved intestinal health in this group. Microbiota… Leer más
Rebollada A., Barcena C., Ugarte-Ruiz M., Porras-Gonzalez N., Mayoral-Alegre F., Tome-Sanchez I., Dominguez L. and Rodriguez-Bertos A..
Coffee Silverskin Extract: Nutritional Value, Safety and Effect on Key Biological Functions
Nutrients 11(11).
This study aimed to complete the scientific basis for the validation of a coffee silverskin extract (CSE) as a novel food ingredient according to European legislation. Nutritional value, safety, effects on biochemical biomarkers and excretion of short chain fatty acids (SCFAs) in vivo of CSE were assessed. Proteins, amino acids, fat, fatty acids, fiber, simple sugars and micronutrients were analyzed. For the first time, toxicological and physiological effects were evaluated in vivo by a repeated-dose study in healthy Wistar rats. Hormone secretion, antioxidant (enzymatic and no-enzymatic) and anti-inflammatory biomarkers, and dietary fiber fermentability of CSE (analysis of SCFAs in feces) were studied in biological samples. This unique res… Leer más
Iriondo-Dehond A., Rios MB., Herrera T., Rodriguez-Bertos A., Nunez F., San Andres MI., Sanchez-Fortun S. and del Castillo MD.
Role of Wild Boar in the Spread of Classical Swine Fever in Japan
Pathogens 8(4).
Since September 2018, nearly 900 notifications of classical swine fever (CSF) have been reported in Gifu Prefecture (Japan) affecting domestic pig and wild boar by the end of August 2019. To determine the epidemiological characteristics of its spread, a spatio-temporal analysis was performed using actual field data on the current epidemic. The spatial study, based on standard deviational ellipses of official CSF notifications, showed that the disease likely spread to the northeast part of the prefecture. A maximum significant spatial association estimated between CSF notifications was 23 km by the multi-distance spatial cluster analysis. A space-time permutation analysis identified two significant clusters with an approximate radius of 12 a… Leer más
Ito S., Jurado C., Bosch J., Ito M., Sanchez-Vizcaino JM., Isoda N. and Sakoda AY.
Successful Host Adaptation of IncK2 Plasmids
Frontiers In Microbiology 10:2384.
The IncK plasmid group can be divided into two separate lineages named IncK1 and IncK2. IncK2 is found predominantly in poultry while IncK1 was reported in various mammals, including animals and humans. The physiological basis of this distinction is not known. In this manuscript we examined fitness cost of IncK1 and IncK2 plasmids at 37 and 42°C, which resembles mammalian and chicken body temperatures, respectively. We analyzed conjugation frequency, plasmid copy number and plasmid fitness cost in direct competition. Additionally, we measured levels of σ-32 in Escherichia coli carrying either wild type or conjugation-deficient IncK plasmids. The results show that IncK2 plasmids have a higher conjugation frequency and lower copy number … Leer más
Rozwandowicz M., Brouwer MSM., Mughini-Gras L., Wagenaar J., Gonzalez-Zorn B., Mevius DJ. and Hordijk J.
Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis
Veterinary Research 50:82.
Effective vaccines against tuberculosis (TB) are needed in order to prevent TB transmission in human and animal populations. Evaluation of TB vaccines may be facilitated by using reliable animal models that mimic host pathophysiology and natural transmission of the disease as closely as possible. In this study, we evaluated the immunogenicity and efficacy of two attenuated vaccines, BCG and MTBVAC, after each was given to 17 goats (2 months old) and then exposed for 9 months to goats infected with M. caprae. In general, MTBVAC-vaccinated goats showed higher interferon-gamma release than BCG vaccinated goats in response to bovine protein purified derivative and ESAT-6/CFP-10 antigens and the response was significantly higher than that observ… Leer más
Roy A., Tome-Sanchez I., Romero B., Lorente-Leal V., Infantes-Lorenzo JA., Dominguez M., Martin C., Aguilo N., Puentes E., Rodriguez E., de Juan L., Risalde MA., Gortazar C., Dominguez L. and Bezos J..
Risk of African swine fever virus introduction into the United States through smuggling of pork in air passenger luggage
Scientific Reports 9(1):14423.
African swine fever causes substantial economic losses in the swine industry in affected countries. Traditionally confined to Africa with only occasional incursions into other regions, ASF began spreading into Caucasian countries and Eastern Europe in 2007, followed by Western Europe and Asia in 2018. Such a dramatic change in the global epidemiology of ASF has resulted in concerns that the disease may continue to spread into disease-free regions such as the US. In this study, we estimated the risk of introduction of ASF virus into the US through smuggling of pork in air passenger luggage. Results suggest that the mean risk of ASFV introduction into the US via this route has increased by 183.33% from the risk estimated before the disease ha… Leer más
Jurado C., Mur L., Perez-Aguirreburualde MS., Cadenas-Fernández E., Martinez-Lopez B., Sanchez-Vizcaino JM. and Perez A.
Tuberculosis vaccination sequence effect on protection in wild boar
Comparative Immunology, Microbiology And Infectious Diseases 66:101329.
The Eurasian wild boar (Sus scrofa) is a reservoir for tuberculosis (TB) in which vaccination is a valuable tool for control. We evaluated the protection and immune response achieved by homologous and heterologous regimes administering BCG and heat-inactivated Mycobacterium bovis (IV). Twenty-one wild boar piglets were randomly allocated in five groups: Control, homologous BCG, homologous IV, heterologous IV-BCG, heterologous BCG-IV. Significant 67% and 66% total lesion score reductions were detected in homologous IV (IVx2) and heterologous IV-BCG groups when compared with Control group (F4,16 = 6.393, p = 0.003; Bonferroni Control vs IVx2 p = 0.026, Tukey Control vs IV-BCG p = 0.021). No sign… Leer más
Diez-Delgado I., Sevilla IA., Garrido JM., Romero B., Geijo MV., Dominguez L., Juste RA., Aranaz A., de la Fuente J. and Gortazar C.
Risk of acquisition of human diarrhoeagenic Escherichia coli virulence genes in intercontinental travellers: a prospective, multi-centre study Travel Medicine and Infectious Disease
Travel Medicine And Infectious Disease 31:101362.
Background:
We studied the geographic distribution of diarrhoeagenic Escherichia coli virulence genes (DEC VGs) acquisition in travellers and investigated if they acquired highly virulent EAEC/STEC hybrid strains.
Methods:
From the prospective, multicentre COMBAT study among 2,001 Dutch travellers, a subset of 491 travellers was were selected based on travel destination to 7 subregions. Faecal samples taken directly before and after travel were screened for nine DEC VGs with real-time PCR. Incidence proportions and rates were calculated for each gene and subregion.
Results:
479 travellers were analysed. 21.8% acquired aggR (EAEC), with the highest acquisition rates in Northern and Western Africa and 15.3% … Leer más
Van Hattem J.M., Cabal A., Arcilla M.S., Alvarez J., de Jong M.D., Melles D.C., Penders J., COMBAT Consortium., Gortazar C. and Schultsz C.
Mapping changes in the spatiotemporal distribution of lumpy skin disease virus
Transboundary And Emerging Diseases 66(5):2045-2057.
Lumpy skin disease virus (LSDV) is an infectious disease of cattle transmitted by arthropod vectors which results in substantial economic losses due to impact on production efficiency and profitability, and represents an emerging threat to international trade of livestock products and live animals. Since 2015, the disease has spread into the Northern Hemisphere including Azerbaijan, Kazakhstan, the Russian Federation and the Balkans. The rapid expansion of LSDV in those regions represented the emergence of the virus in more temperate regions than those in which LSDV traditionally occurred. The goal of this study was to assess the risk for further LSDV spread through the a) analysis of environmental factors conducive for LSDV, and b) estimat… Leer más
Machado G., Korennoy FI., Alvarez J., Picasso C., Perez A. and VanderWaal K.
Emergent subtype of hepatitis E virus genotype 3 in wild boar in Spain
Transboundary And Emerging Diseases 66(5):1803-1808.
Wild boar (Sus scrofa) is considered the main wildlife reservoir of zoonotic hepatitis E virus (HEV) genotypes. The aim of this study was to evaluate the circulation of HEV in free-ranging wild boar in the Doñana National Park (DNP), Spain. Blood samples were collected from 99 wild boar in the DNP during 2015. Sera were analyzed in parallel using indirect ELISA and real-time RT-PCR. A total of 57 of the 99 tested animals (57.6%; 95%CI: 47.8-67.3%) had anti-HEV antibodies, indicating that this virus is widespread in wild boar in the DNP. HEV RNA was detected in one animal and phylogenetic analysis showed that the sequence isolated belonged to subtype 3r. The results suggest a potential risk of zoonotic transmission of this novel HEV-3 subtyp… Leer más
Caballero-Gomez J., Jiménez-Ruiz S., Lopez-Lopez P., Vicente J., Risalde MA., Cano-Terriza D., Frias M., Barasona JA., Rivero A., Garcia-Bocanegra I. and Rivero-Juarez A.
