SCI scientific publications 1991
Transformation of sterigmatocystin and O-methylsterigmatocystin by aflatoxigenic and nonaflatoxigenic field isolates of the Aspergillus flavus group
Mycopathologia 116(2):71-5.
Transformation of sterigmatocystin and O-methylsterigmatocystin (two metabolic aflatoxin precursors) to aflatoxins by aflatoxigenic and nonaflatoxigenic field isolates of Aspergillus flavus was studied. The 24 nonaflatoxigenic isolates investigated failed to transform both precursors. Among the 8 aflatoxin-producing isolated used, 7 transformed both precursors whereas the remaining failed to transform both. According to these results, the usefulness of the measurement of enzymatic activities related to aflatoxin production in understanding the true status of conflictive field isolates is discussed… Leer más
Pro MJ., Moreno MA. and Suarez G.
Detection of staphylococcal enterotoxin and toxic shock syndrome toxin-1 (TSST-1) by immunoblot combined with a semiautomated electrophoresis system
Journal Of Immunological Methods 144(2):197-202.
An immunoblot technique combined with a semi-automated electrophoresis system has been developed for the detection of staphylococcal enterotoxins and toxic shock syndrome toxin-1 (TSST-1). The advantages of this method over other detection techniques include speed, sensitivity (10 ng/ml) and specificity. The use of semiautomated electrophoresis permits the routine detection of staphylococcal enterotoxins and TSST-1… Leer más
Orden JA., Goyache J., Hernandez FJ., Domenech A., Suarez G. and Gomez-Lucia E.
Pathogenesis of lymphoid lesions in murine experimental listeriosis
Journal Of Comparative Pathology 105(1):1-15.
Adult female Swiss albino mice were infected intraperitoneally or subcutaneously with Listeria monocytogenes Serovar 4b or 1/2a and killed at intervals. Thymus, spleen, Peyer`s patches and a variety of lymph nodes, including the jejunal (mesenteric), mediastinal, lumbar, mandibular and superficial inguinal, were examined by histopathology and by immunocytochemistry for detection of L. monocytogenes antigen. Similar results were obtained with both Serovars and by both routes of inoculation used. In the spleen, L. monocytogenes was detected, by immunoperoxidase staining, as soon as 4 h after inoculation, inside phagocytic cells located predominantly in the marginal zone of the white pulp. This was followed by inflammation, necrosis and deplet… Leer más
Marco A., Domingo M., Prats M., Briones V., Pumarola M. and Dominguez L..
Different media and methodologies for the detection of aflatoxin production by Aspergillus flavus strains isolated from trout feed
Mycopathologia 113(2):121-5.
We have studied the aflatoxin producing capacity of 41 Aspergillus flavus strains isolated from the mycoflora present of natural media (wheat, rice and mixed feed) synthetic medium (Aflatoxin Producing Ability Medium) and semisynthetic media (Coconut Agar Medium and Glucose Yeast Extract Agar) were compared. Aflatoxins were analysed on days 4 and 8 post-inoculation under an incubation temperature of 28 degrees C. A total of 30 strains (75.7%) were producers on natural media as detected by Thin Layer Chromatography: 23 strains on wheat, 27 on rice and 12 on mixed feed. The results by qualitative fluorescence tests on synthetic and semisynthetic media were: 3 strains positive on Coconut Agar Medium (CAM) 1 on Glucose Yeast Extract Agar (GY + … Leer más
Cutuli MT., Cuellar A., Camara M., Mateos A. and Suarez G.
