Scientific publications 1994

Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans

FEMS Microbiology Letters 124(1):35-41.

     A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants… Leer más

Moreno MA., Pascual C., Gibello A., Ferrer S., Bos CJ., Debets AJ. and Suarez G.

Beak infection by Penicillium cyclopium in a macaw (Ara ararauna)

Avian Diseases 38(4):922-7.

     A case of localized beak infection by Penicillium cyclopium in a macaw (Ara ararauna) is described. A necrotic lesion, with destruction of the corneous layer, was localized in the upper zone of the beak. Diagnosis was carried out on samples of the affected zone by direct microscopic observation, routine fungal culturing techniques, and scanning electron microscopy. Results were consistent: P. cyclopium was the only microorganism associated with the lesion site. No previous reports concerning this type of beak pathology have been found in the literature… Leer más

Bengoa A., Briones V., Lopez MB. and Paya MJ.

Survey of patulin in apple juice and children`s apple food by the diphasic dialysis membrane procedure

Journal Of Agricultural And Food Chemistry 42:1701-1703.

     A survey of patulin in 100 samples of commercial apple juice and 12 samples of apple food for children collected at stores in Madrid (Spain) during 1992 was conducted.… Leer más

Prieta J., Moreno MA., Diaz S., Suarez G. and Dominguez L..

Concurrent infection with Streptococcus equisimilis and Leishmania in a dog

Journal Of Veterinary Diagnostic Investigation 6(3):371-5.

Ramos-Vara JA., Briones V., Segales J., Vilafranca M., Sorde A. and Miller MA.

Susceptibilities of Listeria species isolated from food to nine antimicrobial agents

Antimicrobial Agents And Chemotherapy 38(7):1655-7.

     The agar dilution method was used to determine the activities of gentamicin, erythromycin, streptomycin, chloramphenicol, ampicillin, sulfamethazine, cephalothin, penicillin G, and tetracycline against 73 strains belonging to the genus Listeria (L. innocua, L. seeligeri, and L. monocytogenes). All strains were isolated from raw milk, cheese, the dairy processing plant, poultry, and the poultry slaughterhouse. Gentamicin, ampicillin, and erythromycin, of which the MICs for 90% of the strains tested for all three species were < or = 5.96 micrograms/ml, were found to be the most active agents studied. Most of the L. innocua strains isolated from poultry and the poultry slaughterhouse were resistant to tetracycline… Leer más

Franco CM., Quinto EJ., Fente C., Rodriguez JL., Dominguez L. and Cepeda A.

Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B

Journal Of Veterinary Medicine. B, Infectious Diseases And Veterinary Public Health 41(10):639-44.

     Three monoclonal antibodies (MAbs) which react specifically with staphylococcal enterotoxin B (SEB) were studied for their suitability for use in ELISA. One of them (MAb B14) worked well as a coating antibody; MAb B12 was shown to be a good probing antibody (conjugated with peroxidase) when ELISA plates were coated with MAb B14. This effective pair of MAbs (B14-B12PO) is able to detect 0.625 ng SEB ml, and to distinguish between SEB and other proteins present in food extracts… Leer más

Goyache J., Orden JA., Blanco JL., Domenech A., Suarez G. and Gomez-Lucia E.

Chloramphenicol extraction from milk by using the diphasic dialysis method followed by liquid chromatographic determination

Journal Of AOAC International 77(4):854-6.

     An economical, fast, and simple method for the extraction and liquid chromatographic determination of chloramphenicol (CAP) in milk is described. CAP is extracted by using a recently developed membrane-based method named "diphasic dialysis." CAP is detected and quantitated in the organic solvent used in dialysis without additional cleanup steps by reversed-phase liquid chromatography and UV detection (270 nm). The determination limit of CAP in milk was about 5 micrograms/L, although as little as 1 microgram/L could be detected under optimal working conditions… Leer más

Bayo FJ., Moreno MA., Prieta J., Diaz S., Suarez G. and Dominguez L..