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SCI scientific publications 2010

December 31st, 2010

Utilization of lactose and presence of the phospho-B-galactosidase (lacG) gene in Lactococcus garvieae isolates from different sources

International Microbiology 13(4):189-93.

     This study evaluates the utilization of lactose (Lac) and the presence of the phospho-B-galactosidase (lacG) gene as markers for distinguishing between fish (Lac-/lacG-) and dairy isolates (Lac+/lacG+) of Lactococcus garvieae, using a panel of L. garvieae isolates from different sources. None of the fish isolates produced acid from lactose (Lac-), however Lac-/lacG- isolates were observed in pigs, cows, birds and humans. Most of the dairy isolates (77.8%) were Lac+/lacG+, but some dairy isolates did not produce acid from this sugar. Data in the present study show that the ability to metabolize lactose and the presence of the lacG gene are heterogeneously scattered among L. garvieae isolates of different sources. Therefore, the use of these … Leer más

Aguado-Urda M., Cutuli MT., Blanco MM., Aspiroz C., Tejedor JL., Fernandez-Garayzabal JF. and Gibello A.

December 15th, 2010

Ante-mortem testing wild fallow deer for bovine tuberculosis

Veterinary Microbiology 146(3-4):285-9.

     This study aimed to maximize the sensitivity of bovine tuberculosis detection in living wild fallow deer (Dama dama) under field conditions. We evaluated the rapid test (RT; CervidTB STAT-PAK Assay, Chembio Diagnostic Systems, Inc., USA) in comparison with the comparative cervical skin test (CCT). A total of 134 fallow deer were captured between January and March 2008. At time 0, 0.1 ml of avian purified protein derivative (avian PPD; Cooper-Zeltia, Spain), 0.1 ml bovine PPD (Cooper-Zeltia, Spain), 0.1 ml negative control PBS and 0.1 ml of a positive control (the mitogen phytohaemagglutinin, PHA; containing 250 mg PHA, diluted in PBS) were injected intradermally at four shaved sites in the neck. The skin fold thickness at each injection sit… Leer más

Jaroso R., Vicente J., Martin-Hernando MP., Aranaz A., Lyashchenko KP., Greenwald R., Esfandiari J. and Gortazar C.

December 1st, 2010

Prudent use of antimicrobial agents: not just for humans

Enfermedades Infecciosas Y Microbiología Clínica 28(10):669-71.

     The use of antimicrobial agents (AA) is required for the treatment of infectious diseases, but, at the same time, is the main cause for the selection of antimicrobial resistant bacteria. The wide use of AA in human medicine is a key cause of this problem, but not the only one, as other ‘‘non-human’’ uses of AA are also contributing to the alarming increase of resistant bacteria.… Leer más

Torres C., Moreno MA. and Zarazaga M.

December 1st, 2010

Quantification of the risk for introduction of virulent Newcastle disease virus into Spain through legal trade of live poultry from European Union countries

Avian Pathology: Journal Of The W.V.P.A 39(6):459-65.

     Newcastle disease (ND) causes large economic losses in poultry production worldwide. Spain has reported two ND epidemics in poultry farms since 1993, the most recent in 2009. The recent increase in the number of ND epidemics reported in Spain and in other European Union (EU) member countries along with the failure to identify the source of the Spanish epidemics caused concern over the vulnerability that Spain has to the disease. Some of the epidemics recently reported in EU member states were associated with legal introduction of live poultry; the large number of susceptible species annually imported by Spain from the EU suggests that legal imports of poultry may impose a risk for the introduction of virulent ND virus (v-NDV) into the count… Leer más

Sanchez-Vizcaino F., Perez AM., Lainez M. and Sanchez-Vizcaino JM..

December 1st, 2010

Genetic analysis of Streptococcus suis isolates recovered from diseased and healthy carrier pigs at different stages of production on a pig farm

The Veterinary Journal 186(3):396-8.

     Streptococcus suis isolates from pigs at different stages of production on a farrow-to-finish farm were characterised by serotyping, pulsed-field gel electrophoresis (PFGE) and production of muramidase-released protein, extracellular factor and suilysin. S. suis was isolated from the tonsils of 81/287 (28.2%) healthy pigs: 16/47 (34%) post-weaning, 18/47 (38.3%) transition, 18/47 (38.3%) fattening and 29/146 (19.9%) sows. A total of 127 S. suis isolates were analysed: 14 from diseased pigs at the post-weaning stage and 113 from the tonsils of healthy pigs. Serotypes 2, 4, 9, 14 and 1/14 were isolated from both diseased and healthy pigs. A total of 83 PFGE profiles were obtained; most isolates (95.2%) were grouped into three clusters (A–C). … Leer más

Luque I., Blume V., Borge C., Vela AI., Perea JA., Marquez JM., Fernandez-Garayzabal JF. and Tarradas C.

November 1st, 2010

Prudent use of antimicrobial agents and proposals for improvement in veterinary medicine

Enfermedades Infecciosas Y Microbiología Clínica 28(Supl.4):40-4.

     The alarms that have sounded in the last two decades of the 20th century in the field of antimicrobial resistance in livestock farming have led to the development of various measures that have substantially improved the use of antimicrobial agents in veterinary medicine. These measures mainly involve the implementation of new pharmaceutical legislation and the hygiene package provisions.
Even though awareness of the regulations is high, surveillance systems have been implemented and the promotion of appropriate use has begun to show results, it is necessary to maintain these measures while promoting alternatives to the use of antimicrobials. In particular, alternatives are needed for those agents used for prevention of bacterial dise… Leer más

Dominguez L., Moreno MA., Porrero MC. and Tellez S.

November 1st, 2010

Mass vaccination as a complementary tool in the control of a severe outbreak of Bovine Brucellosis due to Brucella abortus in Extremadura, Spain

Preventive Veterinary Medicine 97(2):119-125.

     We report the evolution of an outbreak of bovine brucellosis (Brucella abortus) in the region of Extremadura (Spain) involving more than 1000 herds and nearly 40000 animals. S19 vaccination of young cattle combined with a test and slaughter strategy did not result in a rapid decrease in herd prevalence and animal incidence; these parameters showed a constant decreasing trend only when a combination of restriction of cattle movements, increased test frequency,S19 vaccination and mass RB51 vaccination (with yearly revaccinations) were applied to all susceptible populations. These measures were applied for five years; abortions following RB51 vaccination of pregnant cows were limited to the first inoculation and the involvement of the vaccine … Leer más

Sanz C., Saez-Llorente JL., Alvarez J., Cortes M., Pereira G., Reyes A., Rubio F., Martin J., Garcia N., Dominguez L., Hermoso-de-Mendoza M. and Hermoso-de-Mendoza J.

November 1st, 2010

PCR amplification and high resolution melt curve analysis as a rapid diagnostic method to genotype members of the Mycobacterium avium-intracellulare complex

Clinical Microbiology And Infection 16:1658-1662.

     Some of the members of the Mycobacterium avium-intracellulare (MAI) complex are recognised as human pathogens in both immunocompromised and immunocompetent patients. The current molecular methods that are available to genotype the MAI complex members can be both expensive and technically demanding. In this report, we describe for the first time the application of a real-time PCR and high resolution melt approach to differentiate between the complex members by targeting a member of the PPE (Pro-Pro-Glu) gene family, MACPPE24. In this aim, reference strains of the Mycobacterium avium subspecies and Mycobacterium intracellulare (M. intracellulare) were used to optimise the technique. Then, this real time PCR-HRM approach was used to distinguis… Leer más

Castellanos E., Aranaz A. and De Buck J.

October 1st, 2010

Moraxella porci sp. nov., a new species isolated from pigs

International Journal Of Systematic And Evolutionary Microbiology 60(Pt 10):2446-50.

     Nine Gram-negative, catalase- and oxidase-positive coccus-shaped bacteria were isolated from pigs affected of different pathological processes. Phenotypic and genotypic methods were performed to determine the relationships of these isolates to species belonging to the genus Moraxella. Analysis of the 16S rRNA gene sequence demonstrated that the clinical isolates represent a new sub-line within the genus Moraxella. The isolates were closely related to Moraxella cuniculi and Moraxella pluranimalium with a 16S rRNA gene sequence similarity of 98.1% and 99.1%, respectively. The isolates displayed DNA-DNA relative binding ratios to each other of 74% but distinctly lower levels of DNA hybridization with their closely phylogenetically related mora… Leer más

Vela AI., Sanchez-Porro C., Aragon V., Olvera A., Dominguez L., Ventosa A. and Fernandez-Garayzabal JF..

September 28th, 2010

Experimental infection of European red deer (Cervus elaphus) with bluetongue virus serotypes 1 and 8

Veterinary Microbiology 145(1-2):148-52.

     Bluetongue (BT) is a climate change-related emerging infectious disease in Europe. Outbreaks of serotypes 1, 2, 4, 6, 8, 9, 11, and 16 are challenging Central and Western Europe since 1998. Measures to control or eradicate bluetongue virus (BTV) from Europe have been implemented, including movement restrictions and vaccination of domestic BTV-susceptible ruminants. However, these measures are difficult to apply in wild free-ranging hosts of the virus, like red deer (Cervus elaphus), which could play a role in the still unclear epidemiology of BT in Europe. We show for the first time that BTV RNA can be detected in European red deer blood for long periods, comparable to those of domestic ruminants, after experimental infection with BTV-1 and… Leer más

Lopez-Olvera JR., Falconi C., Fernandez-Pacheco P., Fernandez-Pinero J., Sanchez MA., Palma A., Herruzo I., Vicente J., Jimenez-Clavero MA., Arias M., Sanchez-Vizcaino JM. and Gortazar C.

September 1st, 2010

Bluetongue vaccination in Europe

Expert Review Of Vaccines 9(9):989-91.

     Bluetongue virus (BTV) is the cause of blue¬tongue (BT), an insect-transmitted disease of domestic and wild ruminants. BTV infection occurs throughout many of the temperate and tropical regions of the world, coincident with the distribution of specific species of Culicoides biting midges that act as biological vectors of the virus. BT typi¬cally occurs when susceptible animal spe¬cies are introduced into areas where virulent strains of BTV circulate, or when virulent strains of BTV extend their range into previ¬ously unexposed populations of ruminants. Following the recent northern European epidemic, BTV has spread far beyond the historical northern limits of its range.

Bluetongue virus is the prototype member of the genus Or… Leer más

Zientara S., MacLachlan NJ., Calistri P., Sanchez-Vizcaino JM. and Savini G.

September 1st, 2010

Characterization of Some Bacterial Strains Isolated from Animal Clinical Materials and Identified as Corynebacterium xerosis by Molecular Biological Techniques

Journal Of Clinical Microbiology 48(9):3138-45.

     Eighteen C. xerosis strains isolated from different animal clinical specimens were subjected to phenotypic and molecular genetic studies. On the basis of the results of the biochemical characterization, the strains were tentatively identified as C. xerosis. Phylogenetic analysis based on comparative analysis of the sequences of 16S rRNA and rpoB genes revealed that the 18 strains were highly related to C. xerosis, C. amycolatum, C. freneyi and C. hansenii. There was a good concordance between 16S rRNA and partial rpoB gene sequencing results, although partial rpoB gene sequencing allowed a better differentiation of C. xerosis. Alternatively, C. xerosis was also differentiated from C. freneyi and C. amycolatum by RFLP analysis of the 16S-23S… Leer más

Palacios L., Vela AI., Molin K., Fernandez A., Latre MV., Chacon G., Falsen E. and Fernandez-Garayzabal JF..

September 1st, 2010

Eurasian wild boar response to skin-testing with mycobacterial and non-mycobacterial antigens

Preventive Veterinary Medicine 96(3-4):211-217.

     Eurasian wild boar (Sus scrofa) are able to maintain bovine tuberculosis (bTB) in the wild and are most probably able to transmit the disease to other species, thus acting as a true wildlife reservoir. Translocation of wild boar is a common practice in European countries. Therefore, identifying effective tools for bTB diagnosis in living wild boar is crucial for the implementation of control measures. We describe for the first time the sex and origin related differences in the skin-test response to mycobacterial antigens (bPPD and aPPD) and to a non-mycobacterial antigen (PHA, a plant derived mitogen) in wild and farmed wild boar, and used a small sample of known M. bovis infected wild boar to establish whether skin-testing is an option for… Leer más

Jaroso R., Vicente J., Fernandez de Mera IG., Aranaz A. and Gortazar C.

August 28th, 2010

Isolation of Enterococcus hirae from suckling rabbits with diarrhoea

The Veterinary Record 167(9):345-346.

     Enterococci are commensal microorganisms of the intestinal tract of human beings and animals, and can also be found in a wide range of environments (Klein 2003, Donskey 2004). Enterococci were considered to be non-pathogenic for many years, but several species, most notably Enterococcus faecium and Enterococcus faecalis, have emerged as human pathogens, and are currently considered to be among the most important nosocomial pathogens (Murray and Weinstock 1999). Enterococci are also responsible for causing various diseases in a number of animal species (Rogers and others 1992, Cheon and Chae 1996, Chanter 1997, Vancanneyt and others 2001). In particular, Enterococcus hirae has been associated with endocarditis in broilers (McNamee and King 1… Leer más

Vela AI., Fernandez A., Moreno B., Casamayor A., Chacon G., Villa A., Comenge J. and Fernandez-Garayzabal JF..

August 26th, 2010

Polyclonal Aspergillus fumigatus infection in captive penguins

Veterinary Microbiology 144(3-4):444-449.

     Aspergillus fumigatus is a known opportunistic pathogen of penguins. Most reports of penguin aspergillosis have focused on clinical, histopathological and microbiological findings. The molecular characterization of A. fumigatus isolates involved in invasive infections in penguins has yet to be addressed. The aim of this work was to study the possible coexistence of different A. fumigatus genotypes in five clinical cases of invasive aspergillosis in captive penguins. Differences in other relevant characteristics of the isolates, including mating type and invasiveness, were also considered.

Alkaline protease and elastase production by the A. fumigatus isolates was evaluated by plate assays. Random amplified polymorphic DNA, and… Leer más

Alvarez-Perez S., Mateos A., Dominguez L., Martinez-Nevado E., Blanco JL. and Garcia ME.

August 1st, 2010

Identifying areas for infectious animal disease surveillance in the absence of population data: highly pathogenic avian influenza in wild bird populations of Europe

Preventive Veterinary Medicine 96(1-2):1-8.

     A large number (n=591) of H5N1 highly pathogenic avian influenza virus (HPAIV) outbreaks have been reported in wild birds of Europe from October 2005 through January 2009. Consequently, prevention and control strategies have been implemented in response to the outbreaks and considerable discussion has taken place regarding the need for implementing surveillance programs in high-risk areas with the objective of early detecting and preventing HPAIV epidemics. However countries ability to define the temporal and spatial extension of the high risk areas has been impaired by the lack of information on the distribution of susceptible wild bird populations in the region. Here, a technique for the detection of time-space disease clustering that doe… Leer más

Iglesias I., Perez AM., de la Torre A., Munoz MJ., Martinez-Aviles M. and Sanchez-Vizcaino JM..

August 1st, 2010

First Case of Highly Pathogenic Avian Influenza in Poultry in Spain

Transboundary And Emerging Diseases 57(4):282-285.

     Summary In October 2009, highly pathogenic avian influenza virus (HPAIV) was isolated for the first time in poultry in Spain. Sequencing analysis revealed that it was an H7N7 HPAIV. The progenitors of H7 HPAIV strains involved in recent European poultry outbreaks were simultaneously circulating in wild birds. The infected Spanish farm is located close to a reservoir abundant in wild birds. Epidemiological investigation found no links to other poultry holdings and those located in the control area were negative for AIV. Previous spatial risk analyses had identified the area where the infected holding is located to beat high relative risk for the introduction and presence of H5N1 HPAIV by wild birds. We suggest a risk-based surveillance schem… Leer más

Iglesias I., Martinez-Aviles M., Munoz MJ., de la Torre A. and Sanchez-Vizcaino JM..

August 1st, 2010

A simulation model for the potential spread of foot-and-mouth disease in the Castile and Leon region of Spain

Preventive Veterinary Medicine 96(1-2):19-29.

     A spatial stochastic model was used to simulate the spread of a foot-and-mouth disease (FMD) epidemic in the Castile-and-Leon (CyL) region of Spain. The model was fitted using information available on premises demographics and on assumptions for animal movements, indirect contacts, and airborne exposure. Control measures dictated by Spanish and European Union regulations constituted a reference strategy to which six alternative control strategies were compared. For the reference strategy, the median (95% PI) numbers of infected, depopulated, and quarantined premises were 141 (2-1099), 164 (4-1302), and 334 (31-2059), respectively. Depopulation and vaccination of premises within a radius of <1km and <3km, respectively, around infected premis… Leer más

Martinez-Lopez B., Perez AM. and Sanchez-Vizcaino JM..

August 1st, 2010

Presence of herpesvirus in striped dolphins stranded during the cetacean morbillivirus epizootic along the Mediterranean Spanish coast in 2007

Archives Of Virology 155(8):1307-11.

     A screening for herpesvirus (HV) was carried out using a tissue bank obtained from the cetacean morbillivirus (CeMV) mortality episode that occurred along the Mediterranean Spanish coast in 2007. A total of 14 cetaceans, including six long-finned pilot whales and eight striped dolphins, were studied using histopathology and molecular analysis to detect HV and CeMV. In five of the eight dolphins (62.5%) infected with CeMV, eight novel HV sequences were also detected. No HV lesions were found in any of the coinfected dolphins, which may indicate that HV did not contribute to the mortality in the CeMV outbreak. This is the first report of HV infection in any cetacean from the Mediterranean Sea… Leer más

Belliere EN., Esperon F., Arbelo M., Munoz MJ., Fernandez A. and Sanchez-Vizcaino JM..

July 29th, 2010

Experimental infection of Eurasian wild boar with Mycobacterium avium subsp. avium

Veterinary Microbiology 144(1-2):240-245.

     The Eurasian wild boar (Sus scrofa) is increasingly relevant as a host for several pathogenic mycobacteria. We aimed to characterize the first experimental Mycobacterium avium subsp. avium (MAA) infection in wild boar in order to describe the lesions and the immune response as compared to uninfected controls. Twelve 1-4-month-old wild boar piglets were housed in class III bio-containment facilities. Four concentrations of MAA suspension were used: 10, 10(2) and 10(4) mycobacteria (2 animals each, oropharyngeal route) and 2.5x10(6) mycobacteria (2 animals each by the oropharyngeal and nasal routes). No clinical signs were observed and pathology evidenced a low pathogenicity of this MAA strain for this particular host. Bacteriological and pat… Leer más

Garrido JM., Vicente J., Carrasco-Garcia R., Galindo RC., Minguijon E., Ballesteros C., Aranaz A., Romero B., Sevilla I., de la Fuente J. and Gortazar C.

July 29th, 2010

Molecular characterization of Mycobacterium avium subspecies paratuberculosis Types II and III isolates by a combination of MIRU-VNTR loci

Veterinary Microbiology 144:118-126.

     Mycobacterial interspersed repetitive units and variable number tandem repeats typing (MIRU-VNTR) is a useful technique that has been recently applied to characterize members of the Mycobacterium avium complex (MAC). The aim of this study was to examine the genetic variability among a collection of Spanish M. avium subspecies paratuberculosis (M. a. paratuberculosis) isolates with a combination of MIRU-VNTR loci. For this purpose we tested six MIRU-VNTR loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32, VNTR-292 and VNTR-259) in 70 M. a. paratuberculosis isolates of Types II and III that were recovered from 22 Spanish localities during a nine-year period (1998-2007). The combination of five loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32 and VNTR-259) enabled … Leer más

Castellanos E., Romero B., Rodriguez-Campos S., de Juan L., Bezos J., Mateos A., Dominguez L. and Aranaz A.

July 1st, 2010

Immunohistochemical Detection of Bluetongue Virus in Fixed Tissue

Journal Of Comparative Pathology 143(1):20-28.

     The VP7 structural protein is the most abundant of the major core proteins and is highly conserved in all serotypes of bluetongue virus (BTV). The aim of this study was to develop immunohistochemical techniques for the detection of BTV VP7 in Bouin`s- and formalin-fixed and paraffin wax-embedded tissues from small ruminants (sheep and goats) naturally infected with BTV. Tissue samples were taken from animals in which BTV infection had been confirmed by reverse transcriptase polymerase chain reaction. Optimal results were obtained by incubation of monoclonal antibody 2E9 on samples fixed with Bouin`s solution or neutral buffered formalin. Optimum antigen retrieval for Bouin`s-fixed samples was by microwave heating (6min) of tissue samples in… Leer más

Sanchez-Cordon PJ., Rodriguez B., Risalde MA., Molina V., Pedrera M., Sanchez-Vizcaino JM. and Gomez-Villamandos JC.

June 3rd, 2010

Multiresistant Salmonella enterica serovar 4,[5], 12:i:- in Europe: a new pandemic strain?

Eurosurveillance 15(22):19580.

     A marked increase in the prevalence of S. enterica serovar 4,[5],12:i:- with resistance to ampicillin, streptomycin, sulphonamides and tetracyclines (R-type ASSuT) has been noted in food-borne infections and in pigs/pig meat in several European countries in the last ten years. One hundred and sixteen strains of S. enterica serovar 4,[5],12:i:- from humans, pigs and pig meat isolated in England and Wales, France, Germany, Italy, Poland, Spain and the Netherlands were further subtyped by phage typing, pulsed-field gel electrophoresis and multilocus variable number tandem repeat analysis to investigate the genetic relationship among strains. PCR was performed to identify the fljB flagellar gene and the genes encoding resistance to ampicillin, … Leer más

Hopkins KL., Kirchner M., Guerra B., Granier SA., Lucarelli C., Porrero MC., Jakubczak A., Threlfall EJ. and Mevius DJ.

June 1st, 2010

Short communication. First detection of Israeli Acute ParalysisVirus (IAPV) in Spanish honeybees

Spanish Journal Of Agricultural Research 8(2):308-311.

     Spanish bee samples were analyzed for the presence of Israel Acute Paralysis Virus (IAPV). Some of these samples were collected from colonies presenting compatible symptoms with the colony collapse disorder (CCD, 240 out of 484) and the rest were asymptomatic. Only one of these samples was diagnosed as positive to IAPV by employing a one step RT-PCR that targets the ORF 2 of the IAPV genome. Specificity of the RT-PCR assay was evaluated by sequence analysis of size specific amplification products. IAPV nucleotide sequences already published in GeneBank were used to construct a phylogenetic tree that included the new Spanish IAPV sequence (FJ821506). They segregated in two main lineages and the Spanish isolate was mainly related with the Ame… Leer más

Kukielka D. and Sanchez-Vizcaino JM..

June 1st, 2010

Characterization of a recombinant transferrin-binding protein A (TbpA) fragment from Haemophilus parasuis serovar 5

FEMS Microbiology Letters 307(2):142-150.

     Haemophilus parasuis, the etiological agent of Glässer`s disease in pigs, possesses iron acquisition pathways mediated by a surface receptor that specifically bind porcine transferrin. This receptor is composed of transferrin-binding protein A (TbpA) and TbpB. As it has been reported for other gram-negative organisms, H. parasuis TbpA could be useful as a candidate target for H. parasuis vaccination. In this study, a 600-bp tbpA fragment of the gene encoding TbpA from H. parasuis serovar 5, the Nagasaki strain, was amplified by PCR and cloned into a pBAD/Thio-TOPO expression vector, generating the pBAD-Thio-TbpA-V5-His (TbpA-His) construction. Escherichia coli LMG194-competent cells were transformed with this construction, followed by the i… Leer más

Martinez S., Frandoloso R., Rodriguez-Ferri E., Gonzalez-Zorn B. and Gutierrez CB.

June 1st, 2010

Isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens): a case report

Veterinarni Medicina 55(6):297-301.

     This paper reports the isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens). The microorganism was isolated from cutaneous lesions, identified by the commercial API 20 C AUX system, and confirmed by sequencing. Topical treatment with sertaconazol resulted in complete clinical recovery of the animal and repeat testing did not result in the recovery of the yeast from the healed lesion sites.… Leer más

Alvarez-Perez S., Mateos A., Dominguez L., Martinez-Nevado E., Blanco JL. and Garcia ME.

June 1st, 2010

Determination of best tissues and culture media for microbiological confirmationof the serological results of the control campaigns of Brucella melitensis in sheep

Archivos De Medicina Veterinaria 42(2):43-9.

     Slaughtering seropositive animals in order to control ovine brucellosis requires a microbiological culture as a confirmatory technique. It is necessary to establish the most adequate tissues, due to difficulties involved in performing microbiological techniques in a large number of animals and samples. In this sense, there is a lack of data obtained from naturally infected sheep representatives of the different epidemiological situations, as recommended by OIE. In our study we carried out the necropsy of 92 ewes suspicious to be infected by Brucella and we performed microbiological culture and identification of 10 tissues per animal. Animals were classified according to an epidemiological survey as belonging to chronic brucellosis flocks, a… Leer más

Alvarez L., Duran M., Gomez B., Garrido F., Yustes C., Vendrell J., Dominguez L., Fernandez-Garayzabal JF., Vela AI. and Latre MV.

May 29th, 2010

Absence of TB in Iberian ibex (Capra pyrenaica) in a high-risk area

The Veterinary Record 166(22):700.

     Mycobacterium bovis is the causative agent of bovine tuberculosis (TB); it has an exceptionally wide host range, which includes goats. In fact, caprine tuberculosis caused by M bovis has been widely studied in domestic goats due to their high susceptibility to infection (O’Reilly and Daborn 1995, Daniel and others 2009, Quintas and others 2010). However, little is known about bovine TB in wild Caprinae. To our knowledge, there has been only a single published report about the presence of this disease in a wild caprine population. In that study, one eight-year-old female Iberian ibex (Capra pyrenaica) out of 450 (0.2 per cent) was diagnosed with bovine TB (Cubero and others 2002). The Iberian ibex is a medium-sized mountain ungulate endemic … Leer más

Mentaberre G., Serrano E., Velarde R., Marco I., Lavin S., Mateos A., de Juan L., Dominguez L., Olive X. and Romeva J.

May 6th, 2010

Tuberculosis in alpacas (Lama pacos) caused by Mycobacterium bovis

Journal Of Clinical Microbiology 48(5):1960-64.

     We report three cases of tuberculosis in alpacas from Spain caused by Mycobacterium bovis. The animals revealed two different lesional patterns. Mycobacterial culture and PCR assay yielded positive results for M. bovis. Molecular typing of the isolates identified spoligotype SB0295 and identical variable number tandem repeat (VNTR)allele sizes… Leer más

Garcia-Bocanegra I., Barranco I., Rodriguez-Gomez IM., Perez B., Gomez-Laguna J., Rodriguez-Campos S., Ruiz-Villamayor E. and Perea A.

May 1st, 2010

Reflection paper on MRSA in food-producing and companion animals: epidemiology and control options for human and animal health

Epidemiology And Infection 138(5):626-44.

     The scope of this reflection paper was to review the latest research on the risk of MRSA infection and colonization in animals. Attention focused on occurrence, risk factors for colonization and infection, and human contact hazard for livestock, horses, and companion animals. Whereas the clonal relationship between MRSA strains of CC398 is straightforward in livestock this is less obvious in horses. Small companion animals typically share MRSA strains that seem to exchange with a human reservoir. Management and therapeutic options have been suggested for livestock, horses, companion animals, as well as instructions on safety measures for persons in contact with
animals. Conclusions were drawn with emphasis on future research activiti… Leer más

Catry B., van Duijkeren E., Pomba MC., Greko C., Moreno MA., Pyorala S., Ruzauskas M., Sanders P., Threlfall EJ., Ungemach F. and Torren-Edo J.

May 1st, 2010

A Quantitative Assessment of the Risk for Highly Pathogenic Avian Influenza Introduction into Spain via Legal Trade of Live Poultry

Risk Analysis 30(5):798-807.

     Highly pathogenic avian influenza (HPAI) is considered one of the most important diseases of poultry. During the last 9 years, HPAI epidemics have been reported in Asia, the Americas, Africa, and in 18 countries of the European Union (EU). For that reason, it is possible that the risk for HPAI virus (HPAIV) introduction into Spain may have recently increased. Because of the EU free-trade policy and because legal trade of live poultry was considered an important route for HPAI spread in certain regions of the world, there are fears that Spain may become HPAIV-infected as a consequence of the legal introduction of live poultry. However, no quantitative assessment of the risk for HPAIV introduction into Spain or into any other EU member state … Leer más

Sanchez-Vizcaino F., Perez AM., Lainez M. and Sanchez-Vizcaino JM..

April 1st, 2010

16S rRNA Methyltransferase RmtC in Salmonella enterica Serovar Virchow

Emerging Infectious Diseases 16(4):712-715.

     We screened Salmonella and Escherichia coli isolates, collected 2004–2008 in the United Kingdom, for 16S rRNA methyltransferases. rmtC was identified in S. enterica serovar Virchow isolates from clinical samples and food. All isolates were clonally related and bore the rmtC gene on the bacterial chromosome. Surveillance for and research on these resistance determinants are essential.… Leer más

Hopkins KL., Escudero JA., Hidalgo L. and Gonzalez-Zorn B..

April 1st, 2010

Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types using real-time PCR and high resolution melt analysis of the MAP1506 gene

Journal Of Clinical Microbiology 48(4):1474-7.

     High resolution melt (HRM) can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (Proline-Proline-Glutamic acid) gene family… Leer más

Castellanos E., Aranaz A. and De Buck J.

April 1st, 2010

Haemophilus influenzae clinical isolates with plasmid pB1000 bearing blaROB-1. Fitness cost and interspecies dissemination

Antimicrobial Agents And Chemotherapy 54(4):1506-11.

     Plasmid pB1000 is a mobilizable replicon bearing the blaROB-1 ß-lactamase gene that we have recently described in Haemophilus parasuis and Pasteurella multocida animal isolates. Here, we report presence of pB1000 and a derivate plasmid, pB1000`, in four H. influenzae clinical isolates from human origin. Pulsed-field gel electrophoresis showed unrelated patterns in all strains, indicating that existence of pB1000 in H. influenzae isolates is not the consequence of clonal dissemination. The replicon can be transferred both by transformation and conjugation into H. influenzae, giving rise to ampicillin (MIC > 64 μg/ml) and cefaclor (MIC > 64 μg/ml) resistant recipients. Stability experiments showed that pB1000 is stable in H. influen… Leer más

San Millan A., Garcia-Cobos S., Escudero JA., Hidalgo L., Gutierrez B., Carrilero L., Campos J. and Gonzalez-Zorn B..

March 16th, 2010

Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization

BMC Microbiology 10:79.

     Lactococcus garvieae is a bacterial pathogen that affects different animal species in addition to humans. Despite the widespread distribution and emerging clinical significance of L. garvieae in both veterinary and human medicine, there is almost a complete lack of knowledge about the genetic content of this microorganism. In the present study, the genomic content of L. garvieae CECT 4531 was analysed using bioinformatics tools and microarray-based comparative genomic hybridization (CGH) experiments. Lactococcus lactis subsp. lactis IL1403 and Streptococcus pneumoniae TIGR4 were used as reference microorganisms.

The combination and integration of in silico analyses and in vitro (CGH) experiments performed in comparison with t… Leer más

Aguado-Urda M., Lopez-Campos GH., Fernandez-Garayzabal JF., Martin-Sanchez F., Gibello A., Dominguez L. and Blanco MM.

March 1st, 2010

Bluetongue virus serotypes 1 and 4 in red deer, Spain

Emerging Infectious Diseases 16(3):518-20.

     We studied the potential of red deer as bluetongue maintenance hosts and sentinels. Deer maintained detectable bluetongue virus (BTV) serotype 4 RNA for 1 year after the virus was cleared from livestock. However, the virus was not transmitted to yearlings. BTV serotype 1 RNA was detected in red deer immediately after its first detection in cattle… Leer más

Rodriguez B., Gortazar C., Ruiz-Fons F. and Sanchez-Vizcaino JM..

February 24th, 2010

Detection of bluetongue serotype 4 in mouflons (Ovis aries musimon) from Spain

Veterinary Microbiology 141:164-7.

     Bluetongue serotype 4 (BTV4) has been detected for the first time in tissue samples from 2 mouflons (Ovis aries musimon) from the South of Spain, in a retrospective study. The samples included in this study had been fixed and paraffin-embedded for over a year prior to their analysis using a BTV group-specific and a BTV4-specific RT-PCR test. Lung and lymphatic nodes were found positive in both specimens. The amplified DNA was confirmed to be BTV4 by sequencing the RT-PCR products and comparing them with other sequences from GenBank. The combination of RNA extraction from paraffin-embedded samples and serotype-specific real-time RT-PCR assays provides the tools for the detection of BTV from samples stored for a long time. The results shown i… Leer más

Rodriguez B., Sanchez-Cordon PJ., Molina V., Risalde MA., Perez de Diego AC., Gomez-Villamandos JC. and Sanchez-Vizcaino JM..

February 24th, 2010

High spoligotype diversity within a Mycobacterium bovis population: Clues to understanding the demography of the pathogen in Europe

Veterinary Microbiology 141(1-2):89-95.

     Mycobacterium bovis is the main causative agent of bovine tuberculosis. This zoonotic disease produces important economic losses and must be considered a threat to endangered animal species and public health. This study was performed (1) to assess the degree of diversity of the Spanish M. bovis isolates and its effect on the epidemiology of the infection, and (2) to understand the connection of M. bovis populations within a European context. In this report we resume the DVR-spoligotyping results of 6215 M. bovis isolates collected between 1992 and 2007 from different hosts. The isolates clustered into 252 spoligotypes which varied largely in frequency, geographical distribution and appearance in different animal species. In general, the mos… Leer más

Rodriguez-Campos S., Romero B., Bezos J., de Juan L., Alvarez J., Castellanos E., Moya N., Lozano F., Gonzalez S., Saez-Llorente JL., Mateos A., Dominguez L. and Aranaz A.

February 15th, 2010

Experimental infection with Mycobacterium caprae in goats and evaluation of immunological status in tuberculosis and paratuberculosis co-infected animals

Veterinary Immunology And Immunopathology 133(2-4):269-275.

     Tuberculosis in goats (caused by Mycobacterium caprae and M. bovis) has become a significant concern in recent years because of its high prevalence in certain caprine herds in Spain and other European countries, and also due to the potential transmission to other animals and human beings. In the present study, a transthoracic model of tuberculosis infection was performed on goats. Animals were selected based on the serological response used to detect paratuberculosis in goats (negative and positive results). The kinetics of the immune response was evaluated using the interferon-gamma (IFN-gamma) assay, skin tests and serology of paratuberculosis during nine months post-challenge. At the end of the study the animals were necropsied, tubercul… Leer más

Bezos J., de Juan L., Romero B., Alvarez J., Mazzucchelli F., Mateos A., Dominguez L. and Aranaz A.

February 1st, 2010

Avian mycobacteriosis in free-living raptors in Majorca Island, Spain

Avian Pathology: Journal Of The W.V.P.A 39(1):1-6.

     Avian mycobacteriosis is a chronic, infectious disease caused by different species of mycobacteria, usually belonging to the Mycobacterium avium complex. From 2004 to 2007, 589 raptors brought dead or sick to a wildlife rehabilitation centre in Majorca (Balearic Islands, Spain) were necropsied. The birds belonged to 12 different species, chiefly common kestrel (Falco tinnunculus) (n=297), scops owl (Otus scops) (n=109), barn owl (Tyto alba) (n=75), long-eared owl (Asio otus) (n=58), peregrine falcon (Falco peregrinus) (n=27), and booted eagle (Hieraaetus pennatus) (n=13). Gross lesions compatible with mycobacteriosis were observed in 14 birds (2.4%) found in several locations in Majorca. They were 12 kestrels (prevalence in this species, 4.… Leer más

Millan J., Negre N., Castellanos E., de Juan L., Mateos A., Parpal L. and Aranaz A.

February 1st, 2010

Resistance mechanisms and farm-level distribution of fecal Escherichia coli isolates resistant to extended-spectrum cephalosporins in pigs in Spain

Research In Veterinary Science 88:83-87.

     INTRODUCTION: Fecal Escherichia coli isolates showing a phenotype of reduced susceptibility or resistance to extended-spectrum cephalosporins are common among pigs in Spain. The aim of this study was to describe the main beta-lactam resistance mechanisms carried by these strains and their distribution at farm-level. MATERIALS AND METHODS: Twenty-nine E. coli isolates showing reduced susceptibility or resistance to extended-spectrum cephalosporins were collected from a sampling frame of 80 pig farms distributed over 13 Spanish provinces. The survey was carried out at the slaughterhouse level in 2004. RESULTS: Of the 29 isolates, 21 (72%) met the criteria for a positive phenotypic confirmatory test for extended-spectrum beta-lactamases (ESBL)… Leer más

Escudero E., Vinue L., Teshager T., Torres C. and Moreno MA.

January 1st, 2010

Percentage of reactor animals to single comparative cervical intradermal tuberculin (SCCIT) in small ruminants in Punjab Pakistan

Acta Tropica 113(1):88-91.

     To investigate the percentage reactor animals to SCCIT in sheep and goat the present study was carried out by using bovine and avian PPDs at 7 Livestock Experiment Stations of Punjab and villages around two cities. The overall percentage of reactor animals to SCCIT at farms in sheep and goat was 0.9% and 2.4%, while it was 0.4% and 0.0% around two cities, respectively. Hundred percent of goat and 86% of sheep farms under study had positive reactor animals. The reactor animals to SCCIT around two cities were 0% in goat and 0.4% in sheep. Goats (P<0.05) and sheep (P<0.001) kept with large ruminants at farms had higher percentage of reactor animals. Age showed significant (P<0.01) association with tuberculosis in sheep and goat. In goats of mo… Leer más

Javed MT., Munir A., Shahid M., Severi G., Irfan M., Aranaz A. and Cagiola M.

January 1st, 2010

Streptococcus porci sp. nov., isolated from swine sources

International Journal Of Systematic And Evolutionary Microbiology 60(Pt 1):104-8.

     Two unidentified Gram-positive, catalase-negative, coccus-shaped organisms were recovered from pigs and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Streptococcus, although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequence studies confirmed this identification and showed that the nearest phylogenetic relatives of the unknown cocci were Streptococcus plurextorum 1956-02(T) and Streptococcus suis NCTC 10234(T) (97.9 and 96.0 % 16S rRNA gene sequence similarity, respectively). The new isolates were related most closely to S. suis CIP 103217(T) based on rpoB gene sequence analysis (<8… Leer más

Vela AI., Perez-Sancho M., Zamora L., Palacios L., Dominguez L. and Fernandez-Garayzabal JF..

Science Publication
ISI Scientific Publications
Total Last 60 mo. Last 12 mo.
1 ZTA 250 SUAT 63 SUAT 16
2 ICM 239 ZTA 53 SAP 11
3 MYC 227 MYC 49 MYC 10
4 SUAT 220 ICM 42 ZTA 6
5 NED 75 SAP 31 ICM 6
  VISAVET 1127 319 57

Impact Factor Average
Total Last 60 mo. Last 12 mo.
1 ZTA 3.907 ZTA 5.359 ZTA 6.183
2 SAP 3.818 SUAT 4.202 SAP 6.06
3 SUAT 2.988 SAP 4.095 NED 4.3
4 MYC 2.8 NED 3.858 SUAT 3.713
5 DICM 2.776 ICM 3.776 MYC 3.19
VISAVET 3.284 4.873 6.545

ISI Scientific Publications
Total Last 60 mo.
1 Transbound Emerg Dis 93 Front Vet Sci 36
2 Vet Microbiol 88 Transbound Emerg Dis 32
3 Prev Vet Med 77 Animals 15
4 Front Vet Sci 62 Res Vet Sci 14
5 PLoS ONE 56 Sci Rep 14
  All journals 355   All journals 39